Selective assembly of DNA using DLC film as passivation layer for the application to nano-device

Kim, Hyung Jin; Yun, Deok Yong; Choi, Won Seok; Hong, Byungyou

doi:10.1016/j.diamond.2009.02.027

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Prior to forming single DNA nanowire arrays on the APS coated region, we compared octadecyltrichlorosilane (OTS) to DLC in formation of single DNA nanowires as OTS is also known as a passivation coating reagent 22,23 . In our previous study, contact angle values for OTS-, DLC-and APS-coated surface were 95°, 75°and 55°, respectively 24 . Therefore, a DLC layer possesses hydrophobicity similar to an OTS layer.…”

Section: Resultsmentioning

confidence: 85%

Hybrid gold/DNA nanowire circuit with sub-10 nm nanostructure arrays

et al. 2020

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We report on a simple and efficient method for the selective positioning of Au/DNA hybrid nanocircuits using a sequential combination of electron-beam lithography (EBL), plasma ashing, and a molecular patterning process. The nanostructures produced by the EBL and ashing process could be uniformly formed over a 12.6 in2 substrate with sub-10 nm patterning with good pattern fidelity. In addition, DNA molecules were immobilized on the selectively nanopatterned regions by alternating surface coating procedures of 3-(aminopropyl)triethoxysilane (APS) and diamond like carbon (DLC), followed by deposition of DNA molecules into a well-defined single DNA nanowire. These single DNA nanowires were used not only for fabricating Au/DNA hybrid nanowires by the conjugation of Au nanoparticles with DNA, but also for the formation of Au/DNA hybrid nanocircuits. These nanocircuits prepared from Au/DNA hybrid nanowires demonstrate conductivities of up to 4.3 × 105 S/m in stable electrical performance. This selective and precise positioning method capable of controlling the size of nanostructures may find application in making sub-10 nm DNA wires and metal/DNA hybrid nanocircuits.

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Section: Resultsmentioning

confidence: 85%

Hybrid gold/DNA nanowire circuit with sub-10 nm nanostructure arrays

et al. 2020

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“…We investigated local roughness and waviness of threads by atomic-resolution TEM, and compared our findings with combing based on several results from the literature [6], [19], [20], [32], [33], [34], [35], [36], [37], [38], [39], [40] (see Figure 5 ). Threads are extremely straight both locally (<100 nm length) and over larger subunit lengths (>100 nm length).…”

Section: Discussionmentioning

confidence: 99%

Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface

Payne

Andregg²,

Kemmish³

et al. 2013

PLoS ONE

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We present “molecular threading”, a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and fluorescence and electron microscopies are used to characterize the angular distribution, straightness, and reproducibility of stretched DNA deposited in arrays onto elastomeric surfaces and thin membranes. Molecular threading demonstrates high straightness and uniformity over length scales from nanometers to micrometers, and represents an alternative to existing DNA deposition and linearization methods. These results point towards scalable and high-throughput precision manipulation of single-molecule polymers.

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“…In recent years, many combing methods have been developed for stretching DNA. Such methods include dynamic molecular combing (meniscus moving on a coverslip [14]), spin-stretching [8,9], the evaporation of small droplets of DNA solutions [15,16], the stretching of DNA with a cover slip [17], the mechanical movement of a *Corresponding author (email: 51888lyy@sina.com) meniscus [18], droplet motion driven by a nitrogen gas flow [19], the precise control of the meniscus motion [20], the ordered stretching of DNA between micro fabricated polystyrene lines [21], the moving droplet method [22] and aligning DNA molecules on a SiO 2 surface using a diamond-like carbon (DLC) thin film [23]. Combined with other techniques, the applications of the molecular combing method can be widened to include positioning genes on chromosomes [14], the genomic studies of DNA replication [24], the observation of transcription on a single combed DNA [25], the study of the interaction between DNA and enzymes [26,27] and the self-organized DNA network between two cover slips [28].…”

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confidence: 99%

Study of the interaction of DNA and histones by spin-stretching and droplet evaporation

et al. 2011

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Molecular combing is a powerful and simple method for aligning DNA molecules onto a surface. Using this technique combined with fluorescence microscopy, DNA-histone complexes are stretched on a hydrophobic polymethyl methacrylate (PMMA) surface and observed directly. We have developed a new method to stretch single DNA-histone complexes, termed spin-stretching. The results show that the histones markedly enhance DNA binding to the PMMA surface. DNA winds around the histones and therefore decreases in length. The number of histones that bind to each DNA molecule is found to correlate with the histone concentration. The combed DNA-histone complexes are found to depend on two factors: the binding force on the surface and the centrifugal force at its local position. Na + ions should compete with histones for binding to DNA; however, the observed competitive binding effect of Na + ions at low concentrations was negligible. Molecular combing is a novel approach for stretching DNA molecules. In recent years, many combing methods have been developed for stretching DNA. Such methods include dynamic molecular combing (meniscus moving on a coverslip [14]), spin-stretching [8,9], the evaporation of small droplets of DNA solutions [15,16], the stretching of DNA with a cover slip [17], the mechanical movement of a *Corresponding author (email: 51888lyy@sina.com) meniscus [18], droplet motion driven by a nitrogen gas flow [19], the precise control of the meniscus motion [20], the ordered stretching of DNA between micro fabricated polystyrene lines [21], the moving droplet method [22] and aligning DNA molecules on a SiO 2 surface using a diamond-like carbon (DLC) thin film [23]. Combined with other techniques, the applications of the molecular combing method can be widened to include positioning genes on chromosomes [14], the genomic studies of DNA replication [24], the observation of transcription on a single combed DNA [25], the study of the interaction between DNA and enzymes [26,27] and the self-organized DNA network between two cover slips [28].Eukaryotic genes do not exist as naked DNA molecules in the nucleus of a cell. Instead, they combine with particular proteins, especially the basic proteins called histones, to form a substance known as chromatin [29]. Chromatin controls gene activity and the inheritance of traits. The fun-

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Selective assembly of DNA using DLC film as passivation layer for the application to nano-device

Cited by 5 publications

References 34 publications

Hybrid gold/DNA nanowire circuit with sub-10 nm nanostructure arrays

Hybrid gold/DNA nanowire circuit with sub-10 nm nanostructure arrays

Molecular Threading: Mechanical Extraction, Stretching and Placement of DNA Molecules from a Liquid-Air Interface

Study of the interaction of DNA and histones by spin-stretching and droplet evaporation

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