Selective antibacterial activity of a novel lactotransferrin-derived antimicrobial peptide LF-1 against Streptococcus mutans

Feng, Zhongxin; Luo, Jingjie; Lyu, Xiaohui; Chen, Yue; Zhang, Linglin

doi:10.1016/j.archoralbio.2022.105446

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…S. mutans generally enters the mid-logarithmic phase within 6–8 h with the most vigorous growth speed [ 21 , 22 ], which is when S. mutans is most suitable for drug effect evaluation. Above all, we utilised MTT (a cell viability detection reagent) to investigate the effects of LF-1 at low concentrations on S. mutans growth at 6 h. Consistent with our previous results [ 20 ], LF-1 at 8 and 4 μmol/L almost inhibited S. mutans growth within 6 h. Unexpectedly, LF-1 at a low concentration (2 μmol/L) also significantly decreased S. mutans growth activity at this concentration but did not delay growth in the time-growth kinetic curve. It is convenient to investigate bacterial growth characteristics by measuring the optical density of bacterial suspensions, but the MTT method is more accurate in exploring differences that cannot be reflected in the time-growth kinetic curve.…”

Section: Discussionsupporting

confidence: 86%

“…Compared with oral commensal Streptococcus species ( S. sanguinis and S. gordonii ), LF-1 selectively targeted S. mutans with preferable membrane affinity. These results highlight the potential of LF-1 to fight S. mutans and dental caries [ 20 ]. However, the influence of LF-1 on cariogenic virulence factors of S. mutans remains elusive and requires further investigation.…”

Section: Introductionmentioning

confidence: 80%

“…LF-1 demonstrated the most notable antibacterial activity against the tested strains of S. mutans , with an MIC of 8 μmol/L. In addition, the higher membrane affinity of LF-1 for S. mutans than other oral commensal Streptococcus species contributes to better targeting of S. mutans [ 20 ]. Considering the selectivity of LF-1 against S. mutans and the crucial role of the cariogenic capacity of S. mutans , we further explored the effects of LF-1 on the cariogenic virulence factors of S. mutans [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

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Novel Lactotransferrin-Derived Antimicrobial Peptide LF-1 Inhibits the Cariogenic Virulence Factors of Streptococcus mutans

et al. 2023

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We previously developed a novel lactotransferrin-derived antimicrobial peptide, LF-1, with selective antibacterial activity against the characteristic cariogenic bacterium Streptococcus mutans. This study further investigated the effects of LF-1 on the cariogenic virulence factors of S. mutans and evaluated the changes in virulence-associated enzymes and genes; the viability, acidogenicity, and aciduricity of planktonic S. mutans; and initial colonisation and biofilm formation after treatment with LF-1. The method of qRT-PCR was used to evaluate S. mutans virulence-associated gene expression. LF-1 interfered with the cell viability of S. mutans within 6 h. LF-1 inhibited the acidogenicity and aciduricity of S. mutans, with reduced lactic acid production and survival in a lethal acidic environment, and inactivated lactate dehydrogenase and F1F0-ATPase activity. LF-1 decreased surface-adherent S. mutans within 60 min and inhibited S. mutans biofilm formation, