Selective colorimetric urine glucose detection by paper sensor functionalized with polyaniline nanoparticles and cell membrane

Lee, Taeha; Kim, Insu; Cheong, Da Yeon; Roh, Seokbeom; Jung, Hyo Gi; Lee, Sang Won; Kim, Hyun Soo; Yoon, Dae Sung; Hong, Yoochan; Lee, Gyudo

doi:10.1016/j.aca.2021.338387

Cited by 33 publications

(29 citation statements)

References 55 publications

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“…Next, we plotted the absorbance ratio (λ 600 /λ 430 ) of PAni-NPs vs. the pH of the solution ranging from 3 to 9 ( Figure 2 b). An excellent linearity is observed, particularly at pH 5–8 (as shown in the inset of Figure 2 b), which is consistent with the results of our previous study [ 22 ]. It should be noted that the linear range of PAni-NPs for the colorimetric response is sufficient for various biomedical applications.…”

Section: Resultssupporting

confidence: 92%

“…PAni-NPs were synthesized using previously reported methods, with a few modifications [ 22 ]. A 25 mL APS solution at a 91.2 mg/mL concentration was added dropwise to a 60 mL solution containing 1.80 g pectin, 9 mL HCl (11 N), and 0.90 g aniline.…”

Section: Methodsmentioning

confidence: 99%

“…Although PAni-NPs have a green color at low pH, they exhibit a blue color at higher pH [ 20 ]. Based on this unique colorimetric response, PAni-NPs have been widely used to monitor changes in the pH of the surrounding environment [ 21 , 22 , 23 , 24 , 25 ]. In addition, it serves as a suitable matrix for biomolecular immobilization and promotes electron transport during redox reactions [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Lee

Kim

et al. 2022

Nanomaterials

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Hydrogels containing colorimetric nanoparticles have been used for ion sensing, glucose detection, and microbial metabolite analyses. In particular, the rapid chemical reaction owing to both the hydrogel form of water retention and the sensitive color change of nanoparticles enables the rapid detection of target substances. Despite this advantage, the poor dispersibility of nanoparticles and the mechanical strength of nanoparticle–hydrogel complexes have limited their application. In this study, we demonstrate a milliliter agarose gel containing homogeneously synthesized polyaniline nanoparticles (PAni-NPs), referred to as PAni-NP–hydrogel complexes (PNHCs). To fabricate the optimal PNHC, we tested various pH solvents based on distilled water and phosphate-buffered saline and studied the colorimetric response of the PNHC with thickness. The colorimetric response of the prepared PNHC to the changes in the pH of the solution demonstrated excellent linearity, suggesting the possibility of using PNHC as a pH sensor. In addition, it was verified that the PNHC could detect minute pH changes caused by the cancer cell metabolites without cytotoxicity. Furthermore, the PNHC can be stably maintained outside water for approximately 12 h without deformation, indicating that it can be used as a disposable patch-type wearable biosensing platform.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Lee

Kim

et al. 2022

Nanomaterials

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“…In a previous study, we analyzed the thickness of the RBCM per unit area according to the amount of RBCM. [ 39 ] In the case of the vesicle fusion method, the RBCM membrane was designed to have a thickness of approximately 400 nm to achieve sufficient mechanical strength, which is equivalent to 80 cell membranes. Therefore, the total thickness of the GO–GRGDS–AQP (vesicle fusion coating) membrane was estimated to be ≈700 nm, while that of the GO–GRGDS–AQP (interlayer) was 611 nm, as shown in Figure S4b , Supporting Information.…”

Section: Resultsmentioning

confidence: 99%

Aquaporin‐Incorporated Graphene‐Oxide Membrane for Pressurized Desalination with Superior Integrity Enabled by Molecular Recognition

et al. 2021

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Aquaporins (AQPs), the natural water channel, have been actively investigated for overcoming the limitations of conventional desalination membranes. An AQP-based biomimetic high-pressure desalination membrane is designed by tethering AQP-carrying red blood cell membrane (RBCM) vesicles onto graphene oxide (GO). RBCMs with AQPs are incorporated into GO based on the molecular recognition between the integrin of RBCM and Arginine-Glycine-Aspartate (RGD) ligand on the GO surface. GO is pre-functionalized with the Glycine-Arginine-Glycine-Aspartate-Serine peptide to capture RBCMs. RBCMs are inserted between GO flakes through the material-specific interaction between integrin of RBCM and RGD ligand, thus ensuring sufficient coverage of channels/defects in the GO for the full functioning of the AQPs. The incorporated AQPs are not completely fixed at the GO, as tethering is mediated by the integrin-RGD pair, and suitable AQP flexibility for appropriate functioning is guaranteed without frictional hindrance from the solid substrate. The integrity of the GO-RBCMs binding can provide mechanical strength for enduring high-pressure reverse-osmosis conditions for treating large amounts of water. This biomimetic membrane exhibits 99.1% NaCl rejection and a water permeance of 7.83 L m −2 h −1 bar −1 at 8 bar with a 1000-ppm NaCl feed solution, which surpasses the upper-bound line of current state-of-the-art membranes.

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“…Many lab-on-paper platforms with integrated colorimetric detection [97][98][99][100][101] have been proposed for determining the glucose concentration in human urine samples. de Oliveira et al [102] developed 2D and 3D lab-on-paper platforms for the integrated colorimetric detection of glucose, total protein and nitrite in serum and artificial urine.…”

Section: Glucose Analysismentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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In recent years, microfluidic lab-on-paper devices have emerged as a rapid and low-cost alternative to traditional laboratory tests. Additionally, they were widely considered as a promising solution for point-of-care testing (POCT) at home or regions that lack medical infrastructure and resources. This review describes important advances in microfluidic lab-on-paper diagnostics for human health monitoring and disease diagnosis over the past five years. The review commenced by explaining the choice of paper, fabrication methods, and detection techniques to realize microfluidic lab-on-paper devices. Then, the sample pretreatment procedure used to improve the detection performance of lab-on-paper devices was introduced. Furthermore, an in-depth review of lab-on-paper devices for disease measurement based on an analysis of urine samples was presented. The review concludes with the potential challenges that the future development of commercial microfluidic lab-on-paper platforms for human disease detection would face.

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Selective colorimetric urine glucose detection by paper sensor functionalized with polyaniline nanoparticles and cell membrane

Cited by 33 publications

References 55 publications

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Colorimetric Nanoparticle-Embedded Hydrogels for a Biosensing Platform

Aquaporin‐Incorporated Graphene‐Oxide Membrane for Pressurized Desalination with Superior Integrity Enabled by Molecular Recognition

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

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