Selective modulation of neuronal firing by pulse stimulations with different frequencies in rat hippocampus

Qiu, Chen; Feng, Zhouyan; Zheng, Lvpiao; Ma, Weijian

doi:10.1186/s12938-019-0700-z

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…In one study [18], neuronal stimulation had both inhibitory and excitatory effects. In addition, the stimulation frequency may affect neuronal discharge [19]. We used 3, 6, and 9 V to stimulate the hepatic branch of the vagus nerve.…”

Section: Discussionmentioning

confidence: 99%

Identification of hepatosensitive region and their neural connections in the hippocampus of rats

et al. 2022

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Background: Visceral function localization of the brain is very complex. For many years, people have been actively exploring the neural mechanism regulating visceral and substance metabolism, clarifying the complex relationship between the brain and peripheral nervous system related to the regulation of visceral activity, and analyzing its complex neural pathways. The brain is the advanced center of visceral function regulation. As an advanced center for substance metabolism and visceral regulation, the hippocampus is crucial for regulating visceral function. The liver is the core organ of material metabolism, and its afferent signals are mainly projected to the Nucleus of the solitary tract(NTS) through vagus nerve, and then they are projected to the hypothalamus and limbic system. Materials and methods: We placed a stereotaxic instrument on the head of each rat and performed craniotomy to open a window above the left hippocampus. We used gold-plated tungsten electrodes to monitor hippocampal neuronal discharges. Grounding was achieved using screws and silver wire. We electrically stimulated the liver branch of the vagus nerve and observed changes in hippocampal neuron discharges using a biological method; in this way, we identified hepatosensitive hippocampal region. We injected FluoroGold into this region and related brain areas. After 3 days, the rats were sacrificed and perfused; the hippocampi were fixed, dehydated, frozen, sectioned, and subjected to fluorescence microscopy. Results: Nerve discharge frequency and amplitude significantly increased in the hippocampal CA3 region (AP:-4.9, ML:-5.1, DV:-5.0 mm). After FluoroGold was injected into the left hepatosensitive region in the hippocampus, labeled cells were found in the contralateral hippocampus, ipsilateral piriform cortex (PC), locus coeruleus (LC) and bilateral lateral hypothalamus (LHA); fluorescence in the ipsilateral hypothalamus was stronger than that of the contralateral hypothalamus. FluoroGold was injected into the LHA, PC, and LC; no labeled cells were found in the hippocampal CA3 region or in the control group. Conclusions: The hippocampal CA3 area of rats may contain a hepatosensitive region that plays important roles in the regulation of liver and other organ function. These region may receive input from the LHA, PC, and LC.

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Section: Discussionmentioning

confidence: 99%

Identification of hepatosensitive region and their neural connections in the hippocampus of rats

et al. 2022

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“…Accumulating evidence has shown that the change of biological function of neuronal cells is involved in the development of various brain diseases [62,63]. Moreover, the relationship among neurons and other cells such as microglia and astrocytes is complex in the pathology of brain diseases [64,65].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation

Yue

Zhao

Chen

et al. 2020

J Neuroinflammation

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Background: Accumulating evidence has documented that microRNA-7 (miR-7) plays an important role in the pathology of various diseases. However, the potential role of miR-7 in brain tissue inflammation (BTI) remains unclear. Methods: We detected the expression of miR-7 in LPS-induced murine BTI model and observed the possible effects of miR-7 deficiency on the pathology of BTI. To elucidate the mechanism, the target gene of miR-7 was screened out by Gene chip assay and its potential roles in BTI were evaluated by Western blot, immunofluorescence, and RNAi assay, respectively.Results: MiR-7 was upregulated in brain tissue in BTI mice and its deficiency could significantly aggravate the pathology of brain tissue. Moreover, RORα, a new target molecule of miR-7, was upregulated in brain tissue from miR-7 deficiency BTI mice. Of note, downregulation of RORα could remarkably exacerbate the pathology of brain tissue and elevate the transduction of NF-κB and ERK1/2 signaling pathways in brain tissue from miR-7 deficiency BTI mice. Furthermore, RORα and miR-7 were dominantly co-expressed in neurons of BTI mice. Finally, RORα synergized with miR-7 to control the inflammatory reaction of neuronal cells in response to LPS stimulation.Conclusions: MiR-7 expression is upregulated in BTI model. Moreover, miR-7 synergizes with its target gene RORα to control the inflammation reaction of neurons, thereby orchestrating the pathology of BTI.

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Selective modulation of neuronal firing by pulse stimulations with different frequencies in rat hippocampus

Cited by 2 publications

References 32 publications

Identification of hepatosensitive region and their neural connections in the hippocampus of rats

Identification of hepatosensitive region and their neural connections in the hippocampus of rats

MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation

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