Selective cytotoxicity of peritoneal leucocytes for neoplastic cells

Modulation of Cell Proliferation by Macrophages: A Possible Function Apart from Cytotoxic Tumour Rejection

“…So far, conclusions on the outcome of such interactions have been based solely on the morphological consequences, i.e. either a decrease in number and changes in shape of targets as observed in the cultures (Hibbs et at., 1972;Hibbs, 1973) or enumeration of targets remaining detectable after interaction (Evans and Alexander, 1972;Alexander and Evans, 1971;Holtermann et al, 1973). Using these parameters, cytocidal, cytolytic or cytotoxic effects accompanied by a marked diminishment in tumour target numbers were accurately reflected without in any way disclosing, however, of the less obvious cytostatic effects.…”

“…Moreover, in cultures of sensitized macrophages and specific antigens, antigenically unrelated tumour cells can be damaged as "innocent bystanders " (Evans and Alexander, 1972). Finally, normal macrophages activated in vivo and in vitro by any of a large array of nonspecific stimulants of natural immiunity acquire the capacity effectively to inhibit tumour growth in vitro (Alexander and Evans, 1971;Keller and Jones, 1971;Keller, 1973a, b; Hibbs, Lambert and Remington, 1972;Holtermann, Klein and Casale, 1973) and in vivo (Keller and Hess, 1972).…”

Natural cytotoxicity of haemopoietic cell populations against murine lymphoid tumours

“…Cell-mediated cytotoxicity, as assayed by the lysis of tumour cell lines, has been reported in a number of different systems using human blood leucocytes (Takasugi, Mickey and Terasaki, 1973; Petranyi et al, 1974), mouse spleen cells Greenberg and Playfair, 1974) and rat spleen cells Holterman, Klein and Casale, 1973) (Kiessling et al, 1975a) an increased incidence in nude mice (Kiessling et al, 1975b) and a lability in tissue culture at 37°C (Herberman et al, 1975b). Recent studies have identified a specific cell-surface alloantigen on the NK cell (Glimcher, Shen and Cantor, 1977) and may provide an approach to its eventual isolation and precise characterization.…”

“…Cell-mediated cytotoxicity, as assayed by the lysis of tumour cell lines, has been reported in a number of different systems using human blood leucocytes (Takasugi, Mickey and Terasaki, 1973;Petranyi et al, 1974), mouse spleen cells Greenberg and Playfair, 1974) and rat spleen cells Holterman, Klein and Casale, 1973) from normal donors. In 1975 reports from several laboratories clearly demonstrated the killing of various mouse lymphoma lines by spleen-cell suspensions from non-immune animals.…”

Abrogation of Antitumor Effects of Corynebacterium parvum and BeG by Antimacrophage Agents: Brief Communication 2

“…Depending on the route and timing of administration of such agents, macrophages acquire the capacity to kill neoplastic cells in vitro with considerable selectivity (8,(10)(11)(12)(13). Other observations imply an inverse relationship between the macrophage content of tumors and tumor regression (14) or metastasis (15).…”