Selective detection of antibodies in microstructured polymer optical fibers

Jensen, Jørgen Birger; Høiby, Poul E.; Emiliyanov, Grigoriy; Bang, Ole; Pedersen, Lars H.; Bjarklev, Anders

doi:10.1364/opex.13.005883

Cited by 235 publications

(143 citation statements)

References 1 publication

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…7,14 LPFGs can thus be used for biochemical sensing based on the evanescent-wave detection principle. [151][152][153][154][155] As shown in Fig. 21, a promising biochemical sensor based on a LPFG written in a PCF by a CO 2 laser has been demonstrated to detect the average thickness of a layer of biomolecules within a few nm.…”

Section: F Biochemical Sensorsmentioning

confidence: 99%

Review of long period fiber gratings written by CO2 laser

Wang

2010

Journal of Applied Physics

220

134

View full text Add to dashboard Cite

This paper presents a systematic review of long period fiber gratings (LPFGs) written by the CO2 laser irradiation technique. First, various fabrication techniques based on CO2 laser irradiations are demonstrated to write LPFGs in different types of optical fibers such as conventional glass fibers, solid-core photonic crystal fibers, and air-core photonic bandgap fibers. Second, possible mechanisms, e.g., residual stress relaxation, glass structure changes, and physical deformation, of refractive index modulations in the CO2-laser-induced LPFGs are analyzed. Third, asymmetrical mode coupling, resulting from single-side laser irradiation, is discussed to understand unique optical properties of the CO2-laser-induced LPFGs. Fourthly, several pretreament and post-treatment techniques are proposed to enhance the efficiency of grating fabrications. Fifthly, sensing applications of the CO2-laser-induced LPFGs are investigated to develop various LPFG-based temperature, strain, bend, torsion, pressure, and biochemical sensors. Finally, communication applications of the CO2-laser-induced LPFGs are investigated to develop various LPFG-based band-rejection filters, gain equalizers, polarizers, and couplers.

show abstract

Section: F Biochemical Sensorsmentioning

confidence: 99%

Review of long period fiber gratings written by CO2 laser

Wang

2010

Journal of Applied Physics

220

134

View full text Add to dashboard Cite

show abstract

“…Those advantages are due to the lower Young's modulus and higher thermo-optic coefficient of POFs [23,24]. In addition, polymers are clinically acceptable, which along with the flexible, non-brittle nature of the fibers makes these gratings an important candidate for in-vivo biosensing applications [25][26][27][28]. Despite of these promises not many commercial applications have been realized yet due to the high material loss of POFs.…”

Section: Introductionmentioning

confidence: 99%

Improved thermal and strain performance of annealed polymer optical fiber Bragg gratings

Yuan

Stefani

Bache

et al. 2011

Optics Communications

Self Cite

141

View full text Add to dashboard Cite

Abstract:We report on a detailed study of the inscription and characterization of fiber Bragg gratings (FBGs) in commercial step index polymer optical fibers (POFs). Through the growth dynamics of the gratings, we identify the effect of UV-induced heating during the grating inscription. We found that FBGs in annealed commercial POFs can offer more stable short-term performance at both higher temperature and larger strain. Furthermore, the FBGs' operational temperature and strain range without hysteresis was extended by the annealing process. We identified long-term stability problem of even the annealed POF FBGs. [7][8][9][10][11][12][13][14][15][16]. 325nm has been employed as a mainstream wavelength for writing grating in PMMA POFs [1][2][3][4][5][8][9][10][11][12][13][14][15]. Other wavelength such as 355nm obtained from a frequency-tripled Nd:YAG laser has been used to write grating in CYTOP fiber developed by Asahi Glass Co. and Keio University [15,16]. On the other hand, 800nm femtosecond pulses from Ti:Sapphire laser or its double frequency was mainly used for point by point direct writing [6] or grating writing with a phasemask [7]. However, the mechanism of index change does not appear to be fully understood [5,13,[18][19][20]. It is believed that more than one process is involved in the photo-induced refractive index changes and hence in the grating formation dynamics [18][19][20]. The widely accepted point is that the principle mechanism of index change is an increase due to the photo-induced polymerization of the unreacted monomers [5,[18][19][20], while laser-induced heating in the irradiated region during the inscription may also contribute to the index change [5]. Previous reports indicated that annealing of the POF before FBG inscription can relieve the frozen-in stress induced by the fiber drawing process [21] and increase the linear operation temperature range of FBGs [22]. However, the effect of annealing on the strain sensitivity performance was not yet considered. Polymer optical FBGs have shown great potential for sensor applications to sense for example temperature and strain with higher sensitivity and wider tunability than its silica counterpart [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Those advantages are due to the lower Young's modulus and higher thermo-optic coefficient of POFs [23,24]. In addition, polymers are clinically

