Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.

Malinin, George I.

doi:10.1177/24.9.965716

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…When stained with toluidine blue, bisulfite addition compounds of periodate-oxidized sections produce intense metachromasia (Malinin 1970). No metachromatic effect is generated by bisulfite addition compounds in sections oxidized by AA (Malinin 1976). These factors probably account for the more selective deposition of silver in AAoxidized sections than was the case with periodate.…”

Section: Discussionmentioning

confidence: 96%

“…One may recall (Malinin 1976), that besides pyranosides of PGs, the furanosides of nucleic acids are also oxidized by AA. In the case of nuclei, the TCH addition derivatives of oxidized nucleic acids reduce SP and form laminated interference filters.…”

Section: Discussionmentioning

confidence: 98%

“…Semithin and ultrathin cartilage sections were oxidized for 24 h by 30% (v/v) AA in DMSO (Malinin 1976;Lo and Malinin 1989), and then washed with graded solutions of aqueous DMSO. Thereafter, they were immersed in a 1% (w/v) solution of TCH prepared in 10% (v/v) aqueous acetic acid.…”

Section: Deposition Of Silver Gratings On Cartilagementioning

confidence: 99%

“…We have also shown that conversion of cartilage sections into a laminated interference system can be implemented by three tandem reactions. Oxidation of cartilage pyranosides and furanosides to the corresponding carbonyl groups by acetic anhydride (AA) in dimethyl sulfoxide (DMSO) (Malinin 1976) constitutes the first step. Subsequent addition of thiocarbohydrazide (TCH) to cartilage carbonyls (Seligman et al 1965;Thiéry 1967;Thiéry and Rambourg 1974) is the second step.…”

Section: Introductionmentioning

confidence: 99%

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Transmission light microscopy of structurally colored semithin cartilage sections

Malinin

1997

Histochemistry and Cell Biology

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Conversion of osmified tracheal cartilage constituents into an array of laminar interference gratings has been attained by three tandem reactions. Oxidation of semithin, LR white-embedded cartilage sections by acetic anhydride in dimethyl sulfoxide is the first step in the conversion process. Subsequent addition reactions of oxidized cartilage pyranoses and furanoses with thiocarbohydrazide constitutes the second step. Reduction of silver proteinate by thiocarbohydrazones and the concomitant coating of cartilage constituents with silver gratings completes the conversion of cartilage sections into a system of layered interference filters. In transmitted light, all components of converted cartilage display vivid structural colors, which allow detailed microscopic analysis of structurally colored cellular and extracellular cartilage constituents.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Section: Deposition Of Silver Gratings On Cartilagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transmission light microscopy of structurally colored semithin cartilage sections

Malinin

1997

Histochemistry and Cell Biology

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FITC-Dextran tracers in microcirculatory and permeability studies using combined fluorescence stereo microscopy, fluorescence light microscopy and electron microscopy

Thorball

1981

Histochemistry

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Coupling fluorescein-isothiocyanate to dextrans (FITC-D) extends the usefulness of the dextrans as electron microscopic tracer particles by permitting preceding fluorescence stereo microscopy and high-power light microscopy of the tissue specimens. The fate of the tracer may thus be studied in vivo during the experiment, during fixation, and during the succeeding tissue processing. A study of some simple physicochemical characteristics of the tracer, and the influence, if any, of the fixing agent are also made possible. FITC-D was found to be uncharged in the pH range from 6.5 to 8.5, more rapidly precipitated by acetone than by alcohol, and to react with glutaraldehyde and osmium tetroxide in an unknown way during tissue fixation. FITC-D with molecular weights 70,000 and 150,000 showed no signs of diffusion during tissue preparation with the methods reported in the paper, whereas FITC-D 40,000 did so to a slight degree, when the tissue was kept for several days in the fixative vehicle. Securing the preservation of the lower molecular weight FITC-Ds during tissue fixation and preparation is more difficult and the described methods are not adequate. Dextrans provoke an anaphylactic reaction in most rat strains, but are well tolerated by Wistar Furth rats. The introduction of FITC into the dextran molecule might alter the biological reactions, but was also well tolerated by Wistar Furth rats. Combined fluorescence stereo microscopy, fluorescence microscopy of sections, light microscopy of strained sections and electron microscopy made it possible to follow a particular microcirculatory area, selected in vivo, to the final study in the electron microscope.

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Ultrastructural cytochemistry of hepatic lysosomes and their protein components is selectively revealed by the ninhydrin-dimethyl sulfoxide-thiocarbohydrazide-silver proteinate reaction

Malinin

1989

Histochemistry

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Proteins in lysosomal membranes, lysosomes and within the transtubular network are readily accessible for electron microscopic analysis by a new three-step method. Oxidative deamination of tissue-bound amino acids by ninhydrin in aqueous dimethyl sulfoxide and the concomitant formation of corresponding carbonyl groups comprise the first step. The addition reaction of thiocarbohydrazide to tissue-bound carbonyl groups comprises the second step, while the reduction of silver proteinate by tissue-bound thiocarbohydrazones is the final step of this sequential method. Glutaraldehyde-fixed and osmified ultrathin sections of rat liver embedded in LR White were oxidatively deaminated for 24 h by 1% w/v ninhydrin in aqueous 75% v/v dimethyl sulfoxide (DMSO). They were then incubated for 40 min in aqueous 1% w/v thiocarbohydrazide (TCH) and stained for 30 min at 50 degrees C with silver proteinate (SP). The ninhydrin-dimethyl sulfoxide-thiocarbohydrazide-silver proteinate (N-DMSO-TCH-SP) reaction proved to be chemically specific and highly selective for ultrastructural resolution of the internal structure of lysosomes and their protein components. We conclude that the N-DMSO-TCH-SP reaction is the method of choice for cytochemical elucidation of the protein ultrastructure of lysosomes and their enzymatic aggregates.

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Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.

Cited by 7 publications

References 15 publications

Transmission light microscopy of structurally colored semithin cartilage sections

Transmission light microscopy of structurally colored semithin cartilage sections

FITC-Dextran tracers in microcirculatory and permeability studies using combined fluorescence stereo microscopy, fluorescence light microscopy and electron microscopy

Ultrastructural cytochemistry of hepatic lysosomes and their protein components is selectively revealed by the ninhydrin-dimethyl sulfoxide-thiocarbohydrazide-silver proteinate reaction

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