Selective Effects of Bacterial Endotoxins on Various Subpopulations of Lymphoreticular Cells

Peavy, Duane L.; Shands, Joseph W.; Adler, William H.; Smith, Richard T.

doi:10.1093/infdis/128.supplement_1.s91

Cited by 29 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, it is evident that the biological activity of LPS on cells of the immune system is multifaceted and varies with the conditions under which it is employed. The highly preferential mitogenic effect of LPS on B lymphocytes under normal conditions in vitro has been amply documented (3)(4)(5)(6)(7)(8). Moreover, the stimulation by LPS of antibody production in relatively pure B lymphocyte populations in vitro (5, 8 15-17) and in relatively thymusdeprived mice in vivo (10,11,13) has been reported.…”

Section: Failure Of L P S To Completely Substitute For Helper Cell Fumentioning

confidence: 99%

“…T Cells.--The functional mechanism of LPS potentiation of specific helper Tcell function may be considered in at least two ways: (a) LPS-induced increase in the rate of proliferation of T cells; and/or (b) increased efficiency of individual helper T cells in their exertion of regulatory influences on B lymphocytes. Available data indicate that LPS alone does not induce a proliferative response in T cells (3)(4)(5)(6)(7)(8). However, to our knowledge, the possibility of whether or not LPS can appreciably affect antigen-induced T cell proliferation has not been heretofore explored.…”

Section: Failure Of L P S To Completely Substitute For Helper Cell Fumentioning

confidence: 99%

“…Bacterial endotoxin or lipopolysaccharide (LPS) has been a recent focal point of great interest because of its striking biological effects on lymphocytes. This material has been shown to be highly mitogenic for B lymphocytes (3)(4)(5)(6)(7)(8) and to have rather potent adjuvant properties both in vivo (9)(10)(11)(12)(13)(14) and in vitro (5,8,(15)(16)(17). The target cell(s) of its adjuvant effects has not been conclusively established.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Activation of T and B Lymphocytes in Vitro

Armerding

Katz

1974

The Journal of Experimental Medicine

129

View full text Add to dashboard Cite

The elicitation of antibody responses to all but a selected class of antigens involves a series of apparently complex intra-and inter-cellular events (reviewed in reference 1) whose nature has remained largely obscure despite intensive analysis in recent years. Attempts to circumvent much of the obscurity concerning intercellular reactions (i.e. between T and B lymphocytes) have recently involved detailed experimental assessment of the action of various agents on the biological activation events of one or the other lymphocyte class. Most of these studies have focused on the cellular responses to a variety of mitogenic substances utilizing in vitro systems and several sources of relatively pure T or B lymphocyte populations (for review, see reference 2).Furthermore, in experiments on in vitro antibody production, the predominant antigen employed has been the intact heterologous erythrocyte from one or more species.Bacterial endotoxin or lipopolysaccharide (LPS) has been a recent focal point of great interest because of its striking biological effects on lymphocytes. This material has been shown to be highly mitogenic for B lymphocytes (3-8) and to have rather potent adjuvant properties both in vivo (9-14) and in vitro (5,8,(15)(16)(17). The target cell(s) of its adjuvant effects has not been conclusively established. Indeed, there are compelling data indicating that adjnvanticity of LPS reflects its action on B lymphocytes selectively (8, 11-13, 15, 16) and comparably compelling data that LPS has biological effects on T cells as well (9,14). The present studies were undertaken to determine the nature of the effect of LPS on antibody production. We have done this by making comparative studies of the effects of LPS on in vitro primary and secondary antibody responses to soluble hapten-protein conjugates and to particulate and soluble sheep erythrocyte antigens. The results obtained demonstrate that a major biological effect of LPS on antibody production is mediated through its influence on antigenspecific helper T-cell function.

show abstract

Section: Failure Of L P S To Completely Substitute For Helper Cell Fumentioning

confidence: 99%

Section: Failure Of L P S To Completely Substitute For Helper Cell Fumentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Activation of T and B Lymphocytes in Vitro

Armerding

Katz

1974

The Journal of Experimental Medicine

129

View full text Add to dashboard Cite

show abstract

“…accounts for about 10%; the 1-2x lo6 cells assayed as CFUs account for less than 1%. The subpopulation stimulated by LPS can be estimated on the basis of these and other data (Shands et al, 1973) to constitute 10-30% of the total, but it is not certain that this measures the B cell subpopulation. We have, however, correlative evidence that a B cell subpopulation assessed in terms of susceptibility to the cytotoxic effect of heterologous anti-mouse kappa serum constitutes 8 0 -1 0 0~ lo6 cells, i.e.…”

Section: Discussionmentioning

confidence: 99%

The stimulatory effects of bearing primary methylcholanthrene‐induced tumors upon the murine lymphoreticular system

Smith

Konda

1973

Intl Journal of Cancer

View full text Add to dashboard Cite

Susceptibility to LPS mitogenicity, an apparent B-cell function, was increased relatively and absolutely. The B-cell function of anti-SR BC production was also increased. Colonyforming units increased in number, but they were insuficient to account for significant proportions of the spleen cell population. It was concluded that the animal bearing a primary MCA tumor is deficient in neither T nor B cells and that the effect of the tumor upon immune function is stimulatory.In the course of experiments designed to explore further apparent manifestations of immuno deficiency in tumor-bearing animals (Adler el al., 1971b, a model system was developed in which the spleen and lymph nodes of mice bearing transplanted, syngeneic, methylcholanthreneinduced sarcomas were examined for evidence of competence at the cellular level (Smith and Konda, 1972; Konda and Smith, 1973a; Konda et al., 1973b;Forbes et al., 1973). Contrary to expectations, the absolute size of both the T-and B-cell subpopulations was increased in the spleen and lymph nodes. These conclusions were based upon both the assessment of cells bearing marker antigens and the numbers of cells capable of T-and B-cell functions. It could be argued, however, that the transplanted syngeneic tumor has a different effect on lympho-reticular function from that of a primary tumor, particularly in view of evidence that chemical carcinogens may have an immunodepressive effect (Stjernsward, 1966).Immunologic functions in animals developing primary tumors induced by methylcholanthrene were tested, therefore. In this report methylcholanthrene was used to induce tumors in five different mouse strains; the resultant tumors had varying latency periods and immunogenicity. The effect of these primary tumors upon immunologic functions at the cellular level was stimulatory and similar to those evoked by transplanted syngeneic tumors in each circumstance.

show abstract

“…The release of the bacterial endotoxins, lipopolysaccharides, from bacteria invading the tissue is responsible for the initiation of damage to the tissue (KASS and WOLFF 1973). Regarding the physiological role of bacterial Ii po polysaccharides, it is known that they may trigger lymphocyte transformation and induce release of lymphokines (PEAVY et al 1972), which are potent chemotactic factors (WILLOUGHBY 1978). It has been shown that Salmonella typhosa incubated in the presence of leucocyte extracts, trypsin, lysozyme and inflammatory exudates release considerable amounts of bacteriallipopolysaccharides in contrast to the modest release from controls incubated only in phosphate buffered saline.…”

Section: Polyamine Metabolismmentioning

confidence: 99%