Selective Expression of Antibody Classes and Contact Sensitivity Affected by Genes in the Major Histocompatibility Complex

Thomas, Wayne R.; Watkins, M C; Asherson, G. L.

doi:10.1111/j.1365-3083.1979.tb02702.x

Cited by 5 publications

(5 citation statements)

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“…In mice, anti‐DNCB IgA has also been detected , and kinetics studies have shown that measurable levels of IgG are not observed until some 8 days after the initiation of exposure , or after repeat exposure to allergen some 10 days later . Consistent with the early reports of haemagglutinating antibodies , skin sensitization with oxazolone also induces a hapten‐specific IgG response in mice, as measured by ELISA . In those murine studies where the isotype of the IgG response has been characterized, detectable anti‐hapten IgG1, IgG2a and IgG2b production has been recorded , with several reports of a preferential IgG2a response to both DNCB and oxazolone , consistent with selective Th1 cell activation.…”

Section: Anti‐hapten Antibody Responses Induced Following Exposure Tosupporting

confidence: 83%

“…Some decades later, it was shown that topical treatment of guinea‐pigs with DNCB resulted in a marked increase in the number of germinal centres in skin‐draining lymph nodes, indicative of B cell activation and the production of antibodies . Furthermore, topical application to mice of the experimental skin sensitizer oxazolone or picryl chloride [trinitrochlorobenzene (TNCB)] was shown to stimulate antibody production, detected as a function of the ability of serum to agglutinate haptenated red blood cells (RBCs) , although this effect was somewhat mouse strain‐dependent . However, interpretation of the latter results was hampered by the detection of antibodies in non‐immune sera, suggesting that there was considerable background in the assay, or that natural antibodies are present that are reactive with some haptens .…”

Section: Anti‐hapten Antibody Responses Induced Following Exposure Tomentioning

confidence: 99%

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Anti‐hapten antibodies in response to skin sensitization

et al. 2015

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SummaryWhereas T lymphocyte (T cell) activation is the key event in the acquisition of skin sensitization and subsequent elicitation of allergic contact dermatitis, the humoral component of immune responses to organic contact allergens has received little consideration. There is evidence that, in experimental animals, topical exposure to potent contact allergens is associated with B cell activation and proliferation, and hapten-specific antibody production. However, there is very limited evidence available for anti-hapten antibody responses being induced following topical exposure of humans to contact allergens. Nevertheless, it is important to appreciate that there are almost no negative studies in which evidence for antibody production as the result of skin sensitization has been sought and not found. That is, there is absence of evidence rather than evidence of absence. Furthermore, exposure to chemical respiratory allergens, in which the skin has been implicated as a potential route of sensitization, results in anti-hapten antibody responses. It is proposed that skin sensitization to contact allergens will normally be accompanied by antibody production. The phenomenon is worthy of investigation, as anti-hapten antibodies could potentially influence and/or regulate the induction of skin sensitization. Moreover, such antibodies may provide an informative correlate of the extent to which sensitization has been acquired.Key words: allergic contact dermatitis; antibody response; B cell; hapten; IgG; IgM; skin sensitization.From an immunological perspective, the acquisition of skin sensitization and the elicitation of allergic contact dermatitis are immune and inflammatory responses mediated by antigen (hapten)-specific T cells (1-3). For this reason, it is the activation, differentiation and regulation of T cells upon which attention has been focused, and there are available excellent recent reviews that describe Accepted for publication 21 September 2015the roles played by T cells in skin sensitization and allergic contact dermatitis (4-7).However, at least in animals, contact allergens provoke both humoral and cell-mediated immune responses. Thus, topical encounter with a contact allergen will induce anti-hapten antibody formation in addition to the activation and expansion of responsive T cells (8-10). Given the predominant role played by T cells in skin sensitization, it is perhaps not surprising that anti-hapten antibody responses have received comparatively little attention. This is unfortunate because, in theory, a hapten-specific antibody has the ability to influence both the acquisition of skin sensitization and the subsequent elicitation of allergic reactions. Moreover, it is possible that the vigour and isotype distribution of anti-hapten antibodies induced by exposure to contact allergens

