Selective ethanolysis of sunflower oil with Lipozyme RM IM, an immobilized Rhizomucor miehei lipase, to obtain a biodiesel-like biofuel, which avoids glycerol production through the monoglyceride formation

Calero, Juan; Verdugo, Cristóbal; Luna, Diego; Sancho, Enrique; Luna, Carlos; Posadillo, Alejandro; Bautista, Felipa M.; Romero, Antonio A.

doi:10.1016/j.nbt.2014.02.008

Cited by 55 publications

(45 citation statements)

References 20 publications

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“…To avoid part of these problems, some recombinant 1-3 positional specific lipases have been used, avoiding the presence of glycerol as by-product, obtaining as final product 2-monoglycerides (Calero et al 2014) and minimized the cost of the enzyme due to the production in cell factories (Arnau et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

Bonet-Ragel

López-Pou

Tutusaus

et al. 2017

Biocatalysis and Biotransformation

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The potential application of rice husk ash (RH26) as support for the immobilization of a recombinant Rhizopus oryzae (rROL) lipase as biocatalyst in the enzymatic biodiesel production using alperujo oil and the comparison with commercial hydrophobic support OD403 (RelOD) has been made. Although the specific activity (UA mg support À1 ) was around one-half lower in RH26 than in RelOD when they were used as biocatalyst in biodiesel reaction, the normalized initial rate was similar, between 1.6 and 2.4 lmol FAME mL À1 mL À1 UA À1 . Thus in terms of biocatalysis performance, rice husk as is an alternative to commercial supports. However, the main problem is the more complex recovery of RH26 for the reutilization compared with commercial ones. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

Bonet-Ragel

López-Pou

Tutusaus

et al. 2017

Biocatalysis and Biotransformation

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“…Therefore, the yield achieved in our work is very promising because it represents around 91% of the maximum theoretical yield. This final product, containing around 60 and 30 wt% of FAEE and MAG, respectively (Table ), cannot be called biodiesel, as in its composition will involve 2‐acylglycerols, but similar biofuels have been proposed by other authors, in some cases using PPL . This “Ecofuel” use glycerol in the final formulation, but it may have a reduced application due to the melting temperature.…”

Section: Resultsmentioning

confidence: 93%

1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates

Ramos

Miranda

Giordano

et al. 2018

Biotechnology Progress

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The preparation of crosslinked aggregates of pancreatic porcine lipase (PPL-CLEA) was systematically studied, evaluating the influence of three precipitants and two crosslinking agents, as well as the use of soy protein as an alternative feeder protein on the catalytic properties and stability of the immobilized PPL. Standard CLEAs showed a global yield (CLEA' observed activity/offered total activity) of less than 4%, whereas with the addition of soy protein (PPL:soy protein mass ratio of 1:3) the global yield was approximately fivefold higher. The CLEA of PPL prepared with soy protein as feeder (PPL:soy protein mass ratio of 1:3) and glutaraldehyde as crosslinking reagent (10 μmol of aldehyde groups/mg of total protein) was more active mainly because of the reduced enzyme leaching in the washing step. This CLEA, named PPL-SOY-CLEA, had an immobilization yield around 60% and an expressed activity around 40%. In the ethanolysis of soybean oil, the PPL-SOY-CLEA yielded maximum fatty acid ethyl ester (FAEE) concentration around 12-fold higher than that achieved using soluble PPL (34 h reaction at 30°C, 300 rpm stirring, soybean oil/ethanol molar ratio of 1:5) with an enzyme load around 2-fold lower (very likely due to free enzyme inactivation). The operational stability of the PPL-SOY-CLEA in the ethanolysis of soybean oil in a vortex flow type reactor showed that FAEE yield was higher than 50% during ten reaction cycles of 24 h. This reactor configuration may be an attractive alternative to the conventional stirred reactors for biotransformations in industrial plants using carrier-free biocatalysts. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:910-920, 2018.

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“…It has a chloroform-like smell and a spicy taste and it is An important handicap of this procedure was the very early verification of the economic difficulty of carrying out this process on an industrial scale, due to the high price of PPL lipases, even with their use in immobilized form, which allows their reuse. Therefore, in the last decade, a high number of low-cost lipases, both in free as well as immobilized forms have been evaluated, demonstrating in all cases the technical efficiency of the process [272][273][274][275][276][277][278][279][280][281][282][283]. However, the economic cost of the process is still the main difficulty to guarantee the economic viability at high production level.…”

Section: Biodiesel-like Biofuels That Integrate Glycerol As Monoglycementioning

confidence: 99%

Biodiesel at the Crossroads: A Critical Review

et al. 2019

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The delay in the energy transition, focused in the replacement of fossil diesel with biodiesel, is mainly caused by the need of reducing the costs associated to the transesterification reaction of vegetable oils with methanol. This reaction, on an industrial scale, presents several problems associated with the glycerol generated during the process. The costs to eliminate this glycerol have to be added to the implicit cost of using seed oil as raw material. Recently, several alternative methods to convert vegetable oils into high quality diesel fuels, which avoid the glycerol generation, are being under development, such as Gliperol, DMC-Biod, or Ecodiesel. Besides, there are renewable diesel fuels known as “green diesel”, obtained by several catalytic processes (cracking or pyrolysis, hydrodeoxygenation and hydrotreating) of vegetable oils and which exhibit a lot of similarities with fossil fuels. Likewise, it has also been addressed as a novel strategy, the use of straight vegetable oils in blends with various plant-based sources such as alcohols, vegetable oils, and several organic compounds that are renewable and biodegradable. These plant-based sources are capable of achieving the effective reduction of the viscosity of the blends, allowing their use in combustion ignition engines. The aim of this review is to evaluate the real possibilities that conventional biodiesel has in order to success as the main biofuel for the energy transition, as well as the use of alternative biofuels that can take part in the energy transition in a successful way.

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Selective ethanolysis of sunflower oil with Lipozyme RM IM, an immobilized Rhizomucor miehei lipase, to obtain a biodiesel-like biofuel, which avoids glycerol production through the monoglyceride formation

Cited by 55 publications

References 20 publications

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

Rice husk ash as a potential carrier for the immobilization of lipases applied in the enzymatic production of biodiesel

1,3‐Regiospecific ethanolysis of soybean oil catalyzed by crosslinked porcine pancreas lipase aggregates

Biodiesel at the Crossroads: A Critical Review

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