Selective determination of microbial cells by graphite electrode modified with adsorbed 4,4′-bipyridine

Matsunaga, Tadashi; Namba, Yoichi

doi:10.1016/s0003-2670(00)84284-1

Cited by 23 publications

(14 citation statements)

References 6 publications

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“…Recently, it has been reported by the present_ authors' group that microbial cells are electrochemically oxidized on a graphite electrode and that the electron transfer between microbial cells and the electrode is mediated by intracellular CoA [1,2]. Electrochemical oxidation of microbial cells resulted in a decrease of respiratory activity and in cells death [3] when the constant potential (0.74 V vs. SCE) was applied to cells of S. cerevisiae attached to the electrode surface.…”

Section: Introductionmentioning

confidence: 91%

Photoelectrochemical sterilization of microbial cells by semiconductor powders

Matsunaga

Tomoda

Nakajima

et al. 1985

FEMS Microbiology Letters

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1,289

374

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We report the novel concept of photochemical sterilization. Microbial cells were killed photoelectrochemically with semiconductor powder (platinum‐loaded titanium oxide, TiO2/Pt). Coenzyme A, (CoA) in the whole cells was photo‐electrochemically oxidized and, as a result, the respiration of cells was inhibited. Inhibition of respiratory activity caused death of the cells. Lactobacillus acidophilus, Saccharomyces cerevisiae and Escherichia coli (103 cells/ml respectively) were completely sterilized when they were incubated with TiO2/Pt particles under metal halide lamp irradiation for 60–120 min.

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Section: Introductionmentioning

confidence: 91%

Photoelectrochemical sterilization of microbial cells by semiconductor powders

Matsunaga

Tomoda

Nakajima

et al. 1985

FEMS Microbiology Letters

Self Cite

1,289

374

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“…Toxic substances such as free chlorine (9), Pt complex (10), and H202 (12) generated by electrolysis have been shown to be responsible for the sterilization effect. Recently, we have reported (4,5) that microbial cells are electrochemically oxidized on a graphite electrode and that the electron transfer between microbial cells and the electrode is mediated by intracellular coenzyme A (CoA). Electrochemical oxidation of microbial cells resulted in a decrease in respiratory activity and cell death (6) when the constant potential (0.74 V versus the saturated calomel electrode) was applied to Saccharomyces cerevisiae cells attached to the electrode surface.…”

mentioning

confidence: 99%

Continuous-sterilization system that uses photosemiconductor powders

Matsunaga

Tomoda

Nakajima

et al. 1988

Appl Environ Microbiol

366

111

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We report a novel photochemical sterilization system in which Escherichia coli cells were sterilized with photosemiconductor powders (titanium oxide). For sterilization that could be used in practice, it was necessary to separate the TiO2 powders from the cell suspension. Therefore, semiconductor powders were immobilized on acetylcellulose membranes. We constructed a continuous-sterilization system consisting of a TiO2immobilized acetylcellulose membrane reactor, a mercury lamp, and a masterflex pump. As a result, under the various sterilization conditions examined, E. coli (102 cells per ml) was sterilized to <1% survival when the cell suspension flowed in this system at a mean residence time of 16.0 min under irradiation (1,800

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“…24 The applications of direct voltammetry of cell cultures in the detection of viable microbes in biological samples have been reported. [25][26][27] Research on the relationships between the population of living cells and the electrode behavior has been demonstrated. 28 There are many reports on electrochemical behavior of live cells.…”

Section: Introductionmentioning

confidence: 99%

Cyclic Voltammetric Measurements of Growth of Aspergillus terreus

et al. 2001

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A detailed study using the cyclic voltammogram was done on the live cells of Aspergillus terreus. The peak current values were obtained for different days of growth and plotted against time. The response of cyclic voltammogram showed the phases of the growth of the fungus. The growth curve obtained matched well with the conventional methodology, which assesses the increase of dry weight of the organisms against time. The electrochemical method is more advantageous because it is easy to assess and consumes less time. Further the electrochemical method clearly shows the decline phase which is generally not very defined in the conventional method of assessment of the growth curve. It was confirmed by further experiments that the metabolites were responsible for the anodic peak and not the biomass. Further work is in progress in order to analyze the metabolite(s) that is/are responsible for the anodic peak.

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Selective determination of microbial cells by graphite electrode modified with adsorbed 4,4′-bipyridine

Cited by 23 publications

References 6 publications

Photoelectrochemical sterilization of microbial cells by semiconductor powders

Photoelectrochemical sterilization of microbial cells by semiconductor powders

Continuous-sterilization system that uses photosemiconductor powders

Cyclic Voltammetric Measurements of Growth of Aspergillus terreus

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