Selective biodegradation of recalcitrant black chicken feathers by a newly isolated thermotolerant bacterium Pseudochrobactrum sp. IY-BUK1 for enhanced production of keratinase and protein-rich hydrolysates

Ibrahim, Yusuf; Garba, Lawal; Shehu, Mustapha Ahmad; Oyiza, Aminat Musa; Kabir, Muhammad Rabiu; Haruna, Musa

doi:10.1007/s10123-019-00090-4

Cited by 14 publications

(11 citation statements)

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“…The addition of extra sucrose, glucose, maltose, and mannose suppressed the keratinase activity and degradation of heavy-metal-laden GFFs. This contradicts the previous report in which the strain's keratinase yield was stimulated by sucrose in the degradation of melanized chicken feathers (Yusuf et al, 2020). This suggests that the strain's nutritional requirements vary from one substrate to another.…”

Section: Optimization Of Parameters Affecting the Ability Of The Bact...contrasting

confidence: 91%

“…The morphological, biochemical, and 16S rRNA sequence analyses of the selected bacterium (Pseudochrobactrum sp. IY-BUK1) have been reported in a previous study (Yusuf et al, 2020). The bacterium, which is Gram-negative, oxidase-positive, non-spore-forming, and nonmotile bacilli, was the first GFF-DB to be reported.…”

Section: Resultsmentioning

confidence: 84%

“…The supernatant from each tube was inoculated in a 250 ml Erlenmeyer flask containing 100 ml feather meal broth (FMB) and supplemented with GFFs as a sole source of carbon and nitrogen. FMB was prepared as described earlier by Yusuf et al (2016Yusuf et al ( , 2019Yusuf et al ( , 2020) by dissolving 0.5 g/l of NaCl, 0.7 g/l of K 2 HPO 4 , 1.4 g/l of KH 2 PO 4 , and 0.001 g/l of MgSO 4 @ 6H 2 O in 1000 ml of distilled water, and the pH of the mixture was adjusted to 7.5. The inoculated FMB were incubated on an orbital shaker operating at 150 rpm at 30EC.…”

Section: Isolation Of Gff-degrading Bacteria (Gff-db) and Preparation...mentioning

confidence: 99%

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Bioremediation of Cu and Zn by guinea fowl feather wastes and biodegradation of Cu- and Zn-polluted feathers by free and immobilized heavy-metal-tolerant bacterium Pseudochrobactrum sp. IY-BUK1

Yusuf¹,

Sharu²

2022

bta

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One of the major pollutants generated from guinea fowl farms and slaughterhouses is guinea fowl feather (GFF) wastes. Chicken feathers are an important substrate for removing aqueous pollutants due to their structural characteristics, but the application of GFFs for such purposes has not been elucidated. This study was carried out to determine the potential of raw as well as chemically and physically pretreated GFFs in the bioremediation of copper (Cu) and zinc (Zn) from aqueous solutions and the subsequent biodegradation of Cu-and Zn-laden GFFs using free and immobilized heavy-metal-tolerant bacteria. Using atomic adsorption spectrometry, the ability of treated and untreated GFFs to adsorb Cu and Zn was determined, and the generated heavy-metal-laden GFFs were degraded using Pseudochrobactrum sp. IY-BUK1. The results revealed that under optimized conditions, acetone and autoclave pretreatment enhanced Cu and Zn removal by 40% within 6 h when compared with raw GFFs. Similarly, complete biodegradation of Cu-and Zn-laden GFFs was achieved at pH 8, with 2% inoculum size, and at 25EC using IY-BUK1 in 7 days. Upon optimization of physical and nutritional conditions, using one factor at a time and response surface methodology, the maximum keratinase activity was achieved at 30EC, with 3% inoculum size and 3.5% polluted GFF concentration in 3 h. The degradation and keratinase activity were further enhanced by 50% by the immobilization of Pseudochrobactrum sp. IY-BUK1 cells compared with free cells. Thus, GFFs can act as good biosorbents, and together with IY-BUK1, the use of GFFs can be an alternative approach to dispose of GFFs, thus preventing environmental pollution via bioremediation.

