Selective cytotoxicity of arene tricarbonylchromium towards tumour cell lines

Elloumi-Mseddi, Jihene; Mnif, Sami; Akacha, Nejmeddine; Hakim, Bochra; Pigeon, Pascal; Jaouen, Gérard; Top, Siden; Aifa, Sami

doi:10.1016/j.jorganchem.2018.01.036

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…The comparison of our results with those found in the literature is delicate because the cell types and exposure conditions were different. However, the IC 50 of Cr in HepaRG cells was close to those obtained for other human cell models. , For Co, there is a wide variation in IC 50 among published studies. , The IC 50 of Co in HepaRG cells was higher than that in keratinocytes for similar exposure conditions. For Ni, the only cytotoxicity study on human hepatocytes found in the literature presented an IC 50 slightly lower than ours for a 24 h exposure .…”

Section: Discussionsupporting

confidence: 79%

“…However, the IC 50 of Cr in HepaRG cells was close to those obtained for other human cell models. 43,45 For Co, there is a wide variation in IC 50 among published studies. 58,59 The IC 50 of Co in HepaRG cells was higher than that in keratinocytes 42 for similar exposure conditions.…”

Section: ■ Discussionmentioning

confidence: 99%

“…The half-maximal inhibitory concentration (IC 50 ) represents the concentration that inhibits 50% of cellular biological functions. After a review of the literature, − the cells were incubated with 10 levels of Co, Cr, and Ni concentrations for 48 h at 37 °C in a FBS-free medium: 10, 50, 100, 250, 500, 1000, 2500, 5000, 10 000, and 50 000 μM for Co and Ni and 0.5, 5, 10, 50, 100, 500, 1000, 5000, 10 000, and 50 000 μM for Cr.…”

Section: Methodsmentioning

confidence: 99%

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Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Bellouard

Gasser

Lenglet

et al. 2022

Chem. Res. Toxicol.

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Cobalt, chromium, and nickel are used in orthopedic prostheses. They can be released, accumulate in many organs, and be toxic. The aim of this study is to evaluate the cytotoxicity of these metals on human hepatocytes and to improve our knowledge of their cellular toxicity mechanisms by metabolomic analysis. HepaRG cells were incubated for 48 h with increasing concentrations of metals to determine their IC50. Then, a nontargeted metabolomic study using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) was done at IC50 and at a lower concentration (100 nM), near to those found in the blood and liver of patients with prostheses. IC50 were defined at 940, 2, and 1380 μM for Co, Cr, and Ni, respectively. In vitro, Cr appears to be much more toxic than Co and Ni. Metabolomic analysis revealed the disruption of metabolic pathways from the low concentration of 100 nM, in particular tryptophan metabolism and lipid metabolism illustrated by an increase in phenylacetylglycine, a marker of phospholipidosis, for all three metals. They also appear to be responsible for oxidative stress. Dysregulation of these pathways impacts hepatocyte metabolism and may result in hepatotoxicity. Further investigations on accessible biological matrices should be conducted to correlate our in vitro results with the clinical data of prostheses-bearing patients.

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Section: Discussionsupporting

confidence: 79%

Section: ■ Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Toxicity and Metabolomic Impact of Cobalt, Chromium, and Nickel Exposure on HepaRG Hepatocytes

Bellouard

Gasser

Lenglet

et al. 2022

Chem. Res. Toxicol.

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“…These finding could use the incorporation of CdCl 2 in organic compounds in order to create a tumour-specific toxicity. In fact a selective cytoxicity against cancer cell lines was obtained with chromium present in some arene tricarbonylchromium compounds vs the high toxicity of chromium in K 2 Cr 2 O 7 [55].…”

Section: Toxicity Testmentioning

confidence: 99%

Untitled

2021

Journal of Materials Sciences and Applications

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The present study, deals with the investigation of new synthesized Urea-CdCl 2 complex [(NH 2 CONH 2 )CdCl 2 ] properties at room temperature. X-ray diffraction (XRD), infrared (IR), UV visible (UV-vis), photoluminescence (PL) measurements are carried out to characterize structural and optical properties. The toxicity of this complex was tested as well.The obtained results confirmed the formula (NH 2 CONH 2 )CdCl 2 for the formed complex, in full agreement with the literature data.XRD indicates that this compound crystallizes at room temperature in the triclinic system with space group. The average crystallite size estimated from Debye Scherrer model was found to be about 61 nm. IR spectrum confirmed the presence of the organic chains of the Urea-CdCl 2 complex. Furthermore, it proved that the cohesion between the organic and inorganic groups is insured by the Cd-O bonds.Besides, the optical properties were examined by UV-vis and PL spectrocopies. The UV-vis absorption measurements showed that the optical absorbance is higher in the Ultraviolet (λ < 300 nm) domain. The optical band gap (E g ) is deduced to be 4.02 eV. Optical parameters were estimated from the absorption data. Moreover, the toxicity of the Urea-CdCl 2 Complex was tested against normal kidney cell line (HEK293) and two cancers cell lines (MDA and T47D). The obtained results showed that Urea-CdCl 2 improved the toxicity against the breast cancer line T47D.

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