Selective medium for thermophilic campylobacters including Campylobacter upsaliensis.

Aspinall, S T; Wareing, D. R. A.; Hayward, Peter G.; Hutchinson, D. N.

doi:10.1136/jcp.46.9.829

Cited by 56 publications

(55 citation statements)

References 12 publications

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“…Three plating media were used: Columbia blood agar base (Oxoid, CM331) with 5% (v/v) defibrinated horse blood (BA); modified charcoal cefoperazone, deoxycholate agar (mCCDA, Oxoid CM 739 plus SR155); and CAT agar (Aspinall et al (1993) -mCCDA base (Oxoid) plus CAT (cefoperazone, amphotericin, teicoplanin) supplement; Oxoid SR 147E). All were prepared according to the manufacturers' instructions.…”

Section: Isolation Mediamentioning

confidence: 99%

Prevalence of campylobacters and arcobacters in ducks at the abattoir

Ridsdale

Atabay

Corry

1998

Journal of Applied Microbiology

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Ten duck carcasses, five from each of two different flocks, and four pairs of pooled duck caecal contents, each pair from a separate flock, were examined by a variety of techniques for arcobacters and campylobacters. Campylobacter coli, C. jejuni ssp. jejuni, C. upsaliensis, Arcobacter cryaerophilus and A. butzleri were isolated from duck caecal contents. Campylobacter coli, C. jejuni ssp. jejuni, A. cryaerophilus, A. butzleri and A. skirrowii were isolated from carcasses. The most effective methods for isolating these bacteria from carcasses involved selective enrichment in campylobacter enrichment broth, containing a cefoperazone, amphotericin, teicoplanin supplement, followed by plating onto modified charcoal cefoperazone deoxycholate agar (mCCDA), or plating onto non‐selective blood agar after filtration through a 0·65 μm pore size cellulose acetate filter. In contrast, recovery from caecal contents was most effective by direct plating onto mCCDA. API test strips performed poorly, failing to identify A. skirrowii or A. butzleri (which are not included in the scheme), or even many common campylobacters. The Preston biochemical characterization scheme was more helpful, though it did not distinguish between Arcobacter species. The species of most isolates of campylobacter, identified using the Preston scheme, was confirmed by the use of SDS‐PAGE of whole cell proteins and this technique was also used successfully to speciate arcobacters.

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Section: Isolation Mediamentioning

confidence: 99%

Prevalence of campylobacters and arcobacters in ducks at the abattoir

Ridsdale

Atabay

Corry

1998

Journal of Applied Microbiology

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“…The poor growth of C. upsaliensis on modified cefoperazone charcoal deoxycholate (mCCDA), commonly used for the detection of other thermophilic campylobacters (7), has been attributed to its susceptibility to cefoperazone (2). Techniques based on membrane filtration are among the most efficient for isolating C. upsaliensis (1,8,12).…”

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confidence: 99%

“…The prevalence of Campylobacter upsaliensis currently may be underestimated due to the widespread use of unsuitable isolation procedures (2,4). The poor growth of C. upsaliensis on modified cefoperazone charcoal deoxycholate (mCCDA), commonly used for the detection of other thermophilic campylobacters (7), has been attributed to its susceptibility to cefoperazone (2).…”

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Basis of the Superiority of Cefoperazone Amphotericin Teicoplanin for Isolating Campylobacter upsaliensis from Stools

et al. 2001

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The optimum method for isolating Campylobacter upsaliensis from stools has not been clearly defined. In a preliminary study, cefoperazone amphotericin teicoplanin (CAT) selective medium isolated six C. upsaliensis strains which were not detected using modified cefoperazone charcoal deoxycholate (mCCDA). In order to identify the factors that underlie the superiority of CAT over mCCDA for isolating C. upsaliensis, we examined the effect of incubation time and antibiotic content of culture media on the growth of C. upsaliensis isolates using semiquantitative methods. The recovery of a subgroup of C. upsaliensis isolates from seeded stool specimens was also evaluated. Differences in growth of C. upsaliensis on CAT and mCCDA were modest and were not explained by the antibiotic profiles of the two media. Recovery of C. upsaliensis from spiked human feces on CAT was superior to that on mCCDA at lower concentrations of organisms (10 3 CFU/ml). We conclude that although CAT is more suitable than mCCDA for the isolation of C. upsaliensis from stools, the superiority of CAT for detecting this organism is not accounted for by the antibiotic composition of the medium.

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“…We used as selective agar plates the following media: (i) modified-charcoal cefoperazone deoxycholate agar (mCCDA), a blood-free selective agar base supplemented with 32 g of cefoperazone/ml and 10 g of amphotericin/ml (Oxoid CM 739 with Oxoid supplement SR 155) which is widely used in medical, veterinary, and food microbiology laboratories for the isolation of Campylobacter; (ii) cefoperazone amphotericin teicoplanin selective medium (CAT), a bloodfree charcoal based agar containing 8 g of cefoperazone/ml, 4 g of teicoplanin/ml, and 10 g of amphotericin/ml (Oxoid supplement SR 174); and (iii) Karmali, a blood-free charcoalbased agar containing 32 g of cefazolin/ml, 20 g of vancomycin/ml, and 100 g of cycloheximide/ml (Oxoid supplement SR 167). The latter two types of plates were used because they are recommended for the detection of Campylobacter upsaliensis and Campylobacter helveticus (2,10). All plates were incubated at 37°C under microaerobic conditions in jars (Anoxomat, Mart, Lichtenvoorde, The Netherlands) (5% O 2 , 10% CO 2 , 85% H 2 ) and examined daily for growth for 4 to 6 days.…”

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confidence: 99%