Selective enhancement of resonance light-scattering of gold nanoparticles by glycogen

Xiang, Minghui; Xu, Xiao; Li, Dongxu; Liu, Feng; Li, Na; Li, Kèan

doi:10.1016/j.talanta.2008.05.027

Cited by 25 publications

(21 citation statements)

References 34 publications

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“…Hybrid systems that combine organic motifs with the electromagnetic properties of inorganic nanoparticles hold potential for developing advanced multifaceted devices for sensing and optical devices . Xiang et al reported that immersion of gold nanoparticles, AuNPs, with a diameter of 20 nm in solutions of OG (up to 0.8 µg mL −1 ) led to an increase of the plasmon absorption of the AuNPs from 520 to 532 nm. This enhancement was possibly due to an increased dielectric constant in the environment surrounding the AuNPs, where the AuNPs aggregate on the surface of glycogen.…”

Section: Glycogen As a Building Block Of Nanostructured Materialsmentioning

confidence: 99%

Glycogen as a Building Block for Advanced Biological Materials

2019

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complex drug delivery systems that are challenging to scale up and therefore have limited clinical and commercial translation. Although numerous nanoparticles have been widely developed and used for various applications, including biomedical applications, [2] there is a growing interest in nontoxic and degradable alternatives to first-generation synthetic nanomaterials. Ideally the synthesis of such nanoparticles needs to be cost effective, simple, green, reproducible, scalable, and the constitutive building blocks of the nanoparticles should be nontoxic, functional, biodegradable, and available on a large scale. Naturally occurring nanoparticles could be such a valid alternative. These nanoparticles include intracellular structures, such as magnetosomes [3] and glycogen, and extracellular assemblies such as lipoproteins and viruses. [3] Among these, glycogen is the most abundant and versatile biological nanoparticle, which fulfils the requirements for the fabrication of nanostructured biomaterials. Glycogen is nature's prime nanoparticle that exists in most organisms, from bacteria and archaea to humans, [4,5] as a vital component of the cellular energy machinery. It is a highly branched polysaccharide comprising repeating units of glucose connected by linear α-d-(1,4) glycosidic linkages with α-d-(1,6) branching, structured into roughly spherical nanoparticles that have a high water solubility and molecular weight.The use of polysaccharides as components for advanced materials has been an attractive concept for decades. [6,7] A key motive is that polysaccharides, as a natural biomaterial, are endowed with a level of biodegradability and biocompatibility. In addition, they can be easily modified chemically or biochemically to produce functional derivatives, which are important for various therapeutic applications. [8] The structures of polysaccharides vary considerably but span from linear to highly branched polymers with molecular weights ranging from thousands to millions, composed of mono-or disaccharides, or short-chain oligomers bound together by glycosidic linkages. [9] There is a rich history of research on the use of polysaccharides in a range of therapeutic applications including: i) soft tissue engineering, [10,11] where the materials can be designed to mimic the mechanical properties of the extracellular matrix in the tissue; ii) as drug, protein, or gene delivery systems, [7,12,13] where polysaccharides have a large number of reactive groups [14] that allow for tunable properties [12] such as pH-responsiveness; [15] and iii) as nanoprobes for cellular Biological nanoparticles found in living systems possess distinct molecular architectures and diverse functions. Glycogen is a unique biological polysaccharide nanoparticle fabricated by nature through a bottom-up approach. The biocatalytic synthesis of glycogen has evolved over time to form a nanometer-sized dendrimer-like structure (20-150 nm) with a highly branched surface and a dense core. This makes glycogen markedly different from other natur...

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Section: Glycogen As a Building Block Of Nanostructured Materialsmentioning

confidence: 99%

Glycogen as a Building Block for Advanced Biological Materials

2019

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“…and L. majuscula cyanobacteria as bio-system was con rmed by FTIR analysis (Figure 3). The absorption bands of glycogen molecule were observed at 1,000 to 1,200 cm −1 [19]. The absorption peaks intensity of Au-glycogen in L. majuscula was higher and narrower in bandwidth than those of Cyanothece sp.…”

Section: Resultsmentioning

confidence: 90%

“…The analytical validation of calibration plotting (Figure 5b) was measured by adding standard solutions of glycogen in concentration range of 10-100 µmol L -1 and mixed with 0.277 mmol L -1 of AuNPs solution. The luminescence intensity of SPR* peak of AuNPs was increased by adding a more concentration of glycogen into AuNPs due to glycogen was stabilized the AuNPs excited state [16,19]. The detection limit (LOD) and quantitation limit (LOQ) of the calibration curve of Au-glycogen were observed 0.89 and 2.95 µmol L -1 respectively, with R (correlation convention) was 0.995.…”

Section: The Chemical Stability Of Aunps-glycogen Conjugatedmentioning

confidence: 97%

Cyanobacteria as nanogold Factories: The chemical validation and perspective of the association between gold nanoparticles and cyanobacterial glycogen