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Section: Optimization Of Parameters Affecting the Ability Of The Bact...contrasting

confidence: 91%

Section: Resultsmentioning

confidence: 84%

Section: Isolation Of Gff-degrading Bacteria (Gff-db) and Preparation...mentioning

confidence: 99%

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Bioremediation of Cu and Zn by guinea fowl feather wastes and biodegradation of Cu- and Zn-polluted feathers by free and immobilized heavy-metal-tolerant bacterium Pseudochrobactrum sp. IY-BUK1

Yusuf¹,

Sharu²

2022

bta

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“…Previous studies have reported that Pseudochrobactrum sp. IY-BUK1 produces enhanced keratinase and protein-rich hydrolysates and has the potential to be used in chicken feather biodegradation [ 42 ]. Adding probiotics, such as Enterobacter sp., to the diet could change the bacterial load of Enterobacteriaceae in the intestinal tract of Bactrocera cucurbitae , reduce the abundance of Pseudomonas and significantly improve the quality control parameters of the flies [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Enterobacter hormaechei in the intestines of housefly larvae promotes host growth by inhibiting harmful intestinal bacteria

et al. 2021

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Background As a pervasive insect that transmits a variety of pathogens to humans and animals, the housefly has abundant and diverse microbial communities in its intestines. These gut microbes play an important role in the biology of insects and form a symbiotic relationship with the host insect. Alterations in the structure of the gut microbial community would affect larval development. Therefore, it is important to understand the mechanism regulating the influence of specific bacteria on the development of housefly larvae. Methods For this study we selected the intestinal symbiotic bacterium Enterobacter hormaechei, which is beneficial to the growth and development of housefly larvae, and used it as a probiotic supplement in larval feed. 16S rRNA gene sequencing technology was used to explore the effect of E. hormaechei on the intestinal flora of housefly larvae, and plate confrontation experiments were performed to study the interaction between E. hormaechei and intestinal microorganisms. Results The composition of the gut microflora of the larvae changed after the larvae were fed E. hormaechei, with the abundance of Pseudochrobactrum, Enterobacter and Vagococcus increasing and that of Klebsiella and Bacillus decreasing. Analysis of the structure and interaction of larval intestinal flora revealed that E. hormaechei inhibited the growth of harmful bacteria, such as Pseudomonas aeruginosa, Providencia stuartii and Providencia vermicola, and promoted the reproduction of beneficial bacteria. Conclusions Our study has explored the influence of specific beneficial bacteria on the intestinal flora of houseflies. The results of this study reveal the important role played by specific beneficial bacteria on the development of housefly larvae and provide insight for the development of sustained biological agents for housefly control through interference of gut microbiota. Graphical abstract

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“…KMC2 in KMCG6. Representatives from these three genera have been reported with keratinolytic activity toward feather keratin (Tamreihao et al, 2019;Yusuf et al, 2019). Nevertheless, the roles of these different microbes in KMCG6 were still not clear.…”

Section: Presentation Of the Initial Microbial Consortium Kmcg6mentioning

confidence: 99%

Construction of Simplified Microbial Consortia to Degrade Recalcitrant Materials Based on Enrichment and Dilution-to-Extinction Cultures

et al. 2020

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The capacity of microbes to degrade recalcitrant materials has been extensively explored for environmental remediation and industrial production. Significant achievements have been made with single strains, but focus is now going toward the use of microbial consortia owning to their functional stability and efficiency. However, assembly of simplified microbial consortia (SMC) from complex environmental communities is still far from trivial due to large diversity and the effect of biotic interactions. Here we propose a strategy, based on enrichment and dilution-to-extinction cultures, to construct SMC with reduced diversity for degradation of keratinous materials. Serial dilutions were performed on a keratinolytic microbial consortium preenriched from a soil sample, monitoring the dilution effect on community growth and enzymatic activities. An appropriate dilution regime (10 −9) was selected to construct a SMC library from the enriched microbial consortium. Further sequencing analysis and keratinolytic activity assays demonstrated that obtained SMC displayed actual reduced microbial diversity, together with various taxonomic composition, and biodegradation capabilities. More importantly, several SMC possessed equivalent levels of keratinolytic efficiency compared to the initial consortium, showing that simplification can be achieved without loss of function and efficiency. This methodology is also applicable to other types of recalcitrant material degradation involving microbial consortia, thus considerably broadening its application scope.

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Selective biodegradation of recalcitrant black chicken feathers by a newly isolated thermotolerant bacterium Pseudochrobactrum sp. IY-BUK1 for enhanced production of keratinase and protein-rich hydrolysates

Cited by 14 publications

References 50 publications

Bioremediation of Cu and Zn by guinea fowl feather wastes and biodegradation of Cu- and Zn-polluted feathers by free and immobilized heavy-metal-tolerant bacterium Pseudochrobactrum sp. IY-BUK1

Bioremediation of Cu and Zn by guinea fowl feather wastes and biodegradation of Cu- and Zn-polluted feathers by free and immobilized heavy-metal-tolerant bacterium Pseudochrobactrum sp. IY-BUK1

Enterobacter hormaechei in the intestines of housefly larvae promotes host growth by inhibiting harmful intestinal bacteria

Construction of Simplified Microbial Consortia to Degrade Recalcitrant Materials Based on Enrichment and Dilution-to-Extinction Cultures

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