Selective determination of quinones by high-performance liquid chromatography with on-line post column ultraviolet irradiation and peroxyoxalate chemiluminescence detection

Ahmed, Sameh; Fujii, Shuu; Kishikawa, Naoya; Ohba, Yoshihito; Nakashima, Kenichiro; Kuroda, Naotaka

doi:10.1016/j.chroma.2006.07.078

Cited by 31 publications

(33 citation statements)

References 26 publications

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“…The PO-CL is easily combined with HPLC and applied to highly sensitive determination of fluorescent compounds and hydrogen peroxide. Previously, we had reported the determination of quinones by HPLC-PO-CL detection, without addition of hydrogen peroxide and fluorophore, combined with on-line photodegradation reaction [31] and this method was further adopted for the determination of vitamin K homologues [32].…”

Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-L was sufficient. Besides, it can detect accurately the low plasma concentrations.The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7-102.4 % and the inter-and intra-assay RSD values in rat plasma were 2.5-8.9 %. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single dose of DXR.

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Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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“…However, there has been no report on coupling PICL to CE, probably because the light sources (mercury [16][17][18][19][20][21][22][23], xenon [24,25] or halogen lamp [26,27]) in reported PICL are too large in size to be used in CE detection. In addition, the large amount of heat generated from these light sources [19,22,23,26,27] could deteriorate CE separation performance.…”

Section: Introductionmentioning

confidence: 99%

“…However, these conventional CL systems for CE can detect only a limited number of substances, which can be classified into three groups: (i) the reactants of a CL reaction; (ii) the catalyst, enhancer or inhibitor of a CL reaction; and (iii) the catalyst, enhancer or inhibitor of coupling reactions [15]. Recent studies in photo-induced chemiluminescence (PICL) have extended the detectable analyte range and added a new trend in CL research [16][17][18][19][20][21][22][23][24][25][26][27]. Till now, PICL has been applied for the determination of various compounds, such as pesticides [17,18], quinones [19], aromatic compounds [20], organic peroxides [21], chemical oxygen demand [22], saccharides [23] and insecticides [24,25], and it has been successfully coupled to HPLC for the analysis of complex samples [17, 19-21, 25, 26].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies in photo-induced chemiluminescence (PICL) have extended the detectable analyte range and added a new trend in CL research [16][17][18][19][20][21][22][23][24][25][26][27]. Till now, PICL has been applied for the determination of various compounds, such as pesticides [17,18], quinones [19], aromatic compounds [20], organic peroxides [21], chemical oxygen demand [22], saccharides [23] and insecticides [24,25], and it has been successfully coupled to HPLC for the analysis of complex samples [17, 19-21, 25, 26]. However, there has been no report on coupling PICL to CE, probably because the light sources (mercury [16][17][18][19][20][21][22][23], xenon [24,25] or halogen lamp [26,27]) in reported PICL are too large in size to be used in CE detection.…”

Section: Introductionmentioning

confidence: 99%

“…Till now, PICL has been applied for the determination of various compounds, such as pesticides [17,18], quinones [19], aromatic compounds [20], organic peroxides [21], chemical oxygen demand [22], saccharides [23] and insecticides [24,25], and it has been successfully coupled to HPLC for the analysis of complex samples [17, 19-21, 25, 26]. However, there has been no report on coupling PICL to CE, probably because the light sources (mercury [16][17][18][19][20][21][22][23], xenon [24,25] or halogen lamp [26,27]) in reported PICL are too large in size to be used in CE detection. In addition, the large amount of heat generated from these light sources [19,22,23,26,27] could deteriorate CE separation performance.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Light‐emitting‐diode‐induced chemiluminescence detection for capillary electrophoresis

Zhang

et al. 2009

Electrophoresis

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In this work, an LED-induced-chemiluminescence (LED-CL) system was developed to extend the application of CL detection in CE. In the LED-CL, the analyte photooxidizes luminol under the irradiation of LEDs and generates CL. Taking the advantage of the small size nature of LEDs, the constructed photoreactor is greatly miniaturized, and especially suitable as a CE detector. The feasibility of the proposed detector was evaluated by detection of riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) after CE separation. Under the optimized conditions, the LODs for RF, FMN and FAD were 0.007, 0.02 and 0.1 mg/mL, respectively, better than those by UV detection. The RSDs were 3.4, 3.6 and 4.1% for 0.5 mg/mL RF, 2 mg/mL FMN and 5 mg/mL FAD, respectively. The LED-CL detector features low cost, miniaturization, fast response, high sensitivity and good reproducibility.

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Determination of aromatic compounds by high‐performance liquid chromatography with on‐line photoreactor and peroxyoxalate chemiluminescence detection

et al. 2007

Self Cite

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This paper describes a novel high-performance liquid chromatographic (HPLC) method for the determination of aromatic compounds with peroxyoxalate chemiluminescence (PO-CL ) detection following on-line UV irradiation. Aromatic compounds were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide, which was determined via PO-CL detection using a mixture of bis(2,4,6-trichlorophenyl)oxalate (aryloxalate) and 2,4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine (fluorophore) as a post-column CL reagent. Generation of hydrogen peroxide from aromatic compounds was confirmed using a flow injection analysis (FIA) system incorporating an enzyme column reactor immobilized with catalase. The conditions for UV irradiation were optimized using benzene and monosubstituted benzenes (phenol, benzaldehyde, nitrobenzene and N,N-dimethylaniline) by an HPLC system to evaluate the analytical performance of the proposed system. The detection limits for benzene and monosubstituted benzenes were in the range 2.1-124 pmol/injection at signal:noise (S:N) ratio = 3. Monocyclic and polycyclic hydrocarbons were also employed to investigate their CL properties. The possibility of PO-CL detection for a wide variety of aromatic compounds was shown for the first time.

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Selective determination of quinones by high-performance liquid chromatography with on-line post column ultraviolet irradiation and peroxyoxalate chemiluminescence detection

Cited by 31 publications

References 26 publications

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Light‐emitting‐diode‐induced chemiluminescence detection for capillary electrophoresis

Determination of aromatic compounds by high‐performance liquid chromatography with on‐line photoreactor and peroxyoxalate chemiluminescence detection

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