Selective production of resistant sporangia in suspensions of Allomyces

Youatt, Jean

doi:10.1016/s0007-1536(80)80101-x

Cited by 8 publications

(18 citation statements)

References 8 publications

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“…Therefore it is glutamic acid which prevents the development of zoosporangia in the glucoseglutamic acid solution. It appears that the transcription of mRNA was inhibited, since a transcription at 4 h was necessary for the development of resistant sporangia (Youatt 1980a).…”

Section: Discussionmentioning

confidence: 99%

“…Detailed descriptions of the culture methods and microscopic observations have been reported (Youatt 1980a). The fractions analysed in this paper were identical with those described previously (Youatt 1980b).…”

Section: Methodsmentioning

confidence: 98%

“…Allomyces macrogynus was induced to make zoosporangia, by suspension of vegetative plants in distilled water or resistant sporangia in glucose-glutamic acid solution (Youatt 1980a). Analysis of the supernatant media and the contents of the plants showed that plants which produced zoosporangia degraded RNA during this development, releasing phosphate, uracil, guanine and hypoxanthine.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Carbohydrates of Allomyces Macrogynus During the Selective Development of Either Zoosporangia or Resistant Sporangia

Youatt¹

1980

Aust. Jnl. Of Bio. Sci.

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Allomyces macrogynus synthesized trehalose but did not appear to metabolize it. Trehalose was released to the suspending medium before the development of zoosporangia by plants suspended in water and before the development of resistant sporangia by plants in glutamic acid and glucose solution. Plants in the latter solution accumulated high levels of glycogen and trehalose before mRNA was transcribed for the development of resistant sporangia. Before the transcription of mRNA for either zoosporangia or resistant sporangia there was a period during which the plant content of glycogen, trehalose, protein and nucleic acid was constant. The increase in wall glycans and chitin during the development of zoosporangia was measured and possible origins of the walls are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Changes in the Carbohydrates of Allomyces Macrogynus During the Selective Development of Either Zoosporangia or Resistant Sporangia

Youatt¹

1980

Aust. Jnl. Of Bio. Sci.

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“…The use of cycloheximide (Horowitz et al 1970) or 8-hydroxyquinoline (Youatt 1980) is then recommended, especially in slightly acidic growth media (pH 5-6) (Fraser and Creanor 1975). 8-Hydroxyquinoline acts as a chelator of Mg 2+ ions, which affects m-RNA synthesis more than proteosynthesis (Fraser and Creanor 1974).…”

Section: Discussionmentioning

confidence: 99%

The inhibition of clavine biosynthesis by 5-fluorotryptophan; a useful tool for the study of regulatory and biosynthetic relationships in Claviceps

Pažoutová

Křen

Sajdl

1990

Appl Microbiol Biotechnol

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Clavine alkaloid production in submerged cultures of various Claviceps strains was inhibited by 0.25 m M 5-fluorotryptophan (5-Ftrp). The inhibition was removed by washing the cultures, or by addition of 50 m M tryptophan. Lower doses of tryptophan (0.25-25.0 raM) were ineffective. Addition of cycloheximide a n d / o r 8-hydroxyquinoline (8-HOQ) to the inhibited cultures 24 and 48 h prior washing increased the rate of alkaloid synthesis in washed cultures (superinduction) and the rate of clavine synthesis was proportional to the length of 8-HOQ pretreatmento The addition of 0.25 m M tryptophan in washed cultures also stimulated alkaloid synthesis, but only for 2-3 days, then production in the cultures without tryptophan was higher. Cultures of Claviceps strains 59 and EK-10 inhibited with 5-Ftrp were capable of converting chanoclavine into further clavine pathway products. 5-Fluorotryptophan probably acts as an inhibitor of the first enzyme of the clavine pathway. However, the superinduction suggests the presence of a labile regulatory protein controlling clavine biosynthesis.

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“…Since differentiation in AUomyces can take place in the absence of any exogenous nutrients, Turian (1955) suggested that intracellular proteolysis must provide the necessary nitrogen substrate during the process. Glutamate is a good source of nitrogen for AUomyces (Machlis 1953) and has some role in the differential induction of resistant sporangia as opposed to zoosporangia (Youatt 1980). Further, NH^ is well-known as an important intracelluiar regulator of various pathways besides serving as a source of nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Intracellular adenylate pools and protein degradation during zoosporangial differentiation in Allomyces arbuscula

Ojha

Turian

1985

Physiologia Plantarum

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Ojha, M, and Turian, G, 1985, Intraceiiuiar adenylate pools and protein degradation during zoosporangial differentiation in AUomyces arbuscula. Piant, In Allomyees arbuscula Butl,, strain Bali, tiie ratio of protein to dry weight remained constant in exponentially growing but decreased in differentiating cultures, Tiie adenylate pools (ATP, ADP, AMP) and energy charge whicii integrates them, increased during zoospore germination and stabilized around 0,9 during differentiation, Tiie ievei of ATP increased early during tiie induction of zoosporangia for up to 1 ii and then declined. The ADP and AMP remained low for most of the time except for a transient increase in ADP (first 30 min induction). The energy charge was low in spores. The rate of turnover of proteins during growth and differentiation was more or Sess similar for up to i,5 h after transfer. Subsequently very little turnover of proteins occurred in the growing piants. In differentiating plants, the rate of degradation was maintained and by the end of the 4 ii experimental period 30% of the vegetative proteins were degraded. The intracellular ammonium siiowed a peak between 30 to 60 min of induction and was higher in the differentiating mycelia tiian in actively growing plants, while the giutamate pooi remained around 1 [xmol (mg protein)"' in both types of plants. The physiological role of these protein degradation products is discussed.Additional key words -Adenyiate nucieotide pools, intracellular ammonium kvels, protein dry weight ratios, M. Ojha (reprint requests) and G. Turian, Lab.

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Selective production of resistant sporangia in suspensions of Allomyces

Cited by 8 publications

References 8 publications

Changes in the Carbohydrates of Allomyces Macrogynus During the Selective Development of Either Zoosporangia or Resistant Sporangia

Changes in the Carbohydrates of Allomyces Macrogynus During the Selective Development of Either Zoosporangia or Resistant Sporangia

The inhibition of clavine biosynthesis by 5-fluorotryptophan; a useful tool for the study of regulatory and biosynthetic relationships in Claviceps

Intracellular adenylate pools and protein degradation during zoosporangial differentiation in Allomyces arbuscula

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