Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium

Merk, Heather L.; Ashrafi, Hamid; Foolad, Majid R.

doi:10.1007/s10681-012-0729-6

Cited by 51 publications

(45 citation statements)

References 43 publications

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“…, Merk and Foolad , Merk et al. ). The strong level of LB resistance in PI 163245 and high h 2 observed through multiple generations and experiments suggest this resistance can be useful for breeding in tomato.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, new LB resistance genes were identified and mapped to chromosomes 1 and 10 in S. pimpinellifolium accession PI 270443, which displays strong LB resistance comparable to Ph‐2 + Ph‐3 combined (Merk et al. ).…”

mentioning

confidence: 99%

“…Resistance to LB has also been reported on chromosome 6 in the wild tomato species Solanum pennellii, although this QTL is linked to the SP allele and it has been suggested this resistance is potentially a result of indeterminate growth habit rather than a true resistance gene (Smart et al 2007). Recently, new LB resistance genes were identified and mapped to chromosomes 1 and 10 in S. pimpinellifolium accession PI 270443, which displays strong LB resistance comparable to Ph-2 + Ph-3 combined (Merk et al 2012).…”

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confidence: 99%

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Genetic analysis of resistance to tomato late blight in Solanum pimpinellifolium accession PI 163245

Ohlson

Foolad

2016

Plant Breeding

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Late blight (LB), caused by Phytophthora infestans, is one of the most devastating diseases of tomato (Solanum lycopersicum) worldwide. Due to the emergence of new and aggressive P. infestans isolates, identifying new genetic resistance to LB is a priority in tomato breeding. Recently, we reported the identification of several Solanum pimpinellifolium accessions with strong LB resistance. In this study, we investigated the utility of resistant accession PI 163245 for tomato breeding by examining heritability (h 2 ) of resistance and the response to selection for resistance. Estimates of h 2 based on F 2 : F 3 and F 3 : F 4 parent : offspring correlation analyses averaged 0.79 and 0.94, respectively, suggesting the heritable nature of LB resistance in PI 163245. Analysis of response to selection for resistance from F 2 to F 4 generations indicated a realized h 2 of 0.63, confirming the utility of this resistance in tomato breeding. Two methods of estimating the minimum number of loci involved indicated the presence of one major resistance locus. Currently, genetic mapping and breeding efforts are underway to further confirm the viability of this accession for improving tomato LB resistance.

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confidence: 99%

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Genetic analysis of resistance to tomato late blight in Solanum pimpinellifolium accession PI 163245

Ohlson

Foolad

2016

Plant Breeding

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“…Another S. pimpinellifolium accession (PI 270443) with strong resistance to tomato LB was identified, which exhibited resistance comparable to Ph‐2 and Ph‐3 resistance genes combined (Foolad, Merk, Ashrafi, & Kinkade, ). A genetic investigation of this accession indicated that its LB resistance was heritable (Merk & Foolad, ) and controlled by two loci located on chromosomes 1 and 10 (Merk, Ashrafi, & Foolad, ). Breeding efforts are underway to transfer this resistance to elite tomato breeding lines and hybrid cultivars (MR Foolad, unpublished).…”

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confidence: 99%

Genetic analysis of late blight resistance in Solanum pimpinellifolium accession PI 270441: Heritability and response to selection

et al. 2018

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Late blight (LB), caused by Phytophthora infestans, is a destructive disease of tomato (Solanum lycopersicum) worldwide. Currently, there are few commercial cultivars of tomato with resistance to LB, and the disease is mainly controlled by heavy use of fungicides. Due to the emergence of fungicide‐resistant pathogen isolates, there is a concerted effort to identify new genetic sources of resistance and breed new resistant cultivars. A recent screening identified several new tomato accessions with strong resistance to LB. Here, we report on the genetic basis of LB resistance in S. pimpinellifolium accession PI 270441, as determined by generation means analysis and analysis of response to selection, using populations derived from crosses with LB‐susceptible breeding line Fla. 8059. Heritability of LB resistance ranged from 0.76 to 0.78, and the minimum number of genes was estimated 1—few. These results suggest that transfer of LB resistance from PI 270441 to the cultivated tomato should be feasible via a traditional backcross breeding approach. Genetic mapping studies are underway to identify molecular markers associated with resistance in this accession.

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“…, Merk et al. ). The search for new late blight resistance in wild tomato species continues, especially in S. pimpinellifolium and S. habrochaites (Brouwer et al.…”

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Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

et al. 2016

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Late blight caused by Phytophthora infestans is one of the most destructive diseases of tomato (Solanum lycopersicum L.) that mainly occurs in cool and wet environments. With the spread of the A2 mating type and new clonal lineages, fewer fungicides provide effective control of the disease, which has increased its worldwide threat. Host resistance could contribute significantly to sustainable disease control. Ph-3 is a race-specific late blight resistance gene commonly used in commercial tomato breeding. Availability of precise and easy to use gene-based markers would facilitate selection. In this study, a Ph-3 on-gene cleaved amplified polymorphic sequence (CAPS) marker, Ph3.gsm/HincII, was developed based on the published gene sequence of Ph-3. The effectiveness of the marker was evaluated along with other published Ph-3 markers using an F 9 recombinant inbred line (RIL) population derived from NC 23E-2 (93) 9 L3708. Markers Ph3.gsm/HincII and TG328/BstNI accurately genotyped the RIL population for Ph-3. In addition, Ph3.gsm/HincII was able to differentiate variable susceptible alleles. This reliable codominant DNA marker would be very useful in marker-assisted selection, particularly for resistance gene pyramiding.

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Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium

Cited by 51 publications

References 43 publications

Genetic analysis of resistance to tomato late blight in Solanum pimpinellifolium accession PI 163245

Genetic analysis of resistance to tomato late blight in Solanum pimpinellifolium accession PI 163245

Genetic analysis of late blight resistance in Solanum pimpinellifolium accession PI 270441: Heritability and response to selection

Evaluation of the Ph‐3 gene‐specific marker developed for marker‐assisted selection of late blight‐resistant tomato

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