Selective Recovery of Anthocyanins and Hydroxycinnamates from a Byproduct of Citrus Processing

Abstract

The adsorption-desorption performances of commercial resins (two polystyrene-divinylbenzene copolymers and one methacrylic) were tested in column studies for the selective recovery of anthocyanins and hydroxycinnamates from pigmented pulp wash (PW), a byproduct of blood orange juice processing. Methanol, ethanol, and their mixtures with different percentages of water as eluents were tested in order to investigate the selective desorption of these natural antioxidants with the goal of minimizing the presence of other compounds, mostly flavanones and limonoids, in the concentrated eluates. The results indicated that polystyrene-divinylbenzene resins were able to adsorb a major amount of anthocyanins and hydroxycinnamates and to desorb them in more-concentrated fractions. No selectivity was observed using pure methanol and ethanol which resulted in concentrates with no further separation of components from the starting raw PW. The ethanol/water concentrates showed higher yields than the fractions attained using methanol/water; however, a lower selectivity toward anthocyanin pigments was observed. The best performing resin, EXA-118, together with the mixture methanol/water 50:50 (v/v) as best eluent, appears to be the most suitable system to obtain highly concentrated extracts. Thus, it was chosen to perform a larger experiment, to analyze the selectivity of the removal upon an increase in elution volume.

Sustainable sunflower processing — I. Development of a process for the adsorptive decolorization of sunflower [Helianthus annuus L.] protein extracts

“…The application of adsorbent resins consisting of acrylic, methacrylic or styrene-divinylbenzene copolymer matrices has been shown to be a valuable tool for polyphenol removal from liquid extracts (Bretag et al, 2009a,b;Kammerer et al 2007;Scordino et al, 2005;Schieber et al, 2003). The profitability of an industrial process for the adsorptive recovery of phenolic compounds from sunflower protein extracts mainly depends on the adsorption capacity and the period of time required for this process.…”

Recovery of antioxidants from industrial waste liquors using membranes and polymeric resins

“…Alternatively, polymeric resins have been used for the separation and purification of phenolic compounds (anthocyanins, flavonoids and hydroxycinnamates) from natural products (Llorach et al, 2004;Saleh et al, 2008;Scordino et al, 2005). Anthocyanins from grape pomace were obtained at lab and pilot plant scales by adsorption in a styrene-divinylbenzene resin, and eluted with alcoholic solutions (Kammerer et al, 2005); whereas polyphenols from apple juice were eluted with water, ethanol or methanol (Saleh et al, 2008) Coupled membrane processing and adsorption has been proposed to recover or to remove phenolic compounds from tea solutions (Li et al, 2005) or alfalfa protein concentrates (D'Alvise et al, 2000).…”

Resin Adsorption and Ion Exchange to Recover and Fractionate Polyphenols

“…Such differences in the binding behavior of individual compounds were even observed upon upscaling of the process. Accordingly, this technology is also applicable for industrial production of phenolic preparations and fractionation of complex phenolic mixtures (Kammerer et al, 2010c;Scordino et al, 2005).…”