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Section: Anti‐hapten Antibody Responses Induced Following Exposure Tosupporting

confidence: 83%

Section: Anti‐hapten Antibody Responses Induced Following Exposure Tomentioning

confidence: 99%

Anti‐hapten antibodies in response to skin sensitization

et al. 2015

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“…Finally, H-2k mice show good contact sensitivity, IgM, IgG and IgE antibody responses [3] and IFN-y production to PC1, while H-2d mice have a much smaller IgE response and virtually produce no IFN-y [6]. Fig.…”

Section: Discussionmentioning

confidence: 95%

“…On day 14 the mice were boosted by painting with 3 % PCl and they were bled on days 21, 25 and 28 [3].…”

Section: Serum Antibody Assaymentioning

confidence: 99%

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Major histocompatibility complex regulation of interleukin‐5 production in the mouse

Dieli¹,

Sireci²,

Lio³

et al. 1993

Eur J Immunol

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Lymph node cells of CBA (H-2k), but not of BALB/c (H-2d) mice immunized epicutaneously with picryl chloride secrete interleukin (IL)-5 when stimulated with the specific antigen in vitro. The low IL-5 production in BALB/c mice persists when either picryl chloride or the unrelated antigen oxazolone are used, when the amount of antigen in vitro is varied and when a secondary response is studied. The difference in IL-5 production maps to the major histocompatibility complex (MHC) in the congenic BALB/b, BALB/c and BALB/k mice. Furthermore, lymph node cells from (k x d) F1 mice produce IL-5 when stimulated by antigen presented on H-2k but not on H-2d antigen-presenting cells. Finally, the low IL-5 production in vitro in BALB/c mice is correlated with low picryl-specific IgA levels in vivo, which otherwise are ten times greater in CBA and BALB/k mice. The influence of MHC on IL-5 production and IgA secretion in the mouse might be a possible basis for the association of MHC with IgA deficiency in humans.

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Major histocompatibility complex regulation of the class of the immune response: the H‐2^d haplotype determines poor interferon‐γ response to several antigens

et al. 1990

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The lymph node cells of CBA (H-2k), but not BALB/c (H-2d) mice, release interferon (IFN)-gamma into the supernatant when immunized with picryl chloride epicutaneously and then exposed to antigen (haptenized cells) in vitro 4 days later. The failure in IFN-gamma production maps to the major histocompatibility complex (MHC; H-2d) in the congenic BALB/c, BALB/k and BALB/b mice. The evidence that this is an MHC regulation of the class of response to a range of antigens and not a classical Ir gene effect is (a) the difference is seen with several antigens including picryl chloride, "oxazolone" and purified protein derivative of tuberculin and (b) BALB/c mice, which fail to produce IFN-gamma, show excellent contact sensitivity to picryl chloride. It was also found that the crosses between responder and nonresponder strains (CBA x BALB/c)F1 respond to antigen on responder cell but not on nonresponder cells. This influence of MHC on the class of the immune response is a possible basis for some of the associations of MHC with disease.

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Selective Expression of Antibody Classes and Contact Sensitivity Affected by Genes in the Major Histocompatibility Complex

Cited by 5 publications

References 26 publications

Anti‐hapten antibodies in response to skin sensitization

Anti‐hapten antibodies in response to skin sensitization

Major histocompatibility complex regulation of interleukin‐5 production in the mouse

Major histocompatibility complex regulation of the class of the immune response: the H‐2^d haplotype determines poor interferon‐γ response to several antigens

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Selective Expression of Antibody Classes and Contact Sensitivity Affected by Genes in the Major Histocompatibility Complex

Cited by 5 publications

References 26 publications

Anti‐hapten antibodies in response to skin sensitization

Anti‐hapten antibodies in response to skin sensitization

Major histocompatibility complex regulation of interleukin‐5 production in the mouse

Major histocompatibility complex regulation of the class of the immune response: the H‐2d haplotype determines poor interferon‐γ response to several antigens

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Major histocompatibility complex regulation of the class of the immune response: the H‐2^d haplotype determines poor interferon‐γ response to several antigens