Selective decreases in levels of mRNA encoding a water channel (AQP3) in ileal mucosa after ileostomy in the rat

Purdy, M. J.; Cima, Robert R.; Doble, Marc A.; Klein, Michael A.; Zinner, Michael J.; Soybel, David I.

doi:10.1016/s1091-255x(99)80009-2

Cited by 13 publications

(6 citation statements)

References 16 publications

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“…On the other hand, no significant changes of AQP1 and AQP4 mRNA expression were observed in the GI tract in either experimental model (data not shown). Moreover, AQP3 has been suggested to function in the small and the large intestine by previous studies [22,23]. However, significant change of AQP3 mRNA expression was not demonstrated in the present models.…”

Section: Discussioncontrasting

confidence: 67%

Aquaporin 8 mRNA expression after intestinal resection in rat

Koyama¹,

Kameyama²,

Sakata³

et al. 2014

OJGas

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Backgrounds: Aquaporins (AQPs), the mammalian water channels, have been localized in various organs, including the gastrointestinal (GI) tract. We examined AQPs expression in rat models of massive intestineal resection to determine the functions of AQPs in the GI tract. Methods: Female Sprague-Dawley rats (n = 15) underwent 90% resection of the small intestine, and Female Wistar-Kyoto rats (n = 10), received subtotal colectomy, and were sacrificed following the operations. RNase protection assay and quantitative reverse transcription-polymerase chain reaction (RT-PCR) were performed to measure the AQPs mRNA expression in the GI tract. Immunohistochemistry was performed to confirm AQP8 protein expression. Results: AQP8 mRNA expression (mean ± standard error), was enhanced in the jejunum of the short bowel rats at days 7 and 14 (37.6% ± 1.4% and 18.5% ± 2.4%, respectively, p < 0.01). Enhancement of AQP8 mRNA was also observed in the remnant rectum of the subtotal colectomized rats at both days 21 and 42 (116.1% ± 4.5% and 143.3% ± 7.4%, respectively, p < 0.01). Immunohistochemistry demonstrated enhanced AQP8 expression in the remnant rectum of the subtotal colectomized rats. No intensive change was observed with other AQPs in both models. Conclusions: Our results suggest a compensatory role of AQP8 in the maintenance of intestinal fluid balance.

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Section: Discussioncontrasting

confidence: 67%

Aquaporin 8 mRNA expression after intestinal resection in rat

Koyama¹,

Kameyama²,

Sakata³

et al. 2014

OJGas

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“…Fasting results in an impairment in urinary concentrating ability in rats because of a downregulation of AQP2 mRNA and protein in the kidneys (Amlal et al 2001), whereas water deprivation has been shown to increase AQP2 message and protein levels in the distal colon (Gallardo et al 2001). Surgical ileostomy in rats decreases AQP3 mRNA expression in the distal ileum, whereas sham surgery results in an increase in AQP3 mRNA expression (Purdy et al 1999). Finally, age-related polyuria has been associated with a decrease in AQP2 and AQP3 expression in the medullary collecting duct (Preisser et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn’s disease and infectious colitis

et al. 2004

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Colitis is associated with alterations in electrolyte and water transport. These changes give rise to some of the symptoms experienced by patients with colitis. Alterations in fluid flux may also contribute to increased susceptibility to mucosal injury. Recently, endogenous water channel proteins (aquaporins; AQPs), have been identified in colonic tissue. The expression of AQP4, AQP7 and AQP8 was examined, via reverse transcription/polymerase chain reaction, Western blotting and immunohistochemistry, in a murine model of colitis and in patients with inflammatory bowel disease or infectious colitis. Colitis was induced in C57BL/6 mice by the addition of 2.5% dextran sodium sulphate (DSS) to their drinking water. AQP expression in these mice was assessed following 12 h to 7 days of DSS exposure and during the recovery phase from 1 to 15 days following cessation of DSS exposure. Colonic water transport was measured after 1 and 3 days of DSS and following 7 days of recovery. The expression of AQP4 and AQP8 mRNA was significantly decreased after 12-24 h of DSS exposure and remained depressed throughout the treatment period. Expression of AQP7 was more variable. Protein expression followed a similar pattern to that observed for AQP mRNA. Significant alteration in colonic fluid secretion was correlated with reduced expression of AQP isoforms. Significantly, patients with active ulcerative colonic, Crohn's colitis or infectious colitis had similar dramatic reductions in AQP expression that appeared to be correlated with disease activity. Thus, colonic injury in both mouse and man is associated with a downregulation in AQP expression.

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“…Besides, AQP1, first known as CHIP, has been shown to be abundantly expressed in the rat duodenal and ideal lacteals [ 72 ]. Notably, the AQP3 mRNA level is increased along the length of the intestine in rats, with abundant expression in the distal ileum [ 73 ]. Using immunohistochemistry, AQP3 staining is confined to the basolateral membrane of absorptive epithelial cells lining the lumen and to the neck of crypts [ 32 ].…”

Section: Expression Distribution and Role Of Aqps In The Intestinmentioning

confidence: 99%

Expression, Distribution and Role of Aquaporin Water Channels in Human and Animal Stomach and Intestines

Zhu

Chen

Jiang

2016

IJMS

127

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Stomach and intestines are involved in the secretion of gastrointestinal fluids and the absorption of nutrients and fluids, which ensure normal gut functions. Aquaporin water channels (AQPs) represent a major transcellular route for water transport in the gastrointestinal tract. Until now, at least 11 AQPs (AQP1–11) have been found to be present in the stomach, small and large intestines. These AQPs are distributed in different cell types in the stomach and intestines, including gastric epithelial cells, gastric glands cells, absorptive epithelial cells (enterocytes), goblet cells and Paneth cells. AQP1 is abundantly distributed in the endothelial cells of the gastrointestinal tract. AQP3 and AQP4 are mainly distributed in the basolateral membrane of epithelial cells in the stomach and intestines. AQP7, AQP8, AQP10 and AQP11 are distributed in the apical of enterocytes in the small and large intestines. Although AQP-null mice displayed almost no phenotypes in gastrointestinal tracts, the alterations of the expression and localization of these AQPs have been shown to be associated with the pathology of gastrointestinal disorders, which suggests that AQPs play important roles serving as potential therapeutic targets. Therefore, this review provides an overview of the expression, localization and distribution of AQPs in the stomach, small and large intestine of human and animals. Furthermore, this review emphasizes the potential roles of AQPs in the physiology and pathophysiology of stomach and intestines.

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Selective decreases in levels of mRNA encoding a water channel (AQP3) in ileal mucosa after ileostomy in the rat

Cited by 13 publications

References 16 publications

Aquaporin 8 mRNA expression after intestinal resection in rat

Aquaporin 8 mRNA expression after intestinal resection in rat

Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn’s disease and infectious colitis

Expression, Distribution and Role of Aquaporin Water Channels in Human and Animal Stomach and Intestines

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