Selective Recovery of Proteins by Control of Their Surface Properties Utilizing PEG-Bound Affinity Ligands.

Kuboi, Ryoichi; Hasegawa, Tetsuhiro; Yamahara, Katsuhito; Komasawa, Isao; Johansson, Gillis

doi:10.1252/jcej.31.618

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…Palmitic acid in PEG-palmitate showed not only high affinity to Bacillus subtilis neutral protease but also an activation effect (factor of 2.5) on the proteolytic activity. Kuboi et al (1998) investigated the different ligands for selective recovery of proteins in PEG/dextran aqueous two-phase system. The ligands used were nonionic surfactant (Triton and PEG-palmitate) and PEG-bound dye (Cibacron Blue and Procion Yellow).…”

Section: The Other Ligands Affinity Partitioningmentioning

confidence: 99%

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Souza

Pontes

et al. 2003

Braz. arch. biol. technol.

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From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phasesystem with other techniques for more effective purification of enzymes are also presented.

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Section: The Other Ligands Affinity Partitioningmentioning

confidence: 99%