show abstract

“…Vacuum was applied at the outlets of the two microwells to withdraw the liquid into the microwells. The solutions of BSA and DOTAP were allowed to adsorb to the PMMA microwell for 60 min and rinsed by 50 µl of PBS twice and dried with nitrogen [27][28][29][30]. Another flowcell was oxygen plasma treated in 5 min and closed with a COP slide before 50 µl of 50 mM DEGDME was 4!…”

Section: Deposition Of Blocking Agents Into Pmma Microfluidic Flow-cellsmentioning

confidence: 99%

“…The blocking of BSA is as good as that of dry DEGDME. BSA is widely used for blocking the non-specific adsorption of proteins [29], with typical concentrations ranging from 0.5 Ð 3 % w/w. Interestingly, the cationic DOTAP showed very good blocking with respect to protein non-specific adsorption.…”

Section: Blocking Effects Measured By Fluorescence Microscopymentioning

confidence: 99%

Evaluation of Different Nonspecific Binding Blocking Agents Deposited Inside Poly(methyl methacrylate) Microfluidic Flow-Cells

Gubala

Gandhiraman

et al. 2011

Langmuir

View full text Add to dashboard Cite

Poly(methyl methacrylate) (PMMA) flow-cells containing microwells were deposited with different nonspecific binding blocking agents namely bovine serum albumin (BSA), cationic lipid (DOTAP) and diethylene glycol dimethyl ether (DEGDME). Water contact angle (WCA) and atomic force microscope (AFM) measurements were carried out to confirm the successful depositions of BSA, DOTAP, DEGDME onto the PMMA surfaces. Fluorescent intensity measurements were performed to evaluate the degree of non-specific adsorption of Cy5-labeled anti-IgG proteins onto plain and oxygen plasma treated (PT) PMMA flow-cells as well as PMMA flow-cells deposited with different above-mentioned blocking agents. We then employed a label-free detection method called total internal reflection ellipsometry (TIRE) to evaluate the stability of the deposited blocking agents inside the PMMA flow-cells. It was found that while DOTAP was the best agent for blocking the non-specific adsorption, it could be removed from the PMMA surfaces of the flow-cells upon rinsing with phosphate buffer saline (PBS) and later deposited back onto the Au-coated glass sensing substrate of the TIRE. The removal of the blocking agents from PMMA surfaces and their deposition onto the sensing substrate were further manifested by measuring the kinetics and the amount of adsorbed anti-a-hCG proteins. Overall, the dry DEGDME coating by plasma enhanced chemical vapor deposition (PECVD) showed very good blocking and excellent stability for subsequent assay inside the microwells. Our results could be useful when one considers what blocking agents should be used for PMMA-based microfluidic immunosensor or biosensor devices by looking at both the blocking efficiency and the stability of the blocking agent.

show abstract

Selective detection of antibodies in microstructured polymer optical fibers

Cited by 235 publications

References 1 publication

Review of long period fiber gratings written by CO2 laser

Review of long period fiber gratings written by CO2 laser

Improved thermal and strain performance of annealed polymer optical fiber Bragg gratings

Evaluation of Different Nonspecific Binding Blocking Agents Deposited Inside Poly(methyl methacrylate) Microfluidic Flow-Cells

Contact Info

Product

Resources

About