Selective nano-sensing approach for the determination of inorganic phosphate in human urine samples

Fadhel, Alaa Abbas; Johnson, M. G.; Trieu, Khang; Koculi, Eda; Campiglia, Andrés D.

doi:10.1016/j.talanta.2016.11.017

Cited by 18 publications

(14 citation statements)

References 24 publications

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“…Pi detection is of great significance for the auxiliary diagnoses of diseases in clinical practice, so the practical application of the ICP was evaluated by the detection of Pi in urine. The concentration of Pi in urine is determined to be 4.3 mM with the ICP as shown in Table , which is within the normal level range of urinary Pi . The spiked recoveries of Pi from the urine were investigated to verify the accuracy of the detection result.…”

Section: Resultsmentioning

confidence: 97%

“…In addition, there exhibits a good linear relation- ship between the F 0 /F value of the ICP and the concentration of Pi in the range from 30 to 600 μM. Furthermore, based on 3σ/k, where σ is the standard deviation of the blank and k is the slope of the linear fitting, the detection limit of the ICP for Pi is calculated to be 5 μM which is much lower than the physiological level, [41][42][43] thus demonstrating the application potential of the ICP in biological samples.…”

Section: Fluorescent Detection Of Pimentioning

confidence: 89%

“…The concentration of Pi in urine is determined to be 4.3 mM with the ICP as shown in Table 1, which is within the normal level range of urinary Pi. [42] The spiked recoveries of Pi from the urine were investigated to verify the accuracy of the detection result. As listed in Table 1, the spiked recoveries of Pi from the urine are determined in the range from 82.0 to 115.5 %, confirming that the detection result is accurate.…”

Section: Detection Of Pi In Urinementioning

confidence: 99%

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AIE Infinite Coordination Polymer for Phosphate Ion Detection via Aggregation State Modulation

Yang

Dong

et al. 2020

ChemistrySelect

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Coordination polymers (CPs) with aggregation-induced emission (AIE) have been widely applied to fluorescent sensing based on the strategy of electron or energy transfer. Novel strategies still need to be explored to promote the application of AIE CPs in fluorescent sensing. Herein, an infinite coordination polymer (ICP), synthesized from Zn 2 + , adenine and AIE ligand 4,4'-diphenic acid (DPA), was used to detect phosphate ion (Pi) via aggregation state modulation. The ICP was decomposed in the presence of Pi due to the coordination of Pi with Zn 2 + , which disaggregated AIE ligand DPA along with fluorescence quenching. The ICP quantified Pi in the concentration range from 30 to 600 μM with a detection limit of 5 μM, which was much lower than the physiological level, and was applied to detecting Pi in urine with spiked recoveries in the range of 82.0-115.5 %. This work provides a novel strategy to design fluorescent probes with AIE CPs for the analytes exhibiting the decomposing ability, promoting the application of AIE CPs in fluorescent sensing.

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Section: Resultsmentioning

confidence: 97%

Section: Fluorescent Detection Of Pimentioning

confidence: 89%

Section: Detection Of Pi In Urinementioning

confidence: 99%

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AIE Infinite Coordination Polymer for Phosphate Ion Detection via Aggregation State Modulation

Yang

Dong

et al. 2020

ChemistrySelect

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“…The preconcentration factor, calculated as the ratio between the sample volume and the settled down NADES volume, was obtained as 71. A comparison of the analytical performances achieved by the proposed method and other methods for determination of orthophosphate is presented in Supporting Information . Compared with previous reports, the proposed method has many advantages, such as simplicity, short analysis time as well as the use of NADES as an eco‐friendly solvent with high solubilization power for both polar and nonpolar compounds and low cost.…”

Section: Resultsmentioning

confidence: 99%

Vortex‐assisted natural deep eutectic solvent microextraction using response surface methodology optimization for determination of orthophosphate in water samples by molybdenum blue method

Najafi

Hashemi

2019

J of Separation Science

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A new, rapid, and efficient microextraction technique named vortex‐assisted natural deep eutectic solvent microextraction has been developed for the preconcentration and determination of orthophosphate in real water samples. The method is based on the formation of the phosphomolybdenium blue complex followed by proposed microextraction procedure and subsequent spectrophotometric determination in a microcell. Screening study for the optimal composition of natural deep eutectic solvent was initially performed with different solvents, including choline chloride as hydrogen bond acceptor and different hydrogen bond donors. A ternary mixture of glucose‐choline chloride‐water was used as the most efficient extraction solvent. Response surface methodology based on the central composite design was used to optimize experimental parameters. Under optimal conditions, the calibration graph for orthophosphate determination was linear over the range of 2.0–80.0 µg/L (correlation coefficient of 0.9971) with a detection limit of 0.2 µg/L. The repeatability, reproducibility, and relative error values of the method were below 7%, indicating acceptable precision and accuracy. This approach, using natural deep eutectic solvent as an eco‐friendly solvent with high solubilization power and vortex mixing as an alternative energy source, represents a promising choice for a green separation and preconcentration methodology for determination of orthophosphate in real water samples.

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“…The concentration found was 570 ± 50 mg L −1 , in agreement with those reported from the analysis of the urine of healthy individuals. 34,35 ■ CONCLUSIONS Zein is a biodegradable polymer obtained as a byproduct of bioethanol production; thus, it is doubly sustainable. Here, we demonstrated that zein is a good support for reagent immobilization and stabilization in sensors for several reasons: (i) different kind of compounds can be embedded and did not interfere in the formation of the film (as happens with metallic compounds and PDMS), (ii) the enzyme remains active and…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors

2018

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Nowadays, interest in using environmentally friendly materials is increasing in many fields. However, the rational design of sensors with biodegradable materials is a challenge. The main aim of this work is to show the possibility of using zein, a protein from corn, as a biodegradable and low-cost material for immobilizing, stabilizing, and delivering different kind of reagents for developing optical sensors. Enzymes, metallic salts, and aromatic and small organic compounds were tested. In addition, different techniques of immobilization, entrapment and adsorption, were used, and different formats, such as solid devices and also multiwell platforms, were proposed. The capacity of zein for immobilizing two reagents together, enzyme and substrate, into a multianalysis format was also shown. Two applications were developed as examples: a colorimetric assay based on a ferric hydroxamate reaction for ester drugs, which was applied in atropine determination in pills, and a fluorimetric enzymatic multiwell-plate biodevice applied in phosphate determination in human serum and urine. Zein demonstrated being not only a green alternative but also a versatile polymer for developing sensors from reagents with different natures in different formats and matrices, thereby resulting in different applications.

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Selective nano-sensing approach for the determination of inorganic phosphate in human urine samples

Cited by 18 publications

References 24 publications

AIE Infinite Coordination Polymer for Phosphate Ion Detection via Aggregation State Modulation

AIE Infinite Coordination Polymer for Phosphate Ion Detection via Aggregation State Modulation

Vortex‐assisted natural deep eutectic solvent microextraction using response surface methodology optimization for determination of orthophosphate in water samples by molybdenum blue method

Delivering Inorganic and Organic Reagents and Enzymes from Zein and Developing Optical Sensors

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