Selective Array‐Based Sensing of Anabolic Steroids in Aqueous Solution by Host–Guest Reporter Complexes

Gill, Adam D.; Perez, Lizeth; Salinas, Isaac N. Q.; Byers, Samantha R.; Liu, Yang; Hickey, Briana L; Zhong, Wenwan; Hooley, Richard J.

doi:10.1002/chem.201804854

Cited by 17 publications

(18 citation statements)

References 51 publications

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“…Aggregation can be mediated by the presence of both metal ions and steroids. Both the “turn-on” and “turn-off” modes of fluorophores are essential for analyte discrimination [ 127 ].…”

Section: Figurementioning

confidence: 99%

Advances in the Determination of Anabolic-Androgenic Steroids: From Standard Practices to Tailor-Designed Multidisciplinary Approaches

Huml

Tauchen

Rimpelová

et al. 2021

Sensors

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Anabolic-androgenic steroids (AASs), a group of compounds frequently misused by athletes and, unfortunately, also by the general population, have lately attracted global attention; thus, significant demands for more precise, facile, and rapid AAS detection have arisen. The standard methods ordinarily used for AAS determination include liquid and gas chromatography coupled with mass spectrometry. However, good knowledge of steroid metabolism, pretreatment of samples (such as derivatization), and well-trained operators of the instruments are required, making this procedure expensive, complicated, and not routinely applicable. In the drive to meet current AAS detection demands, the scientific focus has shifted to developing novel, tailor-made approaches leading to time- and cost-effective, routine, and field-portable methods for AAS determination in various matrices, such as biological fluids, food supplements, meat, water, or other environmental components. Therefore, herein, we present a comprehensive review article covering recent advances in AAS determination, with a strong emphasis on the increasingly important role of chemically designed artificial sensors, biosensors, and antibody- and fluorescence-based methods.

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Section: Figurementioning

confidence: 99%

Advances in the Determination of Anabolic-Androgenic Steroids: From Standard Practices to Tailor-Designed Multidisciplinary Approaches

Huml

Tauchen

Rimpelová

et al. 2021

Sensors

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“…Supramolecular sensors, once limited to organic solvents and aqueous/organic mixtures, can now detect biologically relevant analytes even in salty aqueous solutions. − Analyte detection in water is made more difficult and less predictable when the target is found in complex biological media. Examples of supramolecular sensing in human cells, − enzyme-supporting buffers, − bacterial culture, and real biofluids − now exist, but the de novo design of new sensing systems that work in complex biological media remains nontrivial.…”

Section: Introductionmentioning

confidence: 99%

Parallel Synthesis and Screening of Supramolecular Chemosensors That Achieve Fluorescent Turn-on Detection of Drugs in Saliva

Beatty

Selinger

et al. 2019

J. Am. Chem. Soc.

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Programming and controlling molecular recognition in aqueous solutions is increasingly common, but creating supramolecular sensors that detect analytes in biologically relevant solutions remains a nontrivial task. We report here a parallel synthesis-driven approach to create a family of selfassembling dimeric sensors that we call DimerDyes and its use for the rapid identification of salt-tolerant sensors for illicit drugs. We developed an efficient method that involves parallel synthesis and screening in crude form without the need to purify each potential sensor. Structurally diverse "hit" DimerDyes were resynthesized and purified and were each shown to assemble into homodimers in water in the programmed way. DimerDyes provided a "turn-on" fluorescence detection of multiple illicit drugs at low micromolar concentrations in water and in saliva. The combination of multiple agents into a sensor array was successfully able to detect and discriminate between closely related drugs and metabolites in multiple important drug families.

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“…2 Detection methods that currently exist suffer from a variety of drawbacks, 3 including the need for expensive laboratory instrumentation and signicant sample preparation prior to analysis, that make them impractical for on-site usage at sporting events. 4 Although uorescence-based detection of steroids has been reported, such detection generally requires chromatography, 5 additional additives, 6 or expensive instrumentation, 7 which adds signicant time and cost to the analysis. As such, a new method is needed.…”

Section: Introductionmentioning

confidence: 99%

Detection of anabolic steroidsviacyclodextrin-promoted fluorescence modulation

Haynes

Levine

2020

RSC Adv.

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Selective Array‐Based Sensing of Anabolic Steroids in Aqueous Solution by Host–Guest Reporter Complexes

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.

Cited by 17 publications

References 51 publications

Advances in the Determination of Anabolic-Androgenic Steroids: From Standard Practices to Tailor-Designed Multidisciplinary Approaches

Advances in the Determination of Anabolic-Androgenic Steroids: From Standard Practices to Tailor-Designed Multidisciplinary Approaches

Parallel Synthesis and Screening of Supramolecular Chemosensors That Achieve Fluorescent Turn-on Detection of Drugs in Saliva

Detection of anabolic steroidsviacyclodextrin-promoted fluorescence modulation

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