Bakir

ElSemary

2020

Preprint

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Background : Glycogen is the cyanobacterial reserve carbohydrate which is currently the focus of many studies. However, quantification of intercellular glycogen needs thorough investigation. The hypothesis is that glycogen can bind to nanogold. This binding can be used as an important tool for the quantification of intracellular glycogen. Methods: Two strains of cyanobacteria were demonstrated to biosynthesise nanogold intracellularly and to bind to cellular glycogen. Then, spherical gold nanoparticles were chemically prepared and tested for binding to the glycogen molecule of cyanobacterial strains; Lyngbya majuscula and Cyanothece sp. via biochemical method. Experimental analyses were conducted to determine the morphological and optical properties of the Au–glycogen hydrocolloids, together with the analysis of the absorption spectra. The luminescence emission of AuNPs that resulted from recombination between electron in excited state HOMO and hole in ground state LUMO of gold nanoparticles according to Mie theory was recorded. The size diameter and shape of AuNPs were measured via scanning electron microscope and dynamic light scattering techniques. The stability of Au-glycogen was studied by the sequential addition of standard solutions of glycogen in the concentration range (10–100 µmol l− 1) into the prepared AuNPs colloidal solution by recording the SPR and luminescence intensity of AuNPs. Results: The color of the cyanobacterial strains turned into purple color that indicated the formation gold nanoparticles inside the cell (intracellularly). To confirm binding between nanogold and glycogen, the absorption spectrum of AuNPs-glycogen showed plasmon band that was centered at 520–540 nm, suggesting that gold nanoparticles were attached to the surface of the glycogen particles. The interaction of the gold nanoparticles with the biopolymer was further confirmed by photoluminescence spectroscopy analysis. The size diameter of the Au-glycogen in both Lyngbya majuscula and Cyanothece sp. were observed to be 41.7 ± 0.2 nm and 80 ± 30 nm, respectively. FTIR analysis showed that the glycogen absorption peak was observed at 1,000 to 1,200 cm− 1 and exhibited an increase corresponding to the increase in glycogen content in both cyanobacteria. In cyclic voltammetry scans, the Au3+/Au0 redox coupling was observed in case of Lyngbya majuscula indicating the formation of AuNPs-glycogen but in Cyanothece sp. the oxidation anodic peak of AuNPs disappeared which indicated that the AuNPs were highly stabilized in Lyngbya majuscula rather than in Cyanothece sp. This may be attributed to the presence of many thiazole peptides in Lyngbya majuscule. The luminescence of AuNPs showed more stability by the addition of gradual concentrations of glycogen and stronger emission of AuNPs as glycogen protected AuNPs agglomeration. The validation method applied to detect the concentration of glycogen was the use of the change in luminescence of AuNPs in correspondence to binding with glycogen. The detection limit (LOD) and quantitation limit (LOQ) were observed to be 0.89 and 2.95 µmol L-1 respectively. Correlation convention (R) was 0.995. The good chemical stability of this colloidal system and the glycogen biomolecules are studied via density functional theory (DFT). The HOMO level of glycogen unit was closed near to LUMO level of Au3+ that supported the bioconversion of Au3+ into AuNPs via glucose units of glycogen. The detection limit (LOD) and quantitation limit (LOQ) were observed to be 0.89 and 2.95 µmol L− 1 respectively, with R (correlation convention) equal to 0.995. Computational calculations such as density functional theory (DFT) was used to confirm the Au-glycogen complex in bio-system. The HOMO level of glycogen unit was closed near to LUMO level of Au3+ that supported the bioconversion of Au3+ into AuNPs via glucose units of glycogen. Conclusion: The associations formed between the gold nanoparticles and glycogen resulted in good chemical stability. The aggregation of the gold nanoparticles is related to the glycogen concentration and has a profound influence on the absorption properties of Au-glycogen systems. The interparticle distance between AuNPs and glycogen molecule induced the shift in the plasmon band.

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“…[9] Analytical procedures applying the RLS method combine the advantages of simplicity, sensitivity and rapidity. [10][11][12][13][14] Since Huang et al first used this technique for analytical purposes, [10] much attention has been paid to the study and determination of nucleic acids, [15][16][17] proteins [18][19][20] and inorganic ions [21][22][23] by use of this method. In recent years, RLS has been widely applied to determine some pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Resonance light scattering study on the interaction between quinidine sulfate and congo red and its analytical application

et al. 2009

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The interaction between quinidine sulfate (QDS) and congo red (CR) was studied using resonance light scattering (RLS) technique, ultraviolet-visual spectrophotometry and fluorimetry. In weak acidic medium, QDS reacts with CR to form a supermolecular complex which results in the enhanced RLS intensity. Some important interacting parameters, such as the solution acidity and CR concentration, salt effect and addition order of the reagents, were investigated and optimized. Under the optimum conditions, it was found that the enhanced RLS intensity was in proportion to the concentration of QDS in the range 0.2-8.4 microg mL(-1). The corresponding detection limit was 12.0 ng mL(-1). The results showed that this new method enabled simple, sensitive and rapid determination of QDS and was used for the determination of QDS in urine and simulated huamn serum samples.

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Selective enhancement of resonance light-scattering of gold nanoparticles by glycogen

Cited by 25 publications

References 34 publications

Glycogen as a Building Block for Advanced Biological Materials

Glycogen as a Building Block for Advanced Biological Materials

Cyanobacteria as nanogold Factories: The chemical validation and perspective of the association between gold nanoparticles and cyanobacterial glycogen

Resonance light scattering study on the interaction between quinidine sulfate and congo red and its analytical application

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