Selective detection, enumeration and identification of potentially probiotic Lactobacillus and Bifidobacterium species in mixed bacterial populations

Charteris, William P; Kelly, Philip M.; Morelli, Lorenzo; Collins, John

doi:10.1016/s0168-1605(96)01222-6

Cited by 169 publications

(131 citation statements)

References 211 publications

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“…The residual viable counts for all the isolates were greater than 10 6 cfu/ml after 3 hours at pH 3. The observed results in this study, correlated with reports from Charteris et al (1997).…”

Section: Acid Tolerancesupporting

confidence: 93%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

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Section: Acid Tolerancesupporting

confidence: 93%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

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“…Thus the standardisation of a method for the selective or differential count of Bifidobacterium sp in the presence of yoghurt cultures has become very important. Various methods for the selective count of Bifidobacterium sp in yoghurt, based on pure cultures, have been proposed (1)(2)(3)(6)(7)(8)(10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of culture media for counts of Bifidobacterium animalis in the presence of yoghurt bacteria

Moriya¹,

Fachin²,

Gândara³

et al. 2006

Braz. J. Microbiol.

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Much attention has been paid to the use of Bifidobacterium sp. in yoghurts due to their excellent therapeutic and nutritional properties. However, in order to present health properties, it is recommended that each commercial product should indicate the minimum daily amount required for it to confer health benefit(s) based on in vitro and human studies. Besides the inherent low growth of Bifidobacterium sp in milk, there is a need for a reliable method for counting Bifidobacterium sp in the presence of yoghurt bacteria. This study evaluated the use of the media M-MRS, MRS-NNLP and RCPB pH5 aimed at counting the number of Bifidobacterium animalis subesp. lactis in the presence of Streptococcus thermophilus and Lactobacillus delbrueckii subsp bulgaricus after yoghurt fermentation. The M-MRS medium was not selective, allowing for the growth of L. delbrueckii subsp bulgaricus. MRS-NNLP medium presented a good selectivity for B. animalis Bb12 with a slight reduction in the cell count of this microrganism when compared it to the standard MRS medium in pure culture. MRS-NNLP medium was considered a good option to enumerate B. animalis Bb12 although the reduction found in pure culture due to the low difference between the counts. The medium RCPB pH5 presented differentiated growth of B. animalis Bb12 in relation to the yoghurt bacteria and a cell recovery equal to that of the standard MRS, being considered the best option to enumerate Bifidobacterium sp in the presence of yoghurt bacteria.

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“…Antimicrobial effects of lactic acid bacteria are formed by producing some substances such as organic acids (lactic, acetic, propionic acids), carbon dioxide, hydrogen peroxide, diacetyl, low molecular weight antimicrobial substances and bacteriocins [14,15]. Till today there are some researches on showing that different species produce different antimicrobial substances.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Probiotic Properties of Lactic Acid Bacteria from Human Milk

Amenu¹

2015

J Med Microb Diagn

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Probiotics are preparation of live microorganisms that have beneficial effects on the health of their host. Although probiotic strains can be isolated from many sources, for human applications, the main criteria are that it must be from human origin. Milk is an important nutrient source for neonates. Previous studies have shown that human breast milk has beneficial effects on the health of neonates. One reason for these beneficial effects might be explained by the micro flora of human breast milk including beneficial lactic acid-producing bacteria. The main aim of this paper is to review best practices in isolation and characterization of lactic acid bacteria from human milk by biochemical and molecular methods and also determine the probiotic properties of lactic acid-producing bacteria. Most lactic acidproducing bacteria have effective antimicrobial activity against food-borne pathogens like Salmonella, thyphimurium CCM 5445, Escherichia coli O157:H7 NCTC 129000 and Escherichia coli NRRL B-3008. Data show that these isolates possess probiotic properties, as demonstrated by using amplification and restriction fragment length polymorphism (RFLP) of 16S ribosomal DNA (rDNA) and 16S sequencing. Therefore, it is observed that, human milk is a source of potential probiotic strains.

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Selective detection, enumeration and identification of potentially probiotic Lactobacillus and Bifidobacterium species in mixed bacterial populations

Cited by 169 publications

References 211 publications

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Evaluation of culture media for counts of Bifidobacterium animalis in the presence of yoghurt bacteria

Probiotic Properties of Lactic Acid Bacteria from Human Milk

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