Selective effects of non-thermal atmospheric plasma on triple-negative breast normal and carcinoma cells through different cell signaling pathways

Abstract

Non-thermal atmospheric plasma (NTP) has shown its selective anticancer effects in many types of tumors in vitro and one of the main mechanisms is that the different increase of intracellular ROS in cancer and homologous normal cells. In this study, we report that NTP treatment reduces the proliferation in triple negative breast cancer (TNBC) and normal cell lines. Simultaneously, STAT3 pathway is inhibited by NTP effects. However, it is observed that normal cells MCF10A are more sensitive to ROS toxicity induced by NTP than cancer cells MDA-MB-231. When 5 mM of ROS inhibitor N-acetyl cysteine (NAC) is employed in NTP treatments, the proliferation of normal breast cells MCF10A recovers. Meanwhile, NTP effects remain significant inhibition of MDA-MB-231 cells. Our results further reveal that NTP can induce apoptosis in MDA-MB-231 cells through inhibiting interleukin-6 receptor (IL-6R) pathway. Moreover, the mechanism of NTP anti-cancer selectivity relates to constantly HER2/Akt activation induced by NTP especially in MCF10A cells but not in MDA-MB-231 cells. Therefore, these two different cell signaling pathways induced by NTP treatments in TNBC and homologous normal cells make NTP becoming a potential tool in future therapy.

Control of multidrug-resistant planktonicAcinetobacter baumannii: biocidal efficacy study by atmospheric-pressure air plasma

“…The length and diameter of the needles are 2 cm and 1 mm respectively. Similar types of configuration are also reported by other articles [27][28][29][30][31]. In this study, the six fine needles were driven by a high voltage DC and the bacterial suspension served as the ground electrode.…”

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

“…Consistent with the findings regarding to oxidative stress correlation to cell death with AgNP and CAP continuously mounts the evidence to show that generation of ROS is highly related to the mechanism of AgNP and CAP with the results in current study showed that the cytotoxicity induced by AgNP alone, as well as the combinational treatment AgNP-CAP were efficiently prevented prior NAC treatment up to 10-fold (see Figure 3). The results determine that oxidative stress is responsible for the cytotoxicity of AgNPs and CAP, which is compliant with previous studies portraying the protective effect of NAC when treated with either AgNP or CAP resulting to recovery of proliferative cells [52][53][54][55].…”

Combination therapy of cold atmospheric plasma (CAP) with temozolomide in the treatment of U87MG glioblastoma cells

“…Combined CAP-TMZ treatment causes a reduction in cell viability compared to TMZ. The anti-tumor effect of CAP as a standalone therapy in the treatment of glioblastoma and other cancers has been previously investigated [22][23][24][25][26][27][28][29][30] . Positive treatment outcomes reported by several studies make CAP a promising treatment strategy to be evaluated for enhancing sensitivity to TMZ in glioblastoma.…”