“…specific Th17 cells prepared as described previously (24). Briefly, splenic CD4 + T cells from DO11.10/RAG-2 −/− mice were cultured with X-ray-irradiated BALB/c splenocytes in DMEM/F12 medium supplemented with 10% fetal bovine serum, 0.3 mM ovalbumin (OVA) 323-339 peptide, 10 U/ml recombinant IL-2 (BD Bioscience), 10 ng/ml IL-1b, 20 ng/ml IL-6, 1 ng/ml transforming growth factor-b (Peprotech, Rocky Hill, NJ, USA), and 10 ng/ml IL-23 (R&D Systems, Minneapolis, MN, USA), which were added at the start of the culture together with 40 mg/ml anti-IL-4 and 40 mg/ml anti-IFN-g (eBioscience, San Diego, CA, USA).…”