Selective induction of a giant puff in Drosophila hydei by vitamin B6 and derivatives

Leenders, H.J.; Derksen, J.J.L.; Maas, P.; Berendes, H. D.

doi:10.1007/bf00396502

Cited by 34 publications

(15 citation statements)

References 13 publications

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“…The observation that the Q 18 antibody decorates a single heat-shock puff in D. melanogaster (93 D), D. virilis (20 CD) and in D. hydei (48 B), as well as the ultrastructural similarity of these puffs, suggest that they are functionally equivalent. This is also supported by earlier data: all three puffs can be induced independently of the other heat-shock puffs (Lakhotia 1749 Heat-shock puff 93 D of D. melanogaster and Mukherjee, 1970Mukherjee, , 1980Leenders et al, 1973;Bonner and Pardue, 1976;Gubenko and Barisheva, 1979;Lakhotia and Singh, 1982); 93 D and 48 B map close to the ebony locus (D'Alessandro et al, 1977;Henikoff, 1980;Grond et al, 1982) and produce a RNA that remains largely in the nucleus and is unlikely to code for a protein (Lengyel et al, 1980;Lubsen et al, 1978;Peters et al, 1982). These similarities of the described loci are, however, not paralleled by conservation at the level of primary DNA structure (Peters et al, 1980(Peters et al, , 1982.…”

Section: Discussionsupporting

confidence: 88%

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

Dangli¹,

Grond²,

Kloetzel³

et al. 1983

The EMBO Journal

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The monoclonal antibody P11 is directed against a 38 000 dalton protein of Drosophila melanogaster. On polytene chromosomes this protein is present in a subset of the RNA polymerase II‐containing loci. Here we show by density centrifugation and enzyme‐linked immunosorbent assay tests that the P11 antigen is part of nuclear ribonucleoprotein (RNP) complexes. Indirect immunofluorescence shows that, after prolonged heat‐shock, the P11 antigen is present only in the heat‐shock puff 93 D. Identical distribution patterns were obtained with another monoclonal antibody, Q18. Unlike P11, this antibody also cross‐reacts with D. hydei and D. virilis polytene chromosomes, where the puffs 48 B and 20 CD, respectively, are the only loci prominently stained after heat‐shock. The small and giant RNP complexes previously described in these puffs were also observed in puff 93 D. Both types of particle contain the P11 antigen as shown by immunoelectron microscopy. We suggest that the P11 antigen is associated with a special class of RNPs which are possibly involved in the storage of primary transcription products inside the nucleus.

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Section: Discussionsupporting

confidence: 88%

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

Dangli¹,

Grond²,

Kloetzel³

et al. 1983

The EMBO Journal

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“…At the electron microscope level, RNPs are observed that resemble those described in D. yakuba (Thomopoulos and Kastritsis 1979), in species of the takahashii subgroup (unpublished results), in D. hydei after treatment with vitamin B, (Leenders et al 1973;Derksen et al 1973;Derksen 1975Derksen , 1976, and in D. virilis (Swift 1965). Cytochemical studies in D. hydei (Derksen and Willart 1976) have shown that the core of the particles consists mainly of proteins with a relatively poor content in arginine and lysine residues, whereas the surrounding small particles contain both RNA and proteins rich in these residues.…”

Section: Discussionmentioning

confidence: 58%

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

Thomopoulos¹,

Neophytou²,

Kastritsis³

1989

Can. J. Zool.

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Early in the third-instar stage of Drosophila auraria, the salivary gland cells produce small secretory granules of low electron density which empty their flocculent contents into the lumen of the gland. At that stage, the Golgi complexes consist of vesiculated, round cisternae which, as middle third instar is approached, change to their classical appearance. Cytoplasmic protrusions, intramitochondrial granules, and close contacts of mitochondria with lipid droplets are observed during the early developmental stages. At about the middle of the third instar the glue secretory granules start being produced. These secretory granules contain a granular material (where small amounts of vicinal glycol and sulfated groups of complex carbohydrates have been detected) of medium electron density. The glue secretory granules can be classified according to their electron density into two populations. Before exocytosis large amounts of glycogen particles are observed, providing some of the energy needed during the final step of exocytosis. Two different types of ribonucleoprotein particles in puffing sites of the chromosomes are observed. Of these, the larger ones consist of a core surrounded by smaller particles; we suspect that they may be produced in the Balbiani rings observed in the polytene chromosomes of this species. Nuclear blebs and oval bodies are common, particularly during the late developmental stages. The functional significance of these findings during development is discussed.

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“…The unique inducibility of one of the major heat shock puffs in D. mel with benzamide (Lakhotia and Mukherjee, 1970;Mukherjee and Lakhotia, 1982), presence of large RNP particles at this puff (Dangli, et al, 1983) and accumulation of several hnRNPs at this site during heat shock (Saumweber, et al, 1980) have been utilized in early cytogenetic studies to identify an orthologue of 93D or hsrω gene in several species of Drosophila. In D. hyd and related species, the same locus (2-48C) was also known to be selectively induced by brief in vitro exposure of larval salivary glands to vit-B6 or pyridoxal phosphate (Leenders, et al, 1973), although such induction was not seen in D. mel (Lakhotia and Singh, 1982). Table 1 summarizes the earlier cytogenetic evidence for existence of hsrω orthologue in many Drosophila species.…”

Section: Early Cytogenetic Identification Of Hsrω Orthologs In Differmentioning

confidence: 98%

Conservation of gene architecture and domains amidst sequence divergence in thehsrωlncRNA gene across theDrosophilagenus: Anin silicoanalysis

Sahu

Mutt

Lakhotia

2019

Preprint

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The non-coding 93D or hsrω locus in Drosophila melanogaster is important for development and cell stress response, produces multiple long non-coding RNAs using two known transcription start sites and four transcription termination sites and includes two exons, one omega intron, one short translatable open reading frame ORFω, long stretch of tandem repeats unique to this locus and an overlapping miRNA gene near its 3' end. Early cytogenetic and cloning studies suggested its functional conservation in the genus but with little sequence conservation of the ORFω and other regions including tandem repeats while its exon-intron junctions showed ultra-conservation. In this bioinformatic study, we used the landmarks known in Drosophila melanogaster hsrω to map the hsrω orthologue in publicly available annotated and unannotated 34 Drosophila species genomes. This gene is present in all the examined Drosophila species with conserved syntenic neighbours. Sixteen and sixty nucleotide long sequences spanning, respectively, the 5' and 3' splice junctions of the omega intron are ultra-conserved; other regions show varyingly conserved domains. However, the locusspecific tandem repeats are most variable, although a nonamer sequence within them is conserved. Significantly, the ORFω encoding a short alpha helical peptide, with limited sequence conservation, is present in all species indicating its important role. The mir-4951, overlapping with the 3' end of D. melanogaster hsrω, was identified in 33 Drosophila species. Architecture of hsrω lncRNA gene appears more conserved than its base sequence, suggesting that the higher order structures of these lncRNAs are critical for their diverse functions.

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Selective induction of a giant puff in Drosophila hydei by vitamin B6 and derivatives

Cited by 34 publications

References 13 publications

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

An ultrastructural and histochemical developmental study of Drosophila auraria salivary gland cells during the third-instar period

Conservation of gene architecture and domains amidst sequence divergence in thehsrωlncRNA gene across theDrosophilagenus: Anin silicoanalysis

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