Selective Inhibition of Synthesis of Ribosomal Rna in Escherichia Coli by Levorphanol

Abstract

Note added in proof: Since this manuscript was submitted, Kallos and Rizok'2 have obtained direct chemical evidence indicating that the pipsyl as well as the tosyl group is covalently bonded to a serine residue of yCHT.We are indebted to Dr. Allen Gold for the gift of phenylmethanesulfonyl fluoride used to prepare PMS--yCHT in the early stages of this work.

Role of Charge and Hydrophobic Interactions in the Action of Bactericidal/Permeability-increasing Protein of Neutrophils on Gram-negative Bacteria

“…BPI was isolated from rabbit PMN as previously described (1 (5, 6, unpublished observations) indicating that BPI-envelope interactions with all three strains are essentially the same. The bacteria were grown in a triethanolamine-buffered (pH 7.75-7.9) minimal salts medium (7). Stationary phase overnight cultures were transferred to fresh medium (diluted 1:10) and the subcultures were grown to midlogarithmic phase (3-4 h at 370C; 6-10 X 10' bacteria/ ml).…”

Inhibition of Growth and RNA Biosynthesis of Bacillus cereus by Quinacrine

“…Since the effect was more pronounced on ribosomes or rRNA formation than on sRNA, it is also possible that quinacrine produced a 'shift-down' effect. Other drugs, such as dinitrophenol (Simon, van Praag & Aronson, 1966), levorphanol (Simon & van Praag, 1964;Greene & Magasanik, 1967), and azide and cyanide (Nazar & Wong, r 969) have been observed to produce selective inhibition of RNA synthesis, particularly of rRNA, resembling the action of a more limited nutritive growth medium. Recently it was suggested that salicylate also exhibited such an action in B. cereus (Schwartz & Mandel, The normal messenger activity of RNA makes it doubtful that the quinacrine effect was specifically localized at the step of transcription to form mRNA.…”

Separation of sublethal and lethal effects of the bactericidal/permeability increasing protein on Escherichia coli.

“…Escherichia coli J5, a rough UDP-galactose-4-epimerase negative mutant of the smooth strain 011 1-B4, and the smooth strains E. coli ML-35 (i-, y-, z+) (a generous gift of R. Lehrer, Department of Medicine, UCLA Center for the Health Sciences, Los Angeles, CA) and E. coli 09 were grown in triethanolamine buffered (pH 7.7-7.9) minimal salts medium (13). Other rough strains ofE.…”