Selective entrainment of the Drosophilacircadian clock to daily gradients in environmental temperature

Currie, Jake; Goda, Tadahiro; Wijnen, Herman

doi:10.1186/1741-7007-7-49

Cited by 47 publications

(59 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intact flies entrain both molecular and behavioural circadian rhythms to environmental temperature cycles [2][3][4][5][6][7]. Moreover, temperature-dependent rhythms in clock gene-luciferase reporter constructs persist in cultures of dissected tissues [5,6,8], indicating that relevant temperature sensors are distributed throughout the fly.…”

Section: Introductionmentioning

confidence: 99%

“…Previously published protocols [2,4] were adjusted to accommodate use of a LI-COR Odyssey infrared imaging system for quantitative analysis. In particular, Immobilon-FL PVDF membrane Odyssey-blocking buffer and IRDye-conjugated secondary antibodies (used at 1/10 000 dilution) were substituted for nitrocellulose membrane, 5% non-fat dry milk 1Â TBST blocking buffer and horseradish peroxidase-linked secondary antibodies, respectively.…”

Section: (B) Locomotor Activity Analysesmentioning

confidence: 99%

“…Adult flies were assayed for locomotor behaviour as described previously [4,40]. The transition day counts used in the electronic supplementary material, figure S1 were interpolated from individual actograms.…”

Section: (B) Locomotor Activity Analysesmentioning

confidence: 99%

See 2 more Smart Citations

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

2014

Self Cite

View full text Add to dashboard Cite

Circadian clocks responsible for daily time keeping in a wide range of organisms synchronize to daily temperature cycles via pathways that remain poorly understood. To address this problem from the perspective of the molecular oscillator, we monitored temperature-dependent resetting of four of its core components in the fruitfly Drosophila melanogaster: the transcripts and proteins for the clock genes period ( per) and timeless (tim). The molecular circadian cycle in adult heads exhibited parallel responses to temperature-mediated resetting at the levels of per transcript, tim transcript and TIM protein. Early phase adjustment specific to per transcript rhythms was explained by clockindependent temperature-driven transcription of per. The cold-induced expression of Drosophila per contrasts with the previously reported heatinduced regulation of mammalian Period 2. An altered and more readily reentrainable temperature-synchronized circadian oscillator that featured temperature-driven per transcript rhythms and phase-shifted TIM and PER protein rhythms was found for flies of the 'Tim 4' genotype, which lacked daily tim transcript oscillations but maintained post-transcriptional temperature entrainment of tim expression. The accelerated molecular and behavioural temperature entrainment observed for Tim 4 flies indicates that clock-controlled tim expression constrains the rate of temperature cycle-mediated circadian resetting.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: (B) Locomotor Activity Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the 'fly in the ointment' is that per 01 mutants show essentially the same phenotype as the wild-type in terms of their natural siesta, so this would argue that the mechanism for generating the siesta may be dependent on per splicing (at least at high temperatures), but, oddly, independent of the PER protein. A further relevant laboratory study that introduced natural temperature ranges into the entrainment procedure also observed that behavioural phase changes in colder conditions could be generated as expected, even in the splice-locked per mutants [55]. This would suggest that under more natural thermal ranges, per splicing may not be the only contributor to behavioural phase changes.…”

Section: Alternative Splicing Mediates Clock Responses To the Environmentioning

confidence: 76%

Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs

et al. 2013

View full text Add to dashboard Cite

Circadian (24 h) clocks provide a source of internal timing in most living organisms. These clocks keep time by using complex transcriptional/post-translational feedback loops that are strikingly resilient to changes in environmental conditions. In the last few years, interest has increased in the role of post-transcriptional regulation of circadian clock components. Post-transcriptional control plays a prominent role in modulating rapid responses of the circadian system to environmental changes, including light, temperature and general stress and will be the focus of this review.

show abstract

“…The temperatures used for this experiment were chosen for two reasons: (1) both temperatures (27 and 32°C) are known to sustain robust rhythms in A. carolinensis pineal culture (Menaker and Wisner 1983), and (2) these temperatures reflect typical day/night temperature cycles in Puerto Rico, at least during part of the year, although both higher-and lower-amplitude temperature cycles also occur. Recently, it was found that thermal entrainment of locomotor activity rhythms in Drosophila occurs slowly, requiring several days (Currie et al 2009), in contrast to the rapid resetting (in response to 6-h temperature pulses) observed in chick pineal (Barrett and Takahashi 1995). It has been proposed that, in ectotherms, gradual entrainment to temperature cues would be advantageous in nature, as it would ensure that phase changes are not caused by ''anecdotal'' fluctuations in temperature (Currie et al 2009).…”

Section: Discussionmentioning

confidence: 96%

Photic resetting of the circadian clock is correlated with photic habitat in Anolis lizards

Moore

Menaker

2012

J Comp Physiol A

View full text Add to dashboard Cite

Circadian rhythms are regulated by an internal clock, which is itself synchronized to environmental cues such as light and temperature. It is widely assumed that the circadian system is adapted to local cues, which vary enormously across habitats, yet the comparative data necessary for testing this idea are lacking. We examined photic and thermal resetting of the circadian clock in five species of Anolis lizards whose microhabitats differ in the amounts of sun and shade. The primary circadian oscillator in Anolis is the pineal gland, which produces the hormone melatonin. A flow-through culture system was employed to measure rhythmic melatonin output from individually cultured pineal glands. All species showed temperature-compensated circadian rhythms of pineal melatonin. Light caused significant phase delays of the melatonin rhythm, and this effect varied among species. Controlling for phylogenetic differences, the results indicate that the pineal glands of shade-dwelling species are more sensitive to photic resetting than species living in more brightly illuminated habitats. The differences were not due to variation in free-running period, but may be due to variation in oscillator phase and/or robustness. Surprisingly, thermal resetting was not statistically significant. Overall, the results suggest that the Anolis circadian system is adapted to photic habitat.

show abstract

Selective entrainment of the Drosophilacircadian clock to daily gradients in environmental temperature

Cited by 47 publications

References 39 publications

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

Temperature-dependent resetting of the molecular circadian oscillator inDrosophila

Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs

Photic resetting of the circadian clock is correlated with photic habitat in Anolis lizards

Contact Info

Product

Resources

About