Selective Culture of Mesodermal Progenitor Cells

Trombi, Luisa; Pacini, Stefania; Montali, Marina; Fazzi, Rita; Chiellini, Emo; Ikehara, Susumu; Petrini, Mario

doi:10.1089/scd.2009.0054

Cited by 27 publications

(58 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The selective culture conditions described here have allowed the isolation of a novel adherent and almost monomorphic cell population as 1.0% of the hBM-MNCs (0.5 -2.0 x 10 6 hBM-MNCs from 5 -10 ml of fresh BM samples) 5,6 . We identified these large (40 -60 µm in diameter), rounded, quiescent, Ki-67-negative cells as MPCs 5 .…”

Section: Representative Resultsmentioning

confidence: 99%

“…In our hands replacement of FBS with PhABS in primary cultures from hBM-MNCs, combined with high density seeding on hydrophobic plastics, led to the isolation of a novel highly adherent cell population with distinct biological features named MPCs 5,6 . We observed that the addition of small percentages of PhABS to FBS primary cultures also allowed MPC isolation, suggesting the presence of MPC inducing agents in the human serum 6 . At the moment, the MPC isolation/ characterization protocol is a unique method available to obtain almost pure MPCs.…”

Section: Representative Resultsmentioning

confidence: 99%

“…Nonetheless, the initial enthusiasm about mesodermal differentiation potential of MPCs is actually waning. In fact, after more than 7 years of studies on MPCs, mesengenic and angiogenic potential have been extensively described [5][6][7][8][9] , but differentiation toward any other cells of mesodermal origin is still lacking. Thus, here we propose a new, and more rigorous, definition of these cells as "Mesangiogenic Progenitor Cells", maintaining the acronym MPCs.…”

Section: Representative Resultsmentioning

confidence: 99%

“…They retained both mesengenic and angiogenic potential and therefore were named Mesodermal Progenitor Cells (MPCs) 5 . Subsequently, we were able to define selective and reproducible culture conditions to generate MPCs at high grade of purity 6 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Montali

Barachini

Pacini

et al. 2016

JoVE

Self Cite

View full text Add to dashboard Cite

In a research study aimed to isolate human bone marrow (hBM)-derived Mesenchymal Stromal Cells (MSCs) for clinical applications, we identified a novel cell population specifically selected for growth in human serum supplemented medium. These cells are characterized by morphological, phenotypic, and molecular features distinct from MSCs and we named them Mesodermal Progenitor Cells (MPCs). MPCs are round, with a thick highly refringent core region; they show strong, trypsin resistant adherence to plastic. Failure to expand MPCs directly revealed that they are slow in cycling. This is as also suggested by Ki-67 negativity. On the other hand, culturing MPCs in standard medium designed for MSC expansion, gave rise to a population of exponentially growing MSC-like cells. Besides showing mesenchymal differentiation capacity MPCs retained angiogenic potential, confirming their multiple lineage progenitor nature. Here we describe an optimized highly reproducible protocol to isolate and characterize hBM-MPCs by flow cytometry (CD73, CD90, CD31, and CD45), nestin expression, and F-actin organization. Protocols for mesengenic and angiogenic differentiation of MPCs are also provided. Here we also suggest a more appropriate nomenclature for these cells, which has been re-named as "Mesangiogenic Progenitor Cells".

show abstract

Section: Representative Resultsmentioning

confidence: 99%

Section: Representative Resultsmentioning

confidence: 99%

Section: Representative Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Montali

Barachini

Pacini

et al. 2016

JoVE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other investigators recommend the cultivation in autologous human serum allowing a higher purification of the CB-MSC population [68].…”

Section: Embryonic Sc (Esc) Trans-differentiationmentioning

confidence: 99%

Cord Blood—An Alternative Source for Bone Regeneration

Jäger

Zilkens

Bittersohl

et al. 2009

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Bone regeneration is one of the best investigated pathways in mesenchymal stromal cell (MSC) biology. Therefore strong efforts have been made to introduce tissue engineering and cell therapeutics as an alternative treatment option for patients with bone defects. This review of the literature gives an overview of MSC biology aiming for clinical application including advantages but also specific challenges and problems which are associated with cord blood derived stromal cell (CB-MSC) as a source for bone regeneration. The use of postnatal CB-MSC is ethically uncomplicated and requires no invasive harvesting procedure. Moreover, most data document a high osteogenic potential of CB-MCS and also low immunoreactivity compared with other MSC types. The expression profile of CB-MSC during osteogenic differentiation shows similarities to that of other MSC types. Within the umbilical cord different MSC types have been characterized which are potent to differentiate into osteoblasts. In contrast to a large number of in vitro investigations there are only few in vivo studies available so far.

show abstract

HOX and TALE signatures specify human stromal stem cell populations from different sources

Picchi

Trombi

Spugnesi

et al. 2012

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

Human stromal stem cell populations reside in different tissues and anatomical sites, however a critical question related to their efficient use in regenerative medicine is whether they exhibit equivalent biological properties. Here, we compared cellular and molecular characteristics of stromal stem cells derived from the bone marrow, at different body sites (iliac crest, sternum, and vertebrae) and other tissues (dental pulp and colon). In particular, we investigated whether homeobox genes of the HOX and TALE subfamilies might provide suitable markers to identify distinct stromal cell populations, as HOX proteins control cell positional identity and, together with their co-factors TALE, are involved in orchestrating differentiation of adult tissues. Our results show that stromal populations from different sources, although immunophenotypically similar, display distinct HOX and TALE signatures, as well as different growth and differentiation abilities. Stromal stem cells from different tissues are characterized by specific HOX profiles, differing in the number and type of active genes, as well as in their level of expression. Conversely, bone marrow-derived cell populations can be essentially distinguished for the expression levels of specific HOX members, strongly suggesting that quantitative differences in HOX activity may be crucial. Taken together, our data indicate that the HOX and TALE profiles provide positional, embryological and hierarchical identity of human stromal stem cells. Furthermore, our data suggest that cell populations derived from different body sites may not represent equivalent cell sources for cell-based therapeutical strategies for regeneration and repair of specific tissues.

show abstract

Selective Culture of Mesodermal Progenitor Cells

Cited by 27 publications

References 11 publications

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Cord Blood—An Alternative Source for Bone Regeneration

HOX and TALE signatures specify human stromal stem cell populations from different sources

Contact Info

Product

Resources

About