Selective detection of luminescence from semiconductor quantum dots by nanosecond time‐gated imaging with a colour‐masked CCD detector

Mitchell, Andrew C.; Dad, Shakeela; Morgan, Christopher G.

doi:10.1111/j.1365-2818.2008.01973.x

Cited by 7 publications

(4 citation statements)

References 10 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In practice this means that the smaller the QD, the bluer the light. In general, QDs have a relatively long lifetime, which provides the possibility to correct for background signals from short lived fluorescent species by time-gating techniques [ 33 , 34 ]. In addition, it was recently shown that the size of the QD also determines the lifetime, which increases with size [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Ishikawa-Ankerhold

Ankerhold

Drummen³

2012

Molecules

442

344

View full text Add to dashboard Cite

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Förster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research.

show abstract

Section: Introductionmentioning

confidence: 99%

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Ishikawa-Ankerhold

Ankerhold

Drummen³

2012

Molecules

442

344

View full text Add to dashboard Cite

show abstract

“…They can maintain high brightness even after repeated cycles of excitation and fluorescence for hours. Therefore, background signals from short-lived fluorescent species can be corrected by time-gating techniques, allowing QDs be used for long-term monitoring and cell-tracking studies [25, 26]. Second, each QD has a quite broad absorption spectrum and a narrow and symmetrical emission spectrum.…”

Section: Introductionmentioning

confidence: 99%

Application of fluorescence resonance energy transfer in protein studies

Yang

Zheng

2014

Journal of Molecular Structure

View full text Add to dashboard Cite

Since the physical process of fluorescence resonance energy transfer (FRET) was elucidated more than six decades ago, this peculiar fluorescence phenomenon has turned into a powerful tool for biomedical research due to its compatibility in scale with biological molecules as well as rapid developments in novel fluorophores and optical detection techniques. A wide variety of FRET approaches have been devised, each with its own advantages and drawbacks. Especially in the last decade or so, we are witnessing a flourish of FRET applications in biological investigations, many of which exemplify clever experimental design and rigorous analysis. Here we review the current stage of FRET methods development with the main focus on its applications in protein studies in biological systems, by summarizing the basic components of FRET techniques, most established quantification methods, as well as potential pitfalls, illustrated by example applications.

show abstract

“…Designing FLIM probes with longer lifetimes may prevent these effects [ 258 ]. Here, the usage of quantum dots with long fluorescence lifetimes may be an alternative [ 259 ]. Another challenge in using FLIM-FRET probes arises in experiments where the probes are overexpressed by cells.…”

Section: Challenges and Limitationsmentioning

confidence: 99%

Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring

Becker

Janssen

Layland

et al. 2021

Cancers

View full text Add to dashboard Cite

Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade, providing the potential to be translated into clinics for early-stage disease detection, in vitro drug screening, and drug efficacy studies in personalized medicine. Accessing tissue- and cell-specific spectral signatures, Raman microspectroscopy has emerged as a diagnostic tool to identify precancerous lesions, cancer stages, or cell malignancy. In vivo Raman measurements have been enabled by recent technological advances in Raman endoscopy and signal-enhancing setups such as coherent anti-stokes Raman spectroscopy or surface-enhanced Raman spectroscopy. FLIM enables in situ investigations of metabolic processes such as glycolysis, oxidative stress, or mitochondrial activity by using the autofluorescence of co-enzymes NADH and FAD, which are associated with intrinsic proteins as a direct measure of tumor metabolism, cell death stages and drug efficacy. The combination of non-invasive and molecular-sensitive in situ techniques and advanced 3D tumor models such as patient-derived organoids or microtumors allows the recapitulation of tumor physiology and metabolism in vitro and facilitates the screening for patient-individualized drug treatment options.

show abstract

Selective detection of luminescence from semiconductor quantum dots by nanosecond time‐gated imaging with a colour‐masked CCD detector

Cited by 7 publications

References 10 publications

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Application of fluorescence resonance energy transfer in protein studies

Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring

Contact Info

Product

Resources

About