Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires

Ali, Syed M. Usman; Alvi, Naveed ul Hassan; Ibupoto, Zafar Hussain; Nur, Omer; Willander, Magnus; Danielsson, Bengt

doi:10.1016/j.snb.2010.12.015

Cited by 116 publications

(21 citation statements)

References 37 publications

(45 reference statements)

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“…In addition, the size of the nanohexagones was estimated to be in the range between 450 and 550 nm with uniform density and spatial distribution. In principle, the diameter and length of grown ZnONWs can be tuned by a fine control over the growth process parameters such as the concentration of seed solution, the reagent stoichiometry, the temperature, and the pH of the growth solution [21] [22]. Moreover, the FESEM image following the immobilization of the ssDNA probe is shown in Figure 1(B).…”

Section: Morphologies Of Znonws Modified Gold Electrode and Immobilizmentioning

confidence: 99%

Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires

Mansor¹,

Zain²,

Hamzah³

et al. 2014

OJAB

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Herein we report an electrochemical DNA biosensor for the rapid detection of sequence (5' AAT GGA TTT ATC TGC TCT TCG 3') specific for the breast cancer 1 (BRCA1) gene. The proposed electrochemical genosensor is based on short oligonucleotide DNA probe immobilized onto zinc oxide nanowires (ZnONWs) chemically synthesized onto gold electrode via hydrothermal technique. The morphology studies of the ZnONWs, performed by field emission scanning electron microscopy (FESEM), showed that the ZnO nanowires are uniform, highly dense and oriented perpendicularly to the substrate. Recognition event between the DNA probe and the target was investigated by differential pulse voltammetry (DPV) in 0.1 M acetate buffer solution (ABS), pH 7.00; as a result of the hybridization, an oxidation signal was observed at +0.8 V. The influences of pH, target concentration, and non-complimentary DNA on biosensor performance were examined. The proposed DNA biosensor has the ability to detect the target sequence in the range of concentration between 10.0 and 100.0 µM with a detection limit of 3.32 µM. The experimental results demonstrated that the prepared ZnONWs/Au electrodes are suitable platform for the immobilization of DNA.

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Section: Morphologies Of Znonws Modified Gold Electrode and Immobilizmentioning

confidence: 99%

Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires

Mansor¹,

Zain²,

Hamzah³

et al. 2014

OJAB

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“…The addition of metal as a catalyst also improves target response and selectivity at room temperature [19]. ZnO nanorods and nanowires are particularly promising for these applications because of its large surface area, wide bandgap and exciton energy, fascinating sensitivity, biocompatibility, low weight, and resistance to rust formation [20]. For hydrogen sensing applications, surface modifications of ZnO with metal additives such as Pt, Pd, and/or Au through various techniques have been under intensive investigations [19,21,22].…”

Section: Introductionmentioning

confidence: 99%

Impact of hydrogen concentrations on the impedance spectroscopic behavior of Pd-sensitized ZnO nanorods

Kashif

Ali

et al. 2013

Nanoscale Res Lett

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ZnO nanorods were synthesized using a low-cost sol-gel spin coating technique. The synthesized nanorods were consisted of hexagonal phase having c-axis orientation. SEM images reflected perpendicular ZnO nanorods forming bridging network in some areas. The impact of different hydrogen concentrations on the Pd-sensitized ZnO nanorods was investigated using an impedance spectroscopy (IS). The grain boundary resistance (Rgb) significantly contributed to the sensing properties of hydrogen gas. The boundary resistance was decreased from 11.95 to 3.765 kΩ when the hydrogen concentration was increased from 40 to 360 ppm. IS gain curve showed a gain of 6.5 for 360 ppm of hydrogen at room temperature. Nyquist plot showed reduction in real part of impedance at low frequencies on exposure to different concentrations of hydrogen. Circuit equivalency was investigated by placing capacitors and resistors to identify the conduction mechanism according to complex impedance Nyquist plot. Variations in nanorod resistance and capacitance in response to the introduction of various concentrations of hydrogen gas were obtained from the alternating current impedance spectra.

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“…The sensor electrodes were constructed following the method as previously [76]. The plastic substrate was affixed on a support in the vacuum chamber of an evaporator.…”

Section: Potentiometric Biosensors Based On Lipid Stabilized Membranementioning

confidence: 99%

“…A uric acid electrochemical device was reported in the literature by immobilizing the enzyme uricase into polymerized lipid membranes on Zn nanowires [76]. The enzyme was co-deposited with the lipid membrane prior to polymerization on the surface of the electrode.…”

Section: Applications Of the Zno Nanoelectrode-based Devicesmentioning

confidence: 99%

Recent Lipid Membrane-Based Biosensing Platforms

et al. 2019

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The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

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Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires

Cited by 116 publications

References 37 publications

Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires

Detection of Breast Cancer 1 (BRCA1) Gene Using an Electrochemical DNA Biosensor Based on Immobilized ZnO Nanowires

Impact of hydrogen concentrations on the impedance spectroscopic behavior of Pd-sensitized ZnO nanorods

Recent Lipid Membrane-Based Biosensing Platforms

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