Selective enrichment of bacterial pathogens by microplastic biofilm

Xin-rong, WU; Pan, Jie; Li, Meng; Yao, Li; Bartlam, Mark; Wang, Yingying

doi:10.1016/j.watres.2019.114979

Cited by 485 publications

(207 citation statements)

References 72 publications

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“…The search was limited to studies that fit the following criteria: (i) were published between 2010 and 2019; (ii) had original data (iii) characterize the biofilm formed on nonbiodegradable plastics; and (iv) use Illumina NGS. This resulted in 50 studies for inclusion in this meta-analysis ( Supplementary Table S1B); 41 studies that characterized only the 16S rRNA gene [25, 27-33, 35, 38, 41, 42, 51-79], two studies that characterized only the 18S rRNA gene [34,80], two studies that characterized the 16S and 18S rRNA genes [26,81], two studies that characterized the 16S and ITS2 rRNA genes [82,83], two studies that used shotgun metagenomics [84,85], and one study that used shotgun metagenomics and characterized the 16S rRNA gene [86].…”

Section: Experimental Designmentioning

confidence: 99%

“…Requests for raw sequencing data and metadata were made to the corresponding and first authors of the remaining 16 studies, resulting in the provision of these data for a further five studies. This resulted in 39 studies with datasets that were available for inclusion: 31 that characterized only the 16S rRNA gene [25, 27, 28, 30-33, 35, 38, 52, 53, 55-59, 61, 62, 64-66, 68, 69, 71-76, 78, 79], two that characterized only the 18S rRNA gene [34,80], two that characterized the 16S and ITS2 rRNA genes [82,83], two that used shotgun metagenomics [84,85], one that characterized the 16S and 18S rRNA genes [26], and one that used shotgun metagenomics and characterized the 16S rRNA gene [86] (Supplementary Table S1B). Relatively few studies focussed on the 18S and ITS2 rRNA genes and these used primers that targeted different regions and were from different environments.…”

Section: Experimental Designmentioning

confidence: 99%

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Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

2020

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It is now indisputable that plastics are ubiquitous and problematic in ecosystems globally. Many suggestions have been made about the role that biofilms colonizing plastics in the environment—termed the “Plastisphere”—may play in the transportation and ecological impact of these plastics. By collecting and re-analyzing all raw 16S rRNA gene sequencing and metadata from 2,229 samples within 35 studies, we have performed the first meta-analysis of the Plastisphere in marine, freshwater, other aquatic (e.g., brackish or aquaculture) and terrestrial environments. We show that random forest models can be trained to differentiate between groupings of environmental factors as well as aspects of study design, but—crucially—also between plastics when compared with control biofilms and between different plastic types and community successional stages. Our meta-analysis confirms that potentially biodegrading Plastisphere members, the hydrocarbonoclastic Oceanospirillales and Alteromonadales are consistently more abundant in plastic than control biofilm samples across multiple studies and environments. This indicates the predilection of these organisms for plastics and confirms the urgent need for their ability to biodegrade plastics to be comprehensively tested. We also identified key knowledge gaps that should be addressed by future studies.

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

2020

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“…Plastic particles of less than 1 µm in size are also known as nanoplastics (NPs) [9,10]. These chemically inert MPs/NPs pose significant ecological and health concerns [5] because of their environmental persistence [6,11,12], potential ecotoxicity [13,14], and their ability to act as vectors for chemical pollutants [15][16][17] as well as pathogens [18,19].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

Yong

Valiyaveettil

Tang

2020

IJERPH

521

280

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Fragmented or otherwise miniaturized plastic materials in the form of micro- or nanoplastics have been of nagging environmental concern. Perturbation of organismal physiology and behavior by micro- and nanoplastics have been widely documented for marine invertebrates. Some of these effects are also manifested by larger marine vertebrates such as fishes. More recently, possible effects of micro- and nanoplastics on mammalian gut microbiota as well as host cellular and metabolic toxicity have been reported in mouse models. Human exposure to micro- and nanoplastics occurs largely through ingestion, as these are found in food or derived from food packaging, but also in a less well-defined manner though inhalation. The pathophysiological consequences of acute and chronic micro- and nanoplastics exposure in the mammalian system, particularly humans, are yet unclear. In this review, we focus on the recent findings related to the potential toxicity and detrimental effects of micro- and nanoplastics as demonstrated in mouse models as well as human cell lines. The prevailing data suggest that micro- and nanoplastics accumulation in mammalian and human tissues would likely have negative, yet unclear long-term consequences. There is a need for cellular and systemic toxicity due to micro- and nanoplastics to be better illuminated, and the underlying mechanisms defined by further work.

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“…A wide spectrum of pathogenic microorganisms exists and some form biofilms with microplastics in aquatic environments [111]. Freshwater ecosystem analysis has the formation of biofilms on microplastic substrates by a selected grouping of human pathogens utilizing high-throughput sequencing of 16S rRNA that had distinctive community structures [112]. Opportunistic human pathogens such as Pseudomonas monteillii, Pseudomonas mendocina, and a plant pathogen Pseudomonas syringae were detected forming a microplastic biofilm.…”

Section: Human Health and Pathogenicitymentioning

confidence: 99%

“…The three-dimensional complex communities of microbes found in biofilms form on both organic and inorganic substrates that render bacteria more protected from environmental stressors [112]. Biofilms have been demonstrated and characterized for V. vulnificus, V. parahaemolyticus, V. cholerae, V. fischeri, V. harveyi, and V. anguillarum [120].…”

Section: Human Health and Pathogenicitymentioning

confidence: 99%

The Importance of Biofilms to the Fate and Effects of Microplastics

Glaser¹

2020

Bacterial Biofilms

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Microplastics are global pollutants in water media ranging from drinking water to freshwater streams to oceanic pollutant gyres. Besides the obvious appearance involving a scattered presence in the environmental landscape, microplastics are ubiquitous across modern society in products, food, and beginning to have strong economic effects too. Ingestion of microplastics is virtually unavoidable for each of us as we consume food, breathe air, or drink liquids. For example, beer has been found to be contaminated with plastic materials having the dimensions of microand nanoparticles. In the environment, the formation of biofilms on microplastics is widely observed and this can significantly alter properties important to environmental and human health. Significant research has been conducted on the role of biofilms in the fate and effect of microplastics on environmental and human health, with a general message to avoid contact with microplastics in the environment until more complete strategies for cleanup are developed.

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Selective enrichment of bacterial pathogens by microplastic biofilm

Cited by 485 publications

References 72 publications

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

Food or just a free ride? A meta-analysis reveals the global diversity of the Plastisphere

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

The Importance of Biofilms to the Fate and Effects of Microplastics

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