Selective binding of soybean agglutinin to the olfactory system of Xenopus

Key, Brian; Giorgi, P. P.

doi:10.1016/0306-4522(86)90171-5

Cited by 74 publications

(42 citation statements)

References 33 publications

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“…Although the functional significance of the vomeronasal epithelium in amphibians remains unclear, these previous studies strongly suggest the presence of three types of distinct olfactory subsystems in the Xenopus primary olfactory system. Several authors reported differential stainings of one lectin, SBA, among the three kinds of the neuroepithelia and the axons emerging from each of them, and suggested the presence of three olfactory pathways in the Xenopus primary olfactory system [12,17,19,21,22]. This suggestion represents that each of the three distinct olfactory subsystems constitutes the unique projection pathway from the epithelium to the olfactory bulb, and that each olfactory pathway differs in its expression of glycoconjugates.…”

Section: Discussionmentioning

confidence: 99%

“…Föske [9] first described this middle chamber epithelium in Xenopus laevis, and Altner [1] reported that a valve-like skin flop in the nostril closes the PC and opens the MC to adapt to the aquatic environment when the frog dives. Key and Giorgi [19] applied lectinhistochemistry using soybean agglutinin (SBA), and reported the SBA-positive subset in the Xenopus main olfactory system. Thereafter, it has been suggested that the SBApositive subset arises from the MC and terminates in the ventral region of the MOB, and its function may differ from the SBA-negative subset arising from the PC [12,17,19,21,22].…”

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confidence: 99%

“…Key and Giorgi [19] applied lectinhistochemistry using soybean agglutinin (SBA), and reported the SBA-positive subset in the Xenopus main olfactory system. Thereafter, it has been suggested that the SBApositive subset arises from the MC and terminates in the ventral region of the MOB, and its function may differ from the SBA-negative subset arising from the PC [12,17,19,21,22]. These previous studies strongly suggest the presence of three distinct olfactory pathways in Xenopus laevis, although there are almost no morphological data on the differences in chemical properties among these three olfactory pathways.…”

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confidence: 99%

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Expression Patterns of Glycoconjugates in the Three Distinctive Olfactory Pathways of the Clawed Frog, Xenopus laevis.

Saito

Taniguchi

2000

The Journal of Veterinary Medical Science

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Expression Patterns of Glycoconjugates in the Three Distinctive Olfactory Pathways of the Clawed Frog, Xenopus laevis.

Saito

Taniguchi

2000

The Journal of Veterinary Medical Science

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“…However, independent of methodological differences, reasonable numbers of SBA binding neurones have been demonstrated in the mammalian brain. In contrast to the situation in mammals, SBA labels the surface of only a (sub)population of olfactory neurones in eels (Franceschini and Ciani, 1991) and frogs (Hofmann and Meyer, 1991;Key and Giorgi, 1986), but does not label other cells in the central nervous system of these animals. In mice it has been shown that viciu uillosa agglutinin, which labels essentially the same cells as SBA (Kosaka and Heizmann, 19891, fails to produce the typical labelling pattern in 1 week old animals (Nakagawa et al, 1987).…”

Section: Discussionmentioning

confidence: 87%

“…The lectin from Giycine rnax (soybean agglutinin, SBA) was selected because it has been shown to selectively bind to N-acetylgalactosamine in other preparations of neuronal tissue (Nakagawa et al, 1986;Hofman and Meyer, 1991;Key and Giorgi, 1986). Endogenous peroxidase was inactivated by treatment in 0.5% H,O, in 0.1 M, pH 7.4 sodium phosphate buffer for 30 min.…”

Section: Methodsmentioning

confidence: 99%

The distribution of N-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin

Gleich

1994

Hearing Research

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A horseradish peroxidase conjugated lectin from Glycine max (soy bean agglutinin; SBA) was used to characterise the distribution of N-acetylgalactosamine in the cochlear nucleus of the mongolian gerbil. SBA bound differentially to a variety of structures within the cochlear nucleus. Specific SBA labelling was associated with large non-granule neurones of variable size and shape throughout the cochlear nucleus. Compared to adjacent Nissl-stained sections 80% of the non-granule cells in the dorsal cochlear nucleus (DCN) and more than 90% of the non-granule cells in the ventral cochlear nucleus (VCN) bound SBA. The variation in location, size and shape as well as the high percentage of the labelled neurones suggest that cells of several, if not all, non-granule cell types, which have been described for the cochlear nucleus according to the usual Nissl schemes, are SBA positive. Granule cells did not bind SBA because all SBA-labelled cells had diameters above 10 pm. Diffuse labelling, not systematically associated with cells or fibres, was high in the molecular and fusiform cell layers of the DCN and that part of the granule cell area located close to the surface of the VCN. Darkly labelled granules (up to 2 pm diameter) were prominent in the area of the VIIIth nerve root. After long SBA incubations, they were also present in VCN and to a lesser degree in DCN. The results are discussed with respect to findings in other brain areas and the possible co-localisation of gamma aminobutyric acid (GABA), parvalbumin and N-acetylgalactosamine.

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N-acetyl-lactosamine in the rat olfactory system: Expression and potential role in neurite growth

Puche

Key

1996

J. Comp. Neurol.

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Primary sensory olfactory neurons exhibit a mosaic topographical projection from the olfactory neuroepithelium in the nasal cavity to the olfactory bulb form-ation of the telencephalon. Axons from primary neurons that are widely scattered in the epithelium terminate in discrete regions of the olfactory bulb. It has been hypothesised that carbohydrates present on the surface of primary olfactory axons mediate selective fasciculation and the formation of the topographical pathway. We examined the expression of the disaccharide N-acetyl-lactosamine in both the developing and the adult rat olfactory system. N-acetyl-lactosamine was expressed by all primary sensory olfactory neurons and by their terminations in the olfactory bulb throughout embryonic development and early postnatal life. In adults, N-acetyl-lactosamine was restricted to a subpopulation of primary sensory olfactory neurons that were dispersed throughout the neuroepithelium but that projected predominantly to the ventrolateral and ventromedial surfaces of the olfactory bulb. The axons of these neurons sort out in the outer layer of the bulb and preferentially self-fasciculate to form distinct axon bundles that terminate within select glomeruli. The role of N-acetyl-lactosamine in axon growth was tested by culturing primary sensory olfactory neurons on substrate-bound carbohydrates. Olfactory neuroepithelium cultures from both embryonic and postnatal rats revealed that substratebound N-acetyl-lactosamine was a strong and specific neurite growth-promoting agent. These data suggest that, during development of the olfactory projection, N-acetyl-lactosamine, which is present on all olfactory axons, acts as a nonselective permissive substrate for axon growth. In adults, however, the restricted distribution of N-acetyl-lactosamine on a subpopulation of axons may facilitate sorting out and self-fasciculation, which is necessary for preserving the mosaic nature of the olfactory pathway in this highly plastic region of the nervous system. These results support the hypothesis that cell surface carbohydrates are involved in axon growth in the olfactory system.

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Selective binding of soybean agglutinin to the olfactory system of Xenopus

Cited by 74 publications

References 33 publications

Expression Patterns of Glycoconjugates in the Three Distinctive Olfactory Pathways of the Clawed Frog, Xenopus laevis.

Expression Patterns of Glycoconjugates in the Three Distinctive Olfactory Pathways of the Clawed Frog, Xenopus laevis.

The distribution of N-acetylgalactosamine in the cochlear nucleus of the gerbil revealed by lectin binding with soybean agglutinin

N-acetyl-lactosamine in the rat olfactory system: Expression and potential role in neurite growth

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