Selective Cytotoxicity of Manganese Nanoparticles against Human Glioblastoma Cells

Разумов, И. А.; Zavyalov, E. L.; Troitskii, S. Yu.; Romashchenko, A. V.; Petrovskii, D. V.; Купер, К. Э.; Мошкин, М. П.

doi:10.1007/s10517-017-3849-0

Cited by 16 publications

(13 citation statements)

References 16 publications

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“…5Mn‐MBGNs exhibited the most pronounced dose‐dependent cytotoxic effect on BMSCs with a significant increase of cell viability in the lower concentration group but a significant decrease when applied in higher concentrations. It has previously been reported that Mn shows concentration‐dependent toxic properties with pronounced systemic neurotoxicity 43‐46 . Furthermore, a very similar concentration‐dependent cytotoxic effect for 5Mn‐MBGNs has been shown before in a study conducted by our group 7 .…”

Section: Discussionsupporting

confidence: 84%

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Westhauser

Wilkesmann

Nawaz

et al. 2020

J Biomedical Materials Res

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Mesoporous bioactive glass nanoparticles (MBGNs) have demonstrated promising properties for the local delivery of therapeutically active ions with the aim to improve their osteogenic properties. Manganese (Mn), zinc (Zn), and copper (Cu) ions have already shown promising pro‐osteogenic properties. Therefore, the concentration‐dependent impact of MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs containing 5 mol% of either Mn, Zn, or Cu (composition in mol%: 70 SiO2, 25 CaO, 5 MnO/ZnO/CuO) on the viability and osteogenic differentiation of human marrow‐derived mesenchymal stromal cells (BMSCs) was assessed in this study. Mn‐doped MBGNs (5Mn‐MBGNs) showed a small “therapeutic window” with a dose‐dependent negative impact on cell viability but increasing pro‐osteogenic features alongside increasing Mn concentrations. Due to a constant release of Zn, 5Zn‐MBGNs showed good cytocompatibility and upregulated the expression of genes encoding for relevant members of the osseous extracellular matrix during the later stages of cultivation. In contrast to all other groups, BMSC viability increased with increasing concentration of Cu‐doped MBGNs (5Cu‐MBGNs). Furthermore, 5Cu‐MBGNs induced an increase in alkaline phosphatase activity. In conclusion, doping with Mn, Zn, or Cu can enhance the biological properties of MBGNs in different ways for their potential use in bone regeneration approaches.

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Section: Discussionsupporting

confidence: 84%

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Westhauser

Wilkesmann

Nawaz

et al. 2020

J Biomedical Materials Res

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“…Manganese(III) oxide nanofibers proved capable of label-free zeptomolar detection of DNA hybridization, with potential applications in in situ detection of cancerous mutations . Also, manganese nanoparticles were shown to be selectively toxic to glioblastoma cells compared to human embryo fibroblasts . Manganese dioxide nanoparticles can also be effective antibacterial agents thanks to their ability to penetrate the bacterial cell wall and induce cytoplasmic leakage …”

Section: Introductionmentioning

confidence: 99%

“…24 Also, manganese nanoparticles were shown to be selectively toxic to glioblastoma cells compared to human embryo fibroblasts. 25 Manganese dioxide nanoparticles can also be effective antibacterial agents thanks to their ability to penetrate the bacterial cell wall and induce cytoplasmic leakage. 26 However, in the domain of bone engineering, manganese has not been studied as abundantly as expected.…”

Section: Introductionmentioning

confidence: 99%

Sic Parvis Magna: Manganese-Substituted Tricalcium Phosphate and Its Biophysical Properties

Rau

Фадеева

Фомин

et al. 2019

ACS Biomater. Sci. Eng.

