Selective block of tunneling nanotube (TNT) formation inhibits intercellular organelle transfer between PC12 cells

Bukoreshtliev, Nickolay V.; Wang, Xiang; Hodneland, Erlend; Gurke, Steffen; Barroso, João Filipe; Gerdes, Hans‐Hermann

doi:10.1016/j.febslet.2009.03.065

Cited by 189 publications

(207 citation statements)

References 24 publications

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“…S5). Nanotube formation is dependent on actin polymerization (Gurke et al, 2008;Bukoreshtliev et al, 2009). Treatment of the co-cultures with the inhibitor of actin polymerization latrunculin-A led to a decrease in vesicle transfer and completely abrogated the effect of LST1 overexpression on the transfer rate ( Fig.…”

Section: Lst1 Supports the Formation Of Functional Tunneling Nanotubesmentioning

confidence: 99%

LST1 promotes the assembly of a molecular machinery responsible for tunneling nanotube formation

Schiller

Diakopoulos

Rohwedder

et al. 2012

Journal of Cell Science

108

125

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SummaryCarefully orchestrated intercellular communication is an essential prerequisite for the development of multicellular organisms. In recent years, tunneling nanotubes (TNT) have emerged as a novel and widespread mechanism of cell-cell communication. However, the molecular basis of their formation is still poorly understood. In the present study we report that the transmembrane MHC class III protein leukocyte specific transcript 1 (LST1) induces the formation of functional nanotubes and is required for endogenous nanotube generation. Mechanistically, we found that LST1 induces nanotube formation by recruiting the small GTPase RalA to the plasma membrane and promoting its interaction with the exocyst complex. Furthermore, we determined that LST1 recruits the actincrosslinking protein filamin to the plasma membrane and interacts with M-Sec, myosin and myoferlin. These results allow us to suggest a molecular model for nanotube generation. In this proposal LST1 functions as a membrane scaffold mediating the assembly of a multimolecular complex, which controls the formation of functional nanotubes.

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Section: Lst1 Supports the Formation Of Functional Tunneling Nanotubesmentioning

confidence: 99%

LST1 promotes the assembly of a molecular machinery responsible for tunneling nanotube formation

Schiller

Diakopoulos

Rohwedder

et al. 2012

Journal of Cell Science

108

125

View full text Add to dashboard Cite

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“…The organelles can be disrupted by mechanical stress, chemical fixation, and even long-term light exposure [12]. Latrunculin B and Cytocalasin D could inhibit the formation of MNTs [17,23] and prevent the polymerization of actin filaments by binding to actin monomers or capping the F-actin plus end. Therefore, F-actin might have an important role in the formation of MNTs and may regulate MNT formation by affecting cytoskeleton remodeling.…”

Section: Mechanisms Involved In the Formation Of Mntsmentioning

confidence: 99%

“…Indeed, mobile cells, such as normal rat kidney and T cells, which easily connect with other cells, form tubes by the first method [15,16]. Most immobile cell types, such as PC12, form tubes by use of an outgrowth [17].…”

Section: Formation Modes Of Mntsmentioning

confidence: 99%

Membrane nanotubes: Novel communication between distant cells

Zhang

2013

Sci. China Life Sci.

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The many kinds of cell structures involved in cell-cell communication include tight junction, adherens junction and gap junction, but almost all are between adjacent cells. Recently, a general and dynamic membrane tether, termed tunneling nanotubes or membrane nanotubes (MNTs), was discovered to be involved in communication between distant cells. By facilitating intercellular communication, MNTs contribute to many biological functions and pathologic changes in cells. Many works have revealed the structure, formation and functional properties of MNTs. However, as novel structures, further research is needed.

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“…Although the mechanisms of their formation do appear to be different from filopodia, TNTs may however, arise from filopodialike structures. 9,21,22 We therefore addressed whether overexpression of PrP C also affected TNT formation, and performed similar TNTcounting and transfer experiments after overexpression of GFP-PrP C .…”

Section: Prp C Levels Affect Tnt Formationmentioning

confidence: 99%

Prion aggregates transfer through tunneling nanotubes in endocytic vesicles

Zhu

Victoria

Marzo

et al. 2015

Prion

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ABSTRACT. Transmissible spongiform encephalopathies (TSEs) are a group of neurodegenerative diseases caused by the misfolding of the cellular prion protein to an infectious form PrP Sc . The intercellular transfer of PrP Sc is a question of immediate interest as the cell-to-cell movement of the infectious particle causes the inexorable propagation of disease. We have previously identified tunneling nanotubes (TNTs) as one mechanism by which PrP Sc can move between cells. Here we investigate further the details of this mechanism and show that PrP Sc travels within TNTs in endolysosomal vesicles. Additionally we show that prion infection of CAD cells increases both the number of TNTs and intercellular transfer of membranous vesicles, thereby possibly playing an active role in its own intercellular transfer via TNTs.

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Selective block of tunneling nanotube (TNT) formation inhibits intercellular organelle transfer between PC12 cells

Cited by 189 publications

References 24 publications

LST1 promotes the assembly of a molecular machinery responsible for tunneling nanotube formation

LST1 promotes the assembly of a molecular machinery responsible for tunneling nanotube formation

Membrane nanotubes: Novel communication between distant cells

Prion aggregates transfer through tunneling nanotubes in endocytic vesicles

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