Selective electrochemical determination of glutathione from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis

Zhao, Lingzhi; Zhao, Lei; Miao, Yanqing; Zhang, Chengxiao

doi:10.1016/j.electacta.2016.04.117

Cited by 26 publications

(9 citation statements)

References 49 publications

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“…Because of these, various detection approaches including chromatographic [17] Raman, colorimetric [8,18–22] fluorimetric [23–25] and electrochemical techniques [26–28] have been developed and utilized for the detection of GSH. Among these techniques,…”

Section: Introductionmentioning

confidence: 99%

“…[13,14] Also it involves in wine making process resulting with low concentration in wines and helps to preserve the fruity aroma by keeping the quinones. [15,16] Because of these, various detection approaches including chromatographic [17] Raman, colorimetric [8,[18][19][20][21][22] fluorimetric [23][24][25] and electrochemical techniques [26][27][28] have been developed and utilized for the detection of GSH. Among these techniques, electrochemical methods are widely used for the determination of many molecules since they have advantages like being economical, simple, and accurate.…”

Section: Introductionmentioning

confidence: 99%

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Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material

et al. 2021

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In this study, we developed a centri‐voltammetric electrochemical sensor for glutathione (GSH) detection. For this purpose, N,N′‐2‐octyloxy‐1‐methyl‐2‐oxoethyl)‐(‐2,4‐thiolbutyloxy)‐1‐methyl‐2‐oxoethyl)‐3,4 : 9,10‐perylenetetracarboxyldiimide (PDI‐C4SH) molecule was used as a carrier molecule for the first time. This molecule, interacted with the thiol ends of GSH and formed a disulfide bridge. By this way, PDI‐C4SH improved the accumulation of GSH onto the electrode surface and increased the sensitivity of the detection protocol. All the electrochemical measurements were carried out with differential pulse voltammetry by using a carbon paste electrode. The linear response was obtained in the range of 0.05 mM and 5 mM for GSH concentration (R2=0.9966) while limit of detection value was calculated as 0.017 mM. For the sample application experiments, developed system was applied to the determination of GSH in red wine samples by using the standard addition method, and an acceptable recovery value of 99.03 % was obtained.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material

et al. 2021

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“…The main reason why we are dealing with electrochemical detection above the other methods previously mentioned is that electrochemical detection has the biggest potential next to optical detection methods, in terms of miniaturization, and thus, in its further use in POCT. For that matter, several recently published works have shown this potential, in using electrochemistry for simple POC-like applications on a real samples [ 29 ] or in studies directly focused on biosensors [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

et al. 2018

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Glutathione (γ-glutamyl-cysteinyl-glycine; also known as GSH) is an endogenous antioxidant that plays a crucial role in cell defense mechanisms against oxidative stress. It is thus not surprising that this molecule can serve as a biomarker for oxidative stress monitoring. As capillary blood is a highly accessible target for biomarking, it is a valuable bodily fluid for diagnosing human GSH levels. This study focused on the optimization of GSH measurements from micro volumes of capillary blood prior to using electrochemical detection. The optimization of experimental parameters, including the sample volume and its stability, was performed and evaluated. Moreover, we tested the optimized method as part of a short-term study. The study consisted of examining 10 subjects within 96 h of their consumption of high amounts of antioxidants, attained from a daily dose of 2 g/150 mL of green tea. The subjects’ capillary blood (5 μL) was taken at 0 h, 48 h, and 96 h for subsequent analysis. The short-term supplementation of diet with green tea showed an increase of GSH pool by approximately 38% (between 0 and 48 h) within all subjects.

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“…An alternative that could potentially overcome some of those drawbacks is the use of electrochemical techniques. As glutathione does not present electrochemical response over bare electrode surfaces, all electrochemical methods for its detection rely on modified electrode surfaces or the use of mediators . Lowinsohn et al .…”

Section: Introductionmentioning

confidence: 99%

Ring‐disc Microelectrodes towards Glutathione Electrochemical Detection

Meloni

Bertotti

2016

Electroanalysis

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1Introduction Glutathione( GSH) is one of the most important endogenous anti-oxidants found in cells [1].I ti sr esponsible for maintaining the redox state of both prokaryotic and eukaryotic cells,p reventing cellular damage by oxidative speciess uch as free radicals and reactive oxygen species (ROS).G SH is at ri-peptidei nt he form Glu-Cys-Gli that takes part in severalc ellular processes such as metabolism, differentiation,d etoxification and apoptosis.G lutathione can be converted to glutathione disulfide( GSSG) throughac hemical reaction with reactive oxidative species in the cellular medium, forming an important defense mechanism against oxidative agents [1][2][3].S tudies show that ac hange in the homeostatic concentration of GSH at the cellular level is af actor that can lead to the development of diseases such as cardiovascular diseases,A lzheimersd isease,d iabetes and cancer [ 1,4,5].Glutathione detection has been performed, mostly,b y high performancel iquid chromatography (HPLC) due to its high sensitivitya nd selectivity [6][7][8].D espite its satisfactory performance,H PLC detection presents some drawbacks such as the need of sample preparation,h igh cost and long analysis time.A na lternative thatc ould potentially overcomes ome of those drawbacksi sthe use of electrochemical techniques.A sg lutathione does not present electrochemical responseover bareelectrode surfaces, all electrochemical methodsf or its detectionr ely on modified electrodesurfaces [9][10][11][12][13][14][15][16][17][18][19][20][21][22] or the use of mediators [23,24].L owinsohn et al. reported the use of acetaminophen, ar eadily available and well-studied pharmaceutical compound, as am ediator for the electrochemical detection of glutathione in solutions with physiological pH [25].Theu se of electrochemical techniques for glutathione detection eliminates the need for sample preparation, allowing for "in vivo" detection of this tri-peptide.I nt hat sense,m icroelectrodes are ap owerful toolb ecause they can be fabricated with physically small dimensions and at ime and space resolved electrochemical responsei s generated, allowingt he application of this kind of sensor in single cell analysis [26][27][28][29][30].F or instance,t he advantageous features of microelectrodes have been explored in studies involving the excretion of thiodione,a ne lectroactive conjugate formed in the reactionb etween menadione and glutathione,a tt he single cell level by means of scanning electrochemical microscopy (SECM) [31,32].In the presentp aper wei nvestigate the application of ar ing-disc microelectrode for indirect electrochemical sensing of glutathione using acetaminophen as an electroactive probe.T he proposed approachi sap roof of concept for space-resolved glutathione detection that could be potentially useful for scanninge lectrochemical mMicroscopya pplication in single cell analysis. 2Experimental 2.1 ChemicalsAll the solid reagents were of analytical grade and were used without further purification. Phosphate buffer saline (PBS) pellet...

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Selective electrochemical determination of glutathione from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis

Cited by 26 publications

References 49 publications

Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material

Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

Ring‐disc Microelectrodes towards Glutathione Electrochemical Detection

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Selective electrochemical determination of glutathione from the leakage of intracellular GSH contents in HeLa cells following doxorubicin-induced cell apoptosis

Cited by 26 publications

References 49 publications

Centri‐Voltammetric GSH Detection with PDI‐C4SH as a Carrier Material

Centri‐Voltammetric GSH Detection with PDI‐C4SH as a Carrier Material

Capillary Blood GSH Level Monitoring, Using an Electrochemical Method Adapted for Micro Volumes

Ring‐disc Microelectrodes towards Glutathione Electrochemical Detection

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Product

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Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material

Centri‐Voltammetric GSH Detection with PDI‐C₄SH as a Carrier Material