Selective induction of micronuclei in the rat/mouse colon and liver by 1,2-dimethylhydrazine: a seven-tissue comparative study

Vs, Zhurkov; Сычева, Л. П.; Salamatova, Olga; Vyskubenko, I.F.; Feldt, E.G.; Sherenesheva, N. I.

doi:10.1016/0165-1218(95)00108-5

Cited by 32 publications

(14 citation statements)

References 10 publications

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“…Studies have shown that tumors develop in various regions of the gastrointestinal tract and small bowel when DMH was administered to rats (Zhurkov, 1996). The present study, in the DMH treated carcinoma model a significant decrease in tissue zinc levels and CuZnSOD activity was observed in various parts of the gastrointestinal tract i.e.…”

Section: Discussionsupporting

confidence: 50%

“…Mild histological changes leading to atypical hyperplasia and nuclear enlargement were observed. As reported in earlier studies, the presence of atypical cells in the liver suggests that besides gastrointestinal tumors, the liver may also be a site for abnormalities (Zhurkov, 1996;Inagake,1995). The significant decrease in liver zinc in our studies could be attributed to the histological changes taking place with administration of DMH, which occurs alongside with the progression of dysplasia in the colon.…”

Section: Discussionmentioning

confidence: 62%

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Zinc and Zinc Related Enzymes in Precancerous and Cancerous Tissue in the Colon of Dimethyl Hydrazine Treated Rats

Christudoss¹,

Selvakumar²,

Pulimood³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 62%

Zinc and Zinc Related Enzymes in Precancerous and Cancerous Tissue in the Colon of Dimethyl Hydrazine Treated Rats

Christudoss¹,

Selvakumar²,

Pulimood³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…In this study we also observed that there was no decrease in tissues zinc levels in the liver in DMH treated rats cotreated with aspirin, vitamin C or zinc. As reported in earlier studies the presence of atypical cells in the liver suggests that besides gastrointestinal tumors the liver may also be a site for abnormalities (Zhurkov et al, 1996;Jegatheeswaran et al, 2013). In our study the lesser decrease in tissue zinc can be attributed to the inhibition of histological alterations in the liver mucosa alongside with the maintenance of the colonic architecture.…”

Section: Discussionmentioning

confidence: 48%

Protective Role of Aspirin, Vitamin C, and Zinc and their Effects on Zinc Status in the DMH-Induced Colon Carcinoma Model

Christudoss

Selvakumar²,

Pulimood³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Chemoprotection refers to the use of specific natural or synthetic chemical agents to suppress or prevent the progression to cancer. The purpose of this study is to assess the protective effect of aspirin, vitamin C or zinc in a dimethyl hydrazine (DMH) colon carcinoma model in rats and to investigate the effect of these supplements on changes associated with colonic zinc status. Rats were randomly divided into three groups, group 1 (aspirin), group 2 (vitamin C) and group 3 (zinc), each being subdivided into two groups and given subcutaneous injection of DMH (30 mg/kg body wt) twice a week for 3 months and sacrificed at 4 months (A-precancer model) and 6 months (B-cancer model). Groups 1, 2, 3 were simultaneously given aspirin, vitamin C, or zinc supplement respectively from the beginning till the end of the study. It was observed that 87.5% of rats co-treated with aspirin or vitamin C showed normal colonic histology, along with a significant decrease in colonic tissue zinc at both time points. Rats co-treated with zinc showed 100% reduction in tumor incidence with no significant change in colonic tissue zinc. Plasma zinc, colonic CuZnSOD (copper-zinc superoxide dismutase) and alkaline phosphatase activity showed no significant changes in all 3 cotreated groups. These results suggest that aspirin, vitamin C or zinc given separately, exert a chemoprotective effect against chemically induced DMH colonic preneoplastic progression and colonic carcinogenesis in rats. The inhibitory effects are associated with maintaining the colonic tissue zinc levels and zinc enzymes at near normal without significant changes.

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“…Since Tates et al reported a liver micronucleus assay in 1980, a number of variations have been published (Cliet et al, 1989;Roy and Das, 1990;Mereto et al, 1994;Zhurkov et al, 1996). In these studies, the PH method, the chemical mitogens method, and an in vivo/in vitro method have been applied to assess micronucleus induction in liver cells.…”

Section: Discussionmentioning

confidence: 99%

“…The division of hepatocytes is stimulated by PH, and positive responses were obtained in induction of micronuclei in liver cells after treatment with liver carcinogens that gave negative results in the BM assay. Other investigators also evaluated the chemical clastogenicity potential in the liver by the PH method (Cliet et al, 1989;Roy and Das, 1990;Mere-to et al, 1994;Zhurkov et al, 1996). There are, however, two shortcomings in the PH method: 1) Technically it is not easy to perform successful hepatectomy on all animals used in the assay, and 2) it has been reported that cytochrome P450, styrene mono-oxygenase, epoxide hydrolase, and glutathione-Sepoxide transferase activities decreased by 50, 35, 50, and 35 %, respectively 12 h after PH (Rossi et al, 1987).…”

mentioning

confidence: 99%

A liver micronucleus assay using young rats exposed to diethylnitrosamine: methodological establishment and evaluation

Suzuki¹,

Shirotori

Hayashi

2004

Cytogenet Genome Res

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We have developed a simple liver micronucleus assay using young rats (up to 4 weeks old) to assess cytogenetic damage of chemicals in liver cells. Diethylnitrosamine (DEN) was used as a model rodent hepatocarcinogen in this study. Compared to the partial hepatectomy method most commonly used for the liver micronucleus assay, the present assay method showed equal or even higher practicability. The young rat liver micronucleus assay was also characterized for rat strain differences, sampling time after treatment, single treatment vs. split treatment, age of animals, administration route, and staining method. Although based on one model chemical, we propose an acceptable protocol for the micronucleus assay using young rat liver as follows: Up to 4-week-old rats should be used; oral or intraperitoneal administration can be used; single or repeated treatment protocols can be applied; sampling time is 3–5 days after the last treatment; hepatocytes are prepared by the collagenase perfusion method; and cells are stained with the AO-DAPI double staining method.

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Selective induction of micronuclei in the rat/mouse colon and liver by 1,2-dimethylhydrazine: a seven-tissue comparative study

Cited by 32 publications

References 10 publications

Zinc and Zinc Related Enzymes in Precancerous and Cancerous Tissue in the Colon of Dimethyl Hydrazine Treated Rats

Zinc and Zinc Related Enzymes in Precancerous and Cancerous Tissue in the Colon of Dimethyl Hydrazine Treated Rats

Protective Role of Aspirin, Vitamin C, and Zinc and their Effects on Zinc Status in the DMH-Induced Colon Carcinoma Model

A liver micronucleus assay using young rats exposed to diethylnitrosamine: methodological establishment and evaluation

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