Selective determination of homocysteine levels in human plasma using a silver nanoparticle-based colorimetric assay

Leesutthiphonchai, Wiphawee; Dungchai, Wijitar; Siangproh, Weena; Ngamrojnavanich, Nattaya; Chailapakul, Orawon

doi:10.1016/j.talanta.2011.04.041

Cited by 59 publications

(25 citation statements)

References 44 publications

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“…Additionally, AgNPs have shown some unique characteristics and advantages over AuNPs to a certain degree because they possess much higher absorption coefficients than AuNPs of the same size, [11,12] which can be as much as ,100fold greater. [13] More importantly, the cost of the preparation of AgNPs is much lower than that of AuNPs. Several AgNPs-based assays have been extensively developed and established for the detection of metal ions through the aggregation and isolation of the particles.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Assay for Al3+ Based on Alizarin Red S-functionalized Silver Nanoparticles

Liu

2014

Aust. J. Chem.

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Aluminium is absorbed by the intestines and is rapidly transported into bone, where it disrupts mineralization and bone cell growth and activity. Because aluminium is sequestered in bone for long periods, its toxic effects are cumulative. Even intermittent or low-dose use of aluminium-based materials (food, juice, drinking water containers) adds to the total load in bone. Development of fast and inexpensive sensors for aluminium, therefore, is still of great interest. We report here a simple, low-cost yet very sensitive and selective colorimetric assay for rapid (2-min) detection of Al 3þ in water with a 0.12-mM detection limit based on Alizarin Red S-functionalized silver nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Colorimetric Assay for Al3+ Based on Alizarin Red S-functionalized Silver Nanoparticles

Liu

2014

Aust. J. Chem.

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“…However, despite the natural interaction between ionic silver and thiols, studies on the thermodynamic data for the complex formation between Ag + and cysteine are not currently available. Regardless, AgNP had been used for the detection of cysteine in biological fluids due the high affinity that silver has for the -SH group in cysteine [56].…”

Section: Nature Of the Capping/protecting Agentmentioning

confidence: 99%

Biomedical Uses of Silver Nanoparticles: From Roman Wine Cups to Biomedical Devices

Weerasekera

Griffith

Alarcon

2015

Engineering Materials

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This chapter reviews the state of the art applications of silver nanoparticles in the biomedical sciences and health care, and aims to provide the reader with a general, but concise picture of the basic concepts that need to be considered when using silver nanoparticles (AgNP) in biological systems. We begin with a review of basic concepts on nanomaterials for biomedical applications. In the second part, we discuss a long-standing controversy regarding the risks versus benefits for the use of AgNP in the biomedical sciences. In the last section of this chapter, we present a discussion of future challenges regarding the use of AgNP in medicine.

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“…A suspension of silver nanoparticles (AgNPs), obtained from the Sensor Research Unit at Department of Chemistry, Chulalongkorn University, Thailand, was synthesized using chemical reduction 40–42 . Sodium borohydride (NaBH 4 ) and methylcellulose solutions were used as the reducing agent and stabilizer, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, nanoparticles have been widely used for colorimetric assays due to higher extinction coefficients than common organic dyes. Gold nanoparticle (AuNP) and silver nanoparticle (AgNP)-based colorimetric sensors have been reported for measuring thiols such as homocysteine (Hcy) 26–28 , glutathione (GSH) 26 , and cysteine (Cys) 29–31 in biological samples. The use of AgNPs has gained popularity for colorimetric sensing because AgNPs are inexpensive and have higher extinction coefficients than AuNPs 32–34 .…”

Section: Introductionmentioning

confidence: 99%

Determination of aerosol oxidative activity using silver nanoparticle aggregation on paper-based analytical devices

Dungchai

Sameenoi

Chailapakul

et al. 2013

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Airborne particulate matter (PM) pollution significantly impacts human health, but the cellular mechanisms of PM-induced toxicity remain poorly understood. A leading hypothesis on the effects of inhaled PM involves the generation of cellular oxidative stress. To investigate PM-induced oxidative stress, analytical methods have been developed to study the chemical oxidation of dithiothreitol (DTT) in the presence of PM. Although DTT readily reacts with several forms of reactive oxygen species, this molecule is not endogenously produced in biological systems. Glutathione (GSH), on the other hand, is an endogenous antioxidant that is produced throughout the body and is directly involved in combating oxidative stress in the lungs and other tissues. We report here a new method for measuring aerosol oxidative activity that uses silver nanoparticle (AgNP) aggregation coupled to glutathione (GSH) oxidation in a paper-based analytical device. In this assay, the residual reduced GSH from the oxidation of reduced GSH to its disulfide induces the aggregation of AgNPs on a paper-based analytical device, which produces a reddish-brown product. Two methods for aerosol oxidative reactivity are presented: one based on change in color intensity using a traditional paper-based techniques and one based on the length of the color product formed using a distance-based device. These methods were validated against traditional spectroscopic assays for DTT and GSH that employ Elman’s reagent. No significant difference was found between the levels measured by all three GSH methods (our two paper-based devices and the traditional method) at the 95% confidence level. PM reactivity towards GSH was less than towards DTT most likely due to the difference in the oxidation potential between the two molecules.

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Selective determination of homocysteine levels in human plasma using a silver nanoparticle-based colorimetric assay

Cited by 59 publications

References 44 publications

Colorimetric Assay for Al3+ Based on Alizarin Red S-functionalized Silver Nanoparticles

Colorimetric Assay for Al3+ Based on Alizarin Red S-functionalized Silver Nanoparticles

Biomedical Uses of Silver Nanoparticles: From Roman Wine Cups to Biomedical Devices

Determination of aerosol oxidative activity using silver nanoparticle aggregation on paper-based analytical devices

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