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Succeeding in the substitution of pharmaceutical compounds with ions deliverable with the use of resorbable biomaterials could have far-reaching benefits for medicine and economy. Calcium phosphates are known as excellent accommodators of foreign ions. Manganese, the fifth most abundant metal on Earth was studied here as an ionic dopant in β-tricalcium phosphate (β-TCP) ceramics. β-TCP containing different amounts of Mn2+ ions per Mn x Ca3–x (PO4)2 formula (x = 0, 0.001, 0.01, and 0.1) was investigated for a range of physicochemical and biological properties. The results suggested the role of Mn2+ as a structure booster, not breaker. Mn2+ ions increased the size of coherent X-ray scattering regions averaged across all crystallographic directions and also lowered the temperature of transformation of the hydroxyapatite precursor to β-TCP. The particle size increased fivefold, from 20 to 100 nm, in the 650–750 °C region, indicating that the reaction of formation of β-TCP was accompanied by a considerable degree of grain growth. The splitting of the antisymmetric stretching mode of the phosphate tetrahedron occurred proportionally to the Mn2+ content in the material, while electron paramagnetic resonance spectra suggested that Mn2+ might substitute for three out of five possible calcium ion positions in the unit cell of β-TCP. The biological effects of Mn-free β-TCP and Mn-doped β-TCP were selective: moderately proliferative to mammalian cells, moderately inhibitory to bacteria, and insignificant to fungi. Unlike pure β-TCP, β-TCP doped with the highest concentration of Mn2+ ions significantly inhibited the growth of all bacterial species tested: Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis. The overall effect against the Gram-positive bacteria was more intense than against the Gram-negative microorganisms. Meanwhile, β-TCP alone had an augmentative effect of the viability of adipose-derived mesenchymal stem cells (ADMSCs) and the addition of Mn2+ tended to reduce the extent of this augmentative effect, but without imparting any toxicity. For all Mn-doped β-TCP concentrations except the highest, the cell viability after 72 h incubation was significantly higher than that of the negative control. Assays evaluating the effect of Mn2+-containing β-TCP formulations on the differentiation of ADMSCs into three different lineagesosteogenic, adipogenic, and chondrogenicdemonstrated no inhibitory or adverse effects compared to pure β-TCP and powder-free positive controls. Still, β-TCP delivering the lowest amount of Mn2+ seemed most effective in sustaining the differentiation process toward all three phenotypes, indicating that the dose of Mn2+ in β-TCP need not be excessive to be effective.

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“…For the first time, we present a systematic characterization of manganese oxide nanocrystals from In-house vs commercialized sources (US Research Nanomaterials (US Nano), and Nanoshel) pre- and post-encapsulation in PLGA to evaluate their use as T 1 MRI contrast agents. Often, researchers will use these purchased nanomaterials in “as is” condition without verifying the products’ advertised properties, − which if inaccurate, could unexpectedly affect experimental results. Thus, we sought to compare different manganese oxide NPs (MnO, Mn 2 O 3 , and Mn 3 O 4 ) from In-house, US Nano, and Nanoshel for size, morphology, chemistry, loading, controlled release of manganese ions, and MRI signal.…”

Section: Introductionmentioning

confidence: 99%

Caveat Emptor: Commercialized Manganese Oxide Nanoparticles Exhibit Unintended Properties

Martinez de la Torre,

Freshwater,

Looney-Sanders

et al. 2023

ACS Omega

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Nano-encapsulated manganese oxide (NEMO) particles are noteworthy contrast agents for magnetic resonance imaging (MRI) due to their bright, pH-switchable signal (“OFF” to “ON” at low pH), high metal loading, and targeting capability for increased specificity. For the first time, we performed a head-to-head comparison of NEMO particles from In-house and commercialized sources (US Nano vs Nanoshel) to assess their potential as bright T1 MRI contrast agents. Manganese oxide nanocrystals (MnO, Mn2O3, and Mn3O4) were systematically evaluated for size, chemistry, release of manganese ions, and MRI signal pre- and post-encapsulation within poly(lactic-co-glycolic acid) (PLGA). Suprisingly, a majority of the commercialized formulations were not as advertised by displaying unintended sizes, morphologies, chemistry, dissolution profiles, and/or MRI signal that precludes in vivo use. US Nano’s Mn3O4 and Mn2O3 nanocrystals contained impurities that impacted Mn ion release as well as micron-sized rodlike structures. Nanoshel’s MnO and Mn2O3 nanoparticles had very large hydrodynamic sizes (>600 nm). In-house MnO and Nanoshel’s Mn3O4 nanoparticles demonstrated the best characteristics with brighter T1 MRI signals, small hydrodynamic sizes, and high encapsulation efficiencies. Our findings highlight that researchers must confirm the properties of purchased nanomaterials before utilizing them in desired applications, as their experimental success may be impacted.

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Selective Cytotoxicity of Manganese Nanoparticles against Human Glioblastoma Cells

Cited by 16 publications

References 16 publications

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles

Sic Parvis Magna: Manganese-Substituted Tricalcium Phosphate and Its Biophysical Properties

Caveat Emptor: Commercialized Manganese Oxide Nanoparticles Exhibit Unintended Properties

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