Selective reduction of the interaction of magnetic nanoparticles with leukocytes and tumor cells by human plasma

Schwalbe, Manuela; Jörke, Cornelia; Buske, N.; Höffken, K.; Pachmann, Katharina; Clement, Joachim H.

doi:10.1016/j.jmmm.2005.02.037

Cited by 18 publications

(17 citation statements)

References 9 publications

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“…27 It has been reported that MNPs are normally ingested via endocytosis into phagosomes, which eventually fuse with lysosomes for degradation because of the acid environment. 28 The products of MNP degradation (Fe ions) are responsible for the production of ROS. However, in this respect, the experimental data indicated that the range of concentration 1-10 µg/mL induced negligible cell toxicity as the amount of intracellular Fe ions was not far from the physiological level and was lower than those resulting from the use of commercial MNPs such as Resovist and Endorem.…”

Section: Resultsmentioning

confidence: 99%

Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

Riggio

Calatayud

Hoskins

et al. 2012

IJN

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Purpose: It has been proposed in the literature that Fe 3 O 4 magnetic nanoparticles (MNPs) could be exploited to enhance or accelerate nerve regeneration and to provide guidance for regenerating axons. MNPs could create mechanical tension that stimulates the growth and elongation of axons. Particles suitable for this purpose should possess (1) high saturation magnetization, (2) a negligible cytotoxic profile, and (3) a high capacity to magnetize mammalian cells. Unfortunately, the materials currently available on the market do not satisfy these criteria; therefore, this work attempts to overcome these deficiencies. Methods: Magnetite particles were synthesized by an oxidative hydrolysis method and characterized based on their external morphology and size distribution (high-resolution transmission electron microscopy [HR-TEM]) as well as their colloidal (Z potential) and magnetic properties (Superconducting QUantum Interference Devices [SQUID]). Cell viability was assessed via Trypan blue dye exclusion assay, cell doubling time, and MTT cell proliferation assay and reactive oxygen species production. Particle uptake was monitored via Prussian blue staining, intracellular iron content quantification via a ferrozine-based assay, and direct visualization by dual-beam (focused ion beam/scanning electron microscopy [FIB/SEM]) analysis. Experiments were performed on human neuroblastoma SH-SY5Y cell line and primary Schwann cell cultures of the peripheral nervous system. Results: This paper reports on the synthesis and characterization of polymer-coated magnetic Fe 3 O 4 nanoparticles with an average diameter of 73 ± 6 nm that are designed as magnetic actuators for neural guidance. The cells were able to incorporate quantities of iron up to 2 pg/cell. The intracellular distribution of MNPs obtained by optical and electronic microscopy showed large structures of MNPs crossing the cell membrane into the cytoplasm, thus rendering them suitable for magnetic manipulation by external magnetic fields. Specifically, migration experiments under external magnetic fields confirmed that these MNPs can effectively actuate the cells, thus inducing measurable migration towards predefined directions more effectively than commercial nanoparticles (fluidMAG-ARA supplied by Chemicell). There were no observable toxic effects from MNPs on cell viability for working concentrations of 10 µg/mL (EC 25 of 20.8 µg/mL, compared to 12 µg/ mL in fluidMAG-ARA). Cell proliferation assays performed with primary cell cultures of the peripheral nervous system confirmed moderate cytotoxicity (EC 25 of 10.35 µg/mL). Conclusion: These results indicate that loading neural cells with the proposed MNPs is likely to be an effective strategy for promoting non-invasive neural regeneration through cell magnetic actuation.

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Section: Resultsmentioning

confidence: 99%

Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

Riggio

Calatayud

Hoskins

et al. 2012

IJN

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“…The sharpest decline was observed for the leukocytes resulting in a dramatic gap in the separation rates of tumor cells versus leukocytes. The amount of labeled leukocytes could be further reduced by addition of 0.5-5% human plasma without affecting the amount of tumor cells seriously (Schwalbe et al 2005). Thus modification of the incubation conditions helps to improve depletion of tumor cells from leukocytes from peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Clement

Schwalbe

Buske³

et al. 2006

J Cancer Res Clin Oncol

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“…But this effect is independent of the cell type and the incubation time (Fig. 2B) within an incubation period of 20 min, allows the high enrichment of tumor cells from leukocytes [9,11]. In contrast to commercial immunomagnetic separation techniques the short-term incubation procedure works without antibodies directed against tumorassociated antigens.…”

Section: Resultsmentioning

confidence: 98%

“…Tumor cells show a rapid and intense interaction with CM-dextran-coated nanoparticles whereas leukocytes exhibited a delayed tendency to interact [11].…”

Section: Resultsmentioning

confidence: 99%

“…We could previously show that short-term application of carboxymethyl dextran-coated magnetic nanoparticles allows the separation of human cells in a time, concentration and cell-type specific manner [9,10]. The cell-type specific interaction of these nanoparticles could be further modified by variation of the incubation conditions [11]. Dextran seems to be the most suitable polysaccharide for coating magnetic nanoparticles for interacting with human cells [12].…”

Section: Introductionmentioning

confidence: 99%

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Magnetic nanoparticles coated with carboxymethylated polysaccharide shells—Interaction with human cells

Wotschadlo

Liebert

Heinze

et al. 2009

Journal of Magnetism and Magnetic Materials

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Selective reduction of the interaction of magnetic nanoparticles with leukocytes and tumor cells by human plasma

Cited by 18 publications

References 9 publications

Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

Poly-l-lysine-coated magnetic nanoparticles as intracellular actuators for neural guidance

Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells

Magnetic nanoparticles coated with carboxymethylated polysaccharide shells—Interaction with human cells

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