Selective Electroanalytical Assay for Cysteine at a Boron Doped Diamond Electrode

Nekrassova, Olga; Lawrence, Nathan S.; Compton, Richard G.

doi:10.1002/elan.200302955

Cited by 45 publications

(22 citation statements)

References 53 publications

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“…Therefore, considerable attention has been focused on determination of l-cysteine [2 -33]. Compared to other detection methodologies, electrochemical technique has some inherent advantages such as simplicity, high sensitivity, ease of miniaturization, and relatively low cost [6,7]. However, it is difficult to determine l-cysteine on conventional electrodes due to sluggish electron transfer and requires high overpotentials to attain reasonable sensitivity [8,9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Preparation of Brilliant‐Blue‐Modified Poly(diallyldimethylammonium Chloride) and Nafion‐Coated Glassy Carbon Electrodes and Their Electrocatalytic Behavior Towards Oxygen and L‐Cysteine

Chen

Thangamuthu

2008

Electroanalysis

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Brilliant blue FCF-modified glassy carbon electrodes have been prepared by cycling the Nafion (or poly(diallyldimethylammonium chloride) (PDDAC)) coated electrodes repeatedly 15 cycles in brilliant blue FCF (BB FCF) dye solution. The BB FCF molecules are incorporated into Nafion coating by cycling the film-covered electrode between þ 0.3 to 1.2 V (vs. Ag/AgCl) in pH 1.5 BB FCF solution while PDDAC-coated electrode cycled between 0 to À 1.0 V (vs. Ag/AgCl) in pH 6.5 BB FCF solution to immobilize the dye. Electrostatic interaction between dye molecule and PDDAC was predominant in PDDAC coating whereas immobilization of dye in Nafion film attributed to the combined effect of electrostatic and hydrophobic interactions. The voltammetric features of BB FCF-modified electrodes resemble that of surface-confined redox couples. The peak potentials of BB FCF-incorporated PDDACcoated electrode were shifted to more positive potential region with decreasing pH of contacting solution. BB FCFmodified electrodes showed electrocatalytic activity towards reduction of oxygen and oxidation of l-cysteine with significant decease of overvoltage compared to unmodified electrode. The BB FCF-modified Nafion-coated electrode was tested for its analytical applications toward determination of l-cysteine. The linear range of calibration plot at BB FCF-modified Nafion-coated electrode is 10 to 100 mM, which coincides with l-cysteine levels in biological fluids. Sensitivity and detection limit of the electrode are 111 nA mM À1 and 0.5 mM, respectively.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Preparation of Brilliant‐Blue‐Modified Poly(diallyldimethylammonium Chloride) and Nafion‐Coated Glassy Carbon Electrodes and Their Electrocatalytic Behavior Towards Oxygen and L‐Cysteine

Chen

Thangamuthu

2008

Electroanalysis

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“…Of the biosensor methods available, electrochemical analysis has been shown to have inherent benefits of simplicity, high sensitivity, good selectivity, easy integration, and relatively low cost. [4][5][6] Therefore, different chemically modified electrodes have been developed for biomolecular analysis, environmental monitoring, and analysis of food quality. [7][8][9][10][11] Functional modification of electrode substrates for the construction of electrochemical biosensors having improved analytical performance with respect to other biosensor designs is highly desirable for developing potential device applications.…”

Section: Introductionmentioning

confidence: 99%

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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Abstract:A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 µA mM. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H 2 O 2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.

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“…It is used in medicine and food industries. As a consequence, its oxidation and accurate determination at low potential constitute a valuable task and the search for sensitive and selective methods in these purposes continues unabated [1][2][3][4]. In this context, electrochemical techniques at solid electrodes appear among the favourites, probably because, simultaneously with detection, they provide interesting insights into the redox behaviours of cysteine.…”

Section: Introductionmentioning

confidence: 99%

“…Also associated with this strategy are several other beneficial improvements including the increase in selectivity, the decrease of surface fouling and the decrease of surface oxide formation. Remarkable achievements were obtained recently with boron-doped diamond electrodes [2,7,11] and also with carbon nanotube materials, unmodified or modified with various catalysts [1][2][3]9].…”

Section: Introductionmentioning

confidence: 99%

Multi-vanadium substituted polyoxometalates as efficient electrocatalysts for the oxidation of l-cysteine at low potential on glassy carbon electrodes

Keita

Contant

Mialane

et al. 2006

Electrochemistry Communications

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In search for efficient electrocatalysts for the oxidation of L-cysteine on glassy carbon, multivanadium-substituted polyoxometalates (POMs, for short) were tested. (P 2 V 3 W 15 ). Electrochemistry shows that the sandwich-type POM contains 2 V IV centers and one V V center and must be formulated As 2 V 2 IV VW 18 , in agreement with titration, elemental analysis and magnetic measurements on this element. The two-electron composite wave that features the redox behaviour of the two V IV centers of As 2 V 2 IV VW 18 are very close to each other and remains practically merged whatever the potential scan rate. In addition of this sharp contrast with the behaviours of the two V V centers of P 2 V 2 W 16 or P 2 MoV 2 W 15 , the very slow electron transfer kinetics associated with the second wave of P 2 V 3 W 15 appears also at variance with the case of As 2 V 2 IV VW 18 . All the POMs of this work proved efficient for the oxidation of L-cysteine. Comparison of the present results with those of mono-Vanadium substituted POMs indicates that accumulation of vanadium atoms in the POM framework is beneficial in the electrocatalytic process. In addition, the present work highlights the important influence of the POM structure in the electrocatalytic oxidation of L-cysteine. The remarkable outcome of this work is that the potential for the oxidation of L-cysteine in the presence of the selected POMs has been substantially driven in the negative direction compared to the case of glassy carbon alone, a feature which is associated with faster kinetics.The stability of the systems must also be pointed out.

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Selective Electroanalytical Assay for Cysteine at a Boron Doped Diamond Electrode

Cited by 45 publications

References 53 publications

Electrochemical Preparation of Brilliant‐Blue‐Modified Poly(diallyldimethylammonium Chloride) and Nafion‐Coated Glassy Carbon Electrodes and Their Electrocatalytic Behavior Towards Oxygen and L‐Cysteine

Electrochemical Preparation of Brilliant‐Blue‐Modified Poly(diallyldimethylammonium Chloride) and Nafion‐Coated Glassy Carbon Electrodes and Their Electrocatalytic Behavior Towards Oxygen and L‐Cysteine

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Multi-vanadium substituted polyoxometalates as efficient electrocatalysts for the oxidation of l-cysteine at low potential on glassy carbon electrodes

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