Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

Shafiee, Hadi; Sano, Michael B.; Henslee, Erin A.; Caldwell, John L.; Davalos, Rafael V.

doi:10.1039/b920590j

Cited by 254 publications

(243 citation statements)

References 39 publications

(40 reference statements)

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“…This behaviour of bioparticles forming pearl chains when trapped in DEP devices has been previously reported. 29 At high frequencies (<200 kHz), however, trapping of bacteria was concentrated at the microposts with shorter pearl chains. This is attributed to stronger pDEP forces at high frequencies than at low frequencies, and these therefore pull the entire pearl chain to the 3D insulating microposts, where the electric field gradient is greatest.…”

Section: B Frequency Responsementioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D pDEP) is presented. This technology combines the benefits of electrodebased DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D pDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 lm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D pDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 ll/h and 70% at flow rates as high as 750 ll/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape. V C 2015 AIP Publishing LLC.

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Section: B Frequency Responsementioning

confidence: 99%

Three dimensional passivated-electrode insulator-based dielectrophoresis

et al. 2015

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“…Moreover, it requires only one microstructure material, which makes it amenable for soft lithography. Recent advances in DEP realizations include the development of contactless DEP 7 and 3D carbon electrodes. 8 DEP is well understood for spherical particles 9,10 and excellent models exist to describe DEP of biological cells.…”

Section: Introductionmentioning

confidence: 99%

Tuning direct current streaming dielectrophoresis of proteins

et al. 2012

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Dielectrophoresis (DEP) of biomolecules has large potential to serve as a novel selectivity parameter for bioanalytical methods such as (pre)concentration, fractionation, and separation. However, in contrast to well-characterized biological cells and (nano)particles, the mechanism of protein DEP is poorly understood, limiting bioanalytical applications for proteins. Here, we demonstrate a detailed investigation of factors influencing DEP of diagnostically relevant immunoglobulin G (IgG) molecules using insulator-based DEP (iDEP) under DC conditions. We found that the pH range in which concentration of IgG due to streaming iDEP occurs without aggregate formation matches the pH range suitable for immunoreactions. Numerical simulations of the electrokinetic factors pertaining to DEP streaming in this range further suggested that the protein charge and electroosmotic flow significantly influence iDEP streaming. These predictions are in accordance with the experimentally observed pH-dependent iDEP streaming profiles as well as the determined IgG molecular properties. Moreover, we observed a transition in the streaming behavior caused by a change from positive to negative DEP induced through micelle formation for the first time experimentally, which is in excellent qualitative agreement with numerical simulations. Our study thus relates molecular immunoglobulin properties to observed iDEP, which will be useful for the future development of protein (pre)concentration or separation methods based on DEP.

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“…These include optical traps [12] and electric field methods (dielectrophoresis or DEP) [13]. These methods have numerous applications, although miniaturisation and integration within MEMS-based devices is problematic.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of an acoustic resonator for particle manipulation in air

Devendran

Billson

Hutchins

et al. 2016

Sensors and Actuators B: Chemical

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(2015) Optimisation of an acoustic resonator for particle manipulation in air. Sensors and Actuators B: Chemical, 224 . pp. 529-538. Permanent WRAP URL:http://wrap.warwick.ac.uk/83726 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for-profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. AbstractAn acoustic resonator system has been investigated for the manipulation and entrapment of micronsized particles in air. Careful consideration of the effect of the thickness and properties of the materials used in the design of the resonator was needed to ensure an optimised resonator. This was achieved using both analytical and finite-element modelling, as well as predictions of acoustic attenuation in air as a function of frequency over the 0.8 to 2.0 MHz frequency range. This resulted in a prediction of the likely operational frequency range to obtain particle manipulation. Experimental results are presented to demonstrate good capture of particles as small as 15 µm in diameter.

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Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

Cited by 254 publications

References 39 publications

Three dimensional passivated-electrode insulator-based dielectrophoresis

Three dimensional passivated-electrode insulator-based dielectrophoresis

Tuning direct current streaming dielectrophoresis of proteins

Optimisation of an acoustic resonator for particle manipulation in air

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