Selective Desulfurization of Dibenzothiophene by
            <i>Rhodococcus erythropolis</i>
            D-1

Izumi, Yoshikazu; Ohshiro, Takashi; Ogino, Hiroshi; Hine, Yoshimitsu; Shimao, Masayuki

doi:10.1128/aem.60.1.223-226.1994

Cited by 235 publications

(87 citation statements)

References 12 publications

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“…GC and HPLC were performed as previously described [11]. The protein concentration was determined by the method of Bradford.…”

Section: Methodsmentioning

confidence: 99%

“…The medium for the cultivation, medium A, was as described previously [11]. Rhodococcus erythropolis D-1 was grown in 500 ml of medium A containing DBT at 0.136 mM in a 2-1 shaking flask.…”

Section: Cultural Conditionsmentioning

confidence: 99%

“…Figure 1 However, enzymatic conversion of DBT to 2-HBP has never been reported. In a previous study, we isolated R. erythropolis D-I which utilizes DBT as a sole source of sulfur and demonstrated the efficient desulfurization of DBT to 2-HBP by both growing and resting cells of this strain [11].…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1

Ohshiro

1994

FEMS Microbiology Letters

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The enzymatic desulfurization of dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP) was detected in extracts of Rhodococcus erythropolis D-1 grown on DBT as a sole source of sulfur. In the dialyzed cell-free extracts, NADH was absolutely required for the activity. DBT desulfurization proceeded linearly with the reaction time, and stoichiometric amounts of 2-HBP were finally formed. The activity was stimulated by increasing the protein and NADH concentrations up to 5 mg ml-1 and 5 mM, respectively, when 0.1 mM DBT was present in the reaction mixture.

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“…GC and HPLC were performed as previously described [11]. The protein concentration was determined by the method of Bradford.…”

Section: Methodsmentioning

confidence: 99%

“…The medium for the cultivation, medium A, was as described previously [11]. Rhodococcus erythropolis D-1 was grown in 500 ml of medium A containing DBT at 0.136 mM in a 2-1 shaking flask.…”

Section: Cultural Conditionsmentioning

confidence: 99%

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Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1

Ohshiro

1994

FEMS Microbiology Letters

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“…The combustion of fossil fuels releases vast amounts of sulphur oxides into the atmosphere and causes serious environmental problems as acid rain (Olmo et al, 2005). To decrease sulphur oxide emissions into the environment, the reduction of the sulphur content in fossil fuels is usually carried out by hydrodesulphurization, which uses high temperature and pressure (Izumi et al, 1994). Various types of sulphur compounds can be removed by hydrodesulphurization, but a number of heterocyclic sulphur compounds are not (Soleimani et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the dibenzothiophene metabolic pathway in a newly isolatedRhodococcusspp.

Akhtar¹,

Ghauri²,

Anwar³

et al. 2009

FEMS Microbiology Letters

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Out of 17 samples collected from diverse environments, 110 bacterial isolates of varied characteristics were screened for their dibenzothiophene-desulphurizing activity. A single isolate, Eu-32, originating from a soil sample taken from the roots of a eucalyptus tree, displayed dibenzothiophene-desulphurizing activity. This isolate metabolized dibenzothiophene to 2-hydroxybiphenyl (2-HBP), as detected by HPLC, and was also able to use other organic sulphur compounds as a sole sulphur source. Based on morphological, biochemical and molecular studies, it was found that the organism belongs to the genus Rhodococcus, with a maximum of 95% identity to species in this genus for the partial sequence of the 16S rRNA gene. Isolate Eu-32 could desulphurize 0.2 mM dibenzothiophene to 2-HBP in 72 h at a temperature of 30 degrees C and pH 7.0. The structure and molecular mass of metabolites produced from dibenzothiophene desulphurization were identified by GC-MS, and two sulphur-free products, 2-HBP and biphenyl, were detected in ethyl acetate extract. It was concluded that isolate Eu-32 is a unique desulphurizing biocatalyst that desulphurizes dibenzothiophene through an extended, sulphur-specific degradation pathway with the selective cleavage of C-S bonds.

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“…Several bacterial genera such as Bacillus [10], Pseudomonas [10][11][12][13][14][15][16], Serratia [10], Escherichia [10], Desulfovibrio [17,18], Rhodococcus [19], Rhizobium [20], Beijerinckia [21,22], Ralstonia [23,24], Corynebacterium [25], Staphylococcus [26], Xanthomonas [27], Terrabacter [28], and Brevibacterium [29] contain species able to degrade carbazole, DBT, or DBF. These bacteria have been isolated from activated sludge, tar-contaminated soil, compost soil, and river sedi-ments.…”

Section: Introductionmentioning

confidence: 99%

Degradation of Carbazole, Dibenzothiophene, and Dibenzofuran at Low Temperature by Pseudomonas Sp. Strain C3211

Jensen¹,

Finster²,

Karlson³

2003

Environ Toxicol Chem

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Pseudomonas sp. strain C3211 was isolated from a temperate climate soil contaminated with creosote. This strain was able to degrade carbazole, dibenzothiophene and dibenzofuran at 10 degrees C with acetone as a co-substrate. When dibenzothiophene was degraded by strain C3211, an orange compound, which absorbed at 472 nm, accumulated in the medium. Degradation of dibenzofuran was followed by accumulation of a yellowish compound, absorbing at 462 nm. The temperature optimum of strain C3211 for degradation of dibenzothiophene and dibenzofuran was at 20 to 21 degrees C, while the maximum temperature for degradation was at 27 degrees C. Both compounds were degraded at 4 degrees C. Degradation at 10 degrees C was faster than degradation at 25 degrees C. This indicates that strain C3211 is adapted to life at low temperatures.

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Selective Desulfurization of Dibenzothiophene by Rhodococcus erythropolis D-1

Cited by 235 publications

References 12 publications

Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1

Enzymatic desulfurization of dibenzothiophene by a cell-free system of Rhodococcus erythropolis D-1

Analysis of the dibenzothiophene metabolic pathway in a newly isolatedRhodococcusspp.

Degradation of Carbazole, Dibenzothiophene, and Dibenzofuran at Low Temperature by Pseudomonas Sp. Strain C3211

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