Selective concentration of eicosapentaenoic acid and docosahexaenoic acid from fish oil with immobilized/stabilized preparations of Rhizopus oryzae lipase

Ashjari, Maryam; Mohammadi, Mehdi; Badri, Rashid

doi:10.1016/j.molcatb.2015.08.017

Cited by 26 publications

(16 citation statements)

References 41 publications

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“…Another study on TLL only achieved 26% after five cycles at 24 hr (Akanbi et al, ). This is comparable to the results obtained by Ashjari, Mohammadi, and Badri () who reused derivatives of free Rhizopus oryzae lipase (ROL) after recycling five times to achieve 67% hydrolysis. Nevertheless, due to the lower substrate availability, the DHA was hydrolyzed in the second round of hydrolysis, resulting in increased ω‐3 bova contained in the FFA fraction after refined hydrolysis (Cain, Harris, Moore, & McNeill, ).…”

Section: Discussionsupporting

confidence: 85%

Thermomyces lanuginosus lipase immobilized on magnetic nanoparticles and its application in the hydrolysis of fish oil

2018

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This article describes the immobilization and stabilization of lipase from Thermomyces lanuginosus (TLL) onto magnetic nanoparticles, which led to a markedly improved performance of the enzyme. The optimal immobilization conditions were found to be a pH of 7.5, 40°C, 4 hr, and 4.4 mg TLL protein/g of support of lipase. These results were used to enrich the docosahexaenoic acid (DHA) which is used in the fields of medicine and food. When the immobilized lipase was utilized to enrich DHA, the DHA content increased with the increasing amount of enzyme up to 4.4 mg TLL protein/g of support of lipase. The water content also influenced the DHA content, with the highest DHA content at 50% water, 40°C, and a pH of 7 after 14 hr that is, 4.4‐fold the initial content of DHA fish oil for TLL‐GMNPs. After six cycles of successive usage, it still remained over 82% relative hydrolysis. Practical applications One of the most important applications in use Omega 3, it is important for human health, industrial applications in the fields of food and medicine.

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Section: Discussionsupporting

confidence: 85%

Thermomyces lanuginosus lipase immobilized on magnetic nanoparticles and its application in the hydrolysis of fish oil

2018

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“…Nevertheless, the enzyme selectivity in discrimination between EPA and DHA depends on the lipase source and its functional properties. Although there are a few reports of poor selectivity of some lipases, most studies have reported high selectivity of lipases in releasing EPA and DHA in favor of EPA [16,17]. We have also reported the same selectivity in our previous studies of using Rhizomucor miehei lipase in selective hydrolysis of fish oil [18,19].…”

Section: Introductionsupporting

confidence: 79%

“…It has also been reported that small changes during immobilization strategy can alter the selectivity and activity of the immobilized enzyme. For example, the modulation of lipases selectivity in fish oil hydrolysis has been reported by oriented immobilization [16]. Therefore, the selectivity of the immobilized derivatives of RML was examined in selective hydrolysis of fish oil in order to evaluate the effect of different immobilization strategies on the selectivity of RML.…”

Section: Fish Oil Hydrolysismentioning

confidence: 99%

Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil

et al. 2020

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Functional properties of each enzyme strictly depend on immobilization protocol used for linking enzyme and carrier. Different strategies were applied to prepare the immobilized derivatives of Rhizomucor miehei lipase (RML) and chemically aminated RML (NH2-RML). Both RML and NH2-RML forms were covalently immobilized on glyoxyl sepharose (Gx-RML and Gx-NH2-RML), glyoxyl sepharose dithiothreitol (Gx-DTT-RML and Gx-DTT-NH2-RML), activated sepharose with cyanogen bromide (CNBr-RML and CNBr-NH2-RML) and heterofunctional epoxy support partially modified with iminodiacetic acid (epoxy-IDA-RML and epoxy-IDA-NH2-RML). Immobilization varied from 11% up to 88% yields producing specific activities ranging from 0.5 up to 1.9 UI/mg. Great improvement in thermal stability for Gx-DTT-NH2-RML and epoxy-IDA-NH2-RML derivatives was obtained by retaining 49% and 37% of their initial activities at 70 °C, respectively. The regioselectivity of each derivative was also examined in hydrolysis of fish oil at three different conditions. All the derivatives were selective between cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) in favor of EPA. The highest selectivity (32.9 folds) was observed for epoxy-IDA-NH2-RML derivative in the hydrolysis reaction performed at pH 5 and 4 °C. Recyclability study showed good capability of the immobilized biocatalysts to be used repeatedly, retaining 50–91% of their initial activities after five cycles of the reaction.

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“…The above materials have large pores or cavities where the enzymes can be housed. However, it is usually necessary to modify the surface of support to achieve a better anchorage by functionalization with amine, chloride, sulfur or phenol groups [10,13,[16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a biorefinery since biodiesel production could be performed without the energy-intensive step of biomass drying.

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Selective concentration of eicosapentaenoic acid and docosahexaenoic acid from fish oil with immobilized/stabilized preparations of Rhizopus oryzae lipase

Cited by 26 publications

References 41 publications

Thermomyces lanuginosus lipase immobilized on magnetic nanoparticles and its application in the hydrolysis of fish oil

Thermomyces lanuginosus lipase immobilized on magnetic nanoparticles and its application in the hydrolysis of fish oil

Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

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