Selective Changes in White Cell Lysosomal Enzymes in Man*

Jg, Crowder; White, A. Clinton

doi:10.1097/00000441-196805000-00008

Cited by 17 publications

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“…Perrillie has suggested that serum muramidase is derived principally from the degeneration of mature granulocytes and mono cytes [7]. Many workers have found the value to be elevated in diseases which have an increased production of granulocytes or monocytes [5,6,7,8],…”

Section: Discussionmentioning

confidence: 99%

“…In adults, who have a more predictable granulocyte response serum muramidase is indeed increas ed during bacterial infections [6].…”

Section: Discussionmentioning

confidence: 99%

“…

Serum muramidase may be considered a rough index of leukopoiesis both in the normal state [1,2,3,4] and in proliferative diseases involving myeloid and monocytic cells [5,6,7,8], Since the blood of the neonate differs from that of the normal adult in certain metabolic pathways and in the variability of its white cell population, serum muramidase was investigated in this age group.

Method and M aterialScrum muramidase activity was measured by a modified turbidimctric method of Sm o lelis and H a r t s e l l [9,10], Point two ml aliquots of serum were added to 2 ml of standard egg-white muramidase substrate. The changes in optical density were measured in duplicate at 30-second intervals for 2 min in a Coleman spectrophotometer.

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confidence: 99%

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Serum Muramidase in Newborns

McMahon¹,

Zanger²,

Desforges³

1971

Neonatology

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Section: Discussionmentioning

confidence: 99%

“…In adults, who have a more predictable granulocyte response serum muramidase is indeed increas ed during bacterial infections [6].…”

Section: Discussionmentioning

confidence: 99%

“…

…”

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Serum Muramidase in Newborns

McMahon¹,

Zanger²,

Desforges³

1971

Neonatology

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“…The supernatant was assayed for muramidase activity by a method similar to that described by Litwack (1955). The content of rnurarnidase in peripheral neutrophils was measured by the method of Crowder and White (1968).…”

Section: Methodsmentioning

confidence: 99%

The effect of bilateral nephrectomy on the production and distribution of muramidase (lysozyme) in the rat

Keeler¹

1970

Can. J. Physiol. Pharmacol.

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KEELER, Pa. 1970. The effect of bilateral nephrectomy on the production and distribution of muramidase (lymzyme) in the rat. Can. J. Physiol. Pharmacol. 48, 131-1323, After bilateral nephrectomy total body muramidase in the rat increased by more than 50%. The mean rate of increase was 340 pg/h per 100 g. Skin and bone showed the greatest increase. Although the Bungs contain a high level of muramidase there was no significant change in the level of enzyme activity after nephrectomy. Rats with sectioned ureters or with a ureterovenous anastomosis did not develop high plasma Bevels of muramidase. The response to nephrectomy is therefore not a response to uremia. Pretreatment of the animals with cortisone or DL-ethionine had no effect on the enzyme response to nephrectorny. This was taken to indicate that the response was not to antigenic material and did not depend upon hepatic synthesis of the enzyme. Pretreatment with the cytotoxic agent cyclophosphamide reduced the plasma and total body Bevel of muran~idase and blocked the response to nephrectomy. Direct measurement demonstrated a,large increase in the rate of murarnidase production by bone marrow. Since the in vitro inactivation sf murarnidase by kidney tissue could not be demonstrated, it is concluded that nephrectomy causes an increase in the rate of enzyme production rather than a failure of catabolism. Renal tissue might normally exert an inhibitory influence upon muramidase foranation.

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“…It is absent from lymphocytes (Barnes, 1940) but is present in monocytes and macrophages (Myrvik et al, 1961). That it is also derived from granulocytes seems likely since the highest serum levels are found in monocytic and myelomonocytic leukaemia, and there is some elevation in all myelogenous leukaemia and during the course of bacterial infections (Crowder and White, 1968). Probably the lysozyme present in normal peritoneal fluid is partly derived from the serum and partly from white cells resident around the peritoneum.…”

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confidence: 99%