“…Notably, these interneurons show unique electrophysiological properties associated with high energy demand ( Kann, 2016 ). To further examine the cellular mechanisms through which lactate disturbs fast network oscillations, we performed single and paired patch-clamp recordings in excitatory pyramidal cells and fast-spiking, inhibitory interneurons ( Figure 6 A) in two regions of the hippocampus, i.e., CA3 and CA1 ( Rozov et al., 2001 ; Valiullina et al., 2017 ). Figure 6 Lactate-Evoked Attenuation of Synaptic Transmission Paired patch-clamp recordings were done in CA3 and CA1 in ex vivo slices.…”
Section: Resultsmentioning
confidence: 99%
“…Notably, these (Kann, 2016). To further examine the cellular mechanisms through which lactate disturbs fast network oscillations, we performed single and paired patch-clamp recordings in excitatory pyramidal cells and fast-spiking, inhibitory interneurons (Figure 6A) in two regions of the hippocampus, i.e., CA3 and CA1 (Rozov et al, 2001;Valiullina et al, 2017).…”
Section: Lactate Attenuates Excitatory and Inhibitory Synaptic Transmissionmentioning
confidence: 99%
“…Trains were delivered with an interval of 10 s. For recordings of excitatory postsynaptic currents (EPSCs), interneurons were held at -70 mV. To evoke synaptic currents extracellularly, theta glass electrodes filled with aCSF (Valiullina et al, 2017) were placed in stratum radiatum within ∼50 -100 µm from the soma of the recorded neuron. Inhibitory synaptic transmission during recordings was blocked by gabazine (10 µM) co-applied with the aCSF.…”
Section: Electrophysiologymentioning
confidence: 99%
“…Glutamatergic synaptic transmission and possible activation of GABAB receptors were blocked by application of CNQX (10 µM), D-AP5 (100 µM) and CGP 54626 (1 µM). Release from CB1-positive perisomatic terminals was abolished by bath application of CB1 receptor agonist CP 55,940 (2 µM) (Valiullina et al, 2017). To determine evoked GABA release in the presence of glucose or lactate, we measured the effect of the low-affinity competitive GABAA receptor antagonist TPMPA (Jones et al, 2001), on the amplitudes of IPSCs evoked by electrical stimulation (10 Hz and 40 Hz).…”
Section: Electrophysiologymentioning
confidence: 99%
“…Analysis of patch-clamp recordings was performed using IGOR PRO (WaveMetrics Inc., Portland, OR, USA) and Sigmaplot (Systat Software Inc., San Jose, CA, USA) (Rozov et al, 2001;Watanabe et al, 2005;Valiullina et al, 2017).…”
Summary
Lactate shuttled from blood, astrocytes, and/or oligodendrocytes may serve as the major glucose alternative in brain energy metabolism. However, its effectiveness in fueling neuronal information processing underlying complex cortex functions like perception and memory is unclear. We show that sole lactate disturbs electrical gamma and theta-gamma oscillations in hippocampal networks by either attenuation or neural bursts. Bursting is suppressed by elevating the glucose fraction in substrate supply. By contrast, lactate does not affect electrical sharp wave-ripple activity featuring lower energy use. Lactate increases the oxygen consumption during the network states, reflecting enhanced oxidative ATP synthesis in mitochondria. Finally, lactate attenuates synaptic transmission in excitatory pyramidal cells and fast-spiking, inhibitory interneurons by reduced neurotransmitter release from presynaptic terminals, whereas action potential generation in the axon is regular. In conclusion, sole lactate is less effective and potentially harmful during gamma-band rhythms by omitting obligatory ATP delivery through fast glycolysis at the synapse.
“…Notably, these interneurons show unique electrophysiological properties associated with high energy demand ( Kann, 2016 ). To further examine the cellular mechanisms through which lactate disturbs fast network oscillations, we performed single and paired patch-clamp recordings in excitatory pyramidal cells and fast-spiking, inhibitory interneurons ( Figure 6 A) in two regions of the hippocampus, i.e., CA3 and CA1 ( Rozov et al., 2001 ; Valiullina et al., 2017 ). Figure 6 Lactate-Evoked Attenuation of Synaptic Transmission Paired patch-clamp recordings were done in CA3 and CA1 in ex vivo slices.…”
Section: Resultsmentioning
confidence: 99%
“…Notably, these (Kann, 2016). To further examine the cellular mechanisms through which lactate disturbs fast network oscillations, we performed single and paired patch-clamp recordings in excitatory pyramidal cells and fast-spiking, inhibitory interneurons (Figure 6A) in two regions of the hippocampus, i.e., CA3 and CA1 (Rozov et al, 2001;Valiullina et al, 2017).…”
Section: Lactate Attenuates Excitatory and Inhibitory Synaptic Transmissionmentioning
confidence: 99%
“…Trains were delivered with an interval of 10 s. For recordings of excitatory postsynaptic currents (EPSCs), interneurons were held at -70 mV. To evoke synaptic currents extracellularly, theta glass electrodes filled with aCSF (Valiullina et al, 2017) were placed in stratum radiatum within ∼50 -100 µm from the soma of the recorded neuron. Inhibitory synaptic transmission during recordings was blocked by gabazine (10 µM) co-applied with the aCSF.…”
Section: Electrophysiologymentioning
confidence: 99%
“…Glutamatergic synaptic transmission and possible activation of GABAB receptors were blocked by application of CNQX (10 µM), D-AP5 (100 µM) and CGP 54626 (1 µM). Release from CB1-positive perisomatic terminals was abolished by bath application of CB1 receptor agonist CP 55,940 (2 µM) (Valiullina et al, 2017). To determine evoked GABA release in the presence of glucose or lactate, we measured the effect of the low-affinity competitive GABAA receptor antagonist TPMPA (Jones et al, 2001), on the amplitudes of IPSCs evoked by electrical stimulation (10 Hz and 40 Hz).…”
Section: Electrophysiologymentioning
confidence: 99%
“…Analysis of patch-clamp recordings was performed using IGOR PRO (WaveMetrics Inc., Portland, OR, USA) and Sigmaplot (Systat Software Inc., San Jose, CA, USA) (Rozov et al, 2001;Watanabe et al, 2005;Valiullina et al, 2017).…”
Summary
Lactate shuttled from blood, astrocytes, and/or oligodendrocytes may serve as the major glucose alternative in brain energy metabolism. However, its effectiveness in fueling neuronal information processing underlying complex cortex functions like perception and memory is unclear. We show that sole lactate disturbs electrical gamma and theta-gamma oscillations in hippocampal networks by either attenuation or neural bursts. Bursting is suppressed by elevating the glucose fraction in substrate supply. By contrast, lactate does not affect electrical sharp wave-ripple activity featuring lower energy use. Lactate increases the oxygen consumption during the network states, reflecting enhanced oxidative ATP synthesis in mitochondria. Finally, lactate attenuates synaptic transmission in excitatory pyramidal cells and fast-spiking, inhibitory interneurons by reduced neurotransmitter release from presynaptic terminals, whereas action potential generation in the axon is regular. In conclusion, sole lactate is less effective and potentially harmful during gamma-band rhythms by omitting obligatory ATP delivery through fast glycolysis at the synapse.
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