Selective Extraction of Cholesterol from Dairy Samples Using a Polypyrrole Molecularly Imprinted Polymer and Determination by Gas Chromatography

Nezhadali, Azizollah; Es’haghi, Zarrin; Khatibi, Aram-Dokht

doi:10.1007/s12161-016-0686-0

Cited by 10 publications

(4 citation statements)

References 58 publications

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“…By means of FT‐IR, it was possible to obtain information on the chemical composition of the material, which was in accordance with the literature . A characteristic band at 3558 cm −1 (assigned to the O–H stretch mode), at 3000 cm −1 (assigned to the C–H stretching mode with sp 3 hybridization), at 1729 cm −1 (attributed to the stretch mode C = O), at 1649 cm −1 (characteristic carbon with sp 2 hybridization), which was believed to be from unsaturation in the reagents that have not been consumed and between 1264 and 1160 cm −1 (assigned to the C–O stretching mode), were common to both organic MIP and organic NIP, as shown in Fig.…”

Section: Resultssupporting

confidence: 74%

“…The pore structure is too small to be clearly visible by this technique. This morphological profile is very common for polymers synthesized by the method in bulk [26,[31][32][33]. Moreover, its apparently porous morphology is ideal for the adsorption of the template molecule.…”

Section: Semmentioning

confidence: 99%

“…Puoci et al [32] synthesized a sorbent for MISPE to detect CHO in food matrices. Nezhadali et al [33] synthesized a novel MIP based in conducting polymer prepared by chemical oxidation of pyrrole through applying FeCl3 and used as a sorbent for selective batch extraction of CHO in dairy samples.…”

mentioning

confidence: 99%

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Molecularly imprinted solid‐phase extraction coupled with LC–APCI–MS–MS for the selective determination of serum cholesterol

2017

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The assessment of selective molecularly imprinted solid-phase extraction (MISPE) for clean-up and extraction of cholesterol (CHO) from bovine and human serum samples was evaluated. Two molecular imprinted polymers (MIP) based on organic and inorganic/organic (hybrid) materials were obtained and compared regarding recovery and selectivity toward CHO. Optimum chromatographic separation between the CHO and its isobaric compound lathosterol (LATHO) has been achieved within 15 min using an Agilent Poroshell 120 EC-C18 column (120 mm × 3.0 mm, 2.7 μM) and mobile phase consisting of acetonitrile:methanol (8:2, v/v) at a flow rate of 0.6 mL min . Mass spectrometry detection was operated in atmospheric pressure chemical ionization (APCI) (+) mode and the SIM transitions [M - H O + H] for CHO and LATHO were 364.1 → 161.2 → 95.2 and 364.1 → 95.1 → 81.1 m/z, respectively. The sample preparation could be accomplished within 1 h, and the matrix effect on the MS signal after sample pretreatment did not interfere in the analyses. The method provided a good linear range (0.013-312 μM.) with coefficients of determination of R > 0.99, substantial sensitivity (limits of detection of 6.5 nM), good recovery (∼90%), high reproducibility (intraday and interday relative standard deviations less than 15%) and satisfactory accuracy (relative error ranged from -13.46% to 11.54%). The organic MIP showed superior performance compared with the hybrid. Finally, the MISPE procedure followed by LC-APCI-MS-MS analysis showed their suitable applicability to examine CHO and LATHO in serum samples.

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Section: Resultssupporting

confidence: 74%

Section: Semmentioning

confidence: 99%

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Molecularly imprinted solid‐phase extraction coupled with LC–APCI–MS–MS for the selective determination of serum cholesterol

2017

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“…Molecular imprinting is a promising technique widely used in the synthesis of molecularly imprinted polymers (MIPs) with specific molecular recognition properties [ 86 , 87 ]. Owing to their excellent properties, ILs have been utilized as the functional monomers for preparation of MIPs [ 88 ]. Generally speaking, IL-based MIPs involve multiple interactions between the analyte and ILs (i.e., π-stacking, electrostatic, hydrophobic interactions, and hydrogen bonding) [ 89 , 90 , 91 ].…”

Section: Extraction Of Natural Products Using Ionic Liquidsmentioning

confidence: 99%

Multi-Interactions in Ionic Liquids for Natural Product Extraction

2020

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Natural products with a variety of pharmacological effects are important sources for commercial drugs, and it is very crucial to develop effective techniques to selectively extract and isolate bioactive natural components from the plants against the background of sustainable development. Ionic liquids (ILs) are a kind of designable material with unique physicochemical properties, including good thermal stability, negligible vapor pressure, good solvation ability, etc. ILs have already been used in pharmaceuticals for extraction, purification, drug delivery, etc. It has been reported that multi-interactions, like hydrogen bonding, hydrophobic interactions, play important roles in the extraction of bioactive components from the plants. In this review, recent progress in the understanding of scientific essence of hydrogen bonding, the special interaction, in ILs was summarized. The extraction of various natural products, one important area in pharmaceutical, by conventional and functional ILs as well as the specific roles of multi-interactions in this process were also reviewed. Moreover, problems existing in bioactive compound extraction by ILs and the future developing trends of this area are given, which might be helpful for scientists, especially beginners, in this field.

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Phenylalanine functionalized cryogels for selective cholesterol removal from milk

Göktürk,

Saylan,

Yılmaz

et al. 2024

Chem. Pap.

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Selective Extraction of Cholesterol from Dairy Samples Using a Polypyrrole Molecularly Imprinted Polymer and Determination by Gas Chromatography

Cited by 10 publications

References 58 publications

Molecularly imprinted solid‐phase extraction coupled with LC–APCI–MS–MS for the selective determination of serum cholesterol

Molecularly imprinted solid‐phase extraction coupled with LC–APCI–MS–MS for the selective determination of serum cholesterol

Multi-Interactions in Ionic Liquids for Natural Product Extraction

Phenylalanine functionalized cryogels for selective cholesterol removal from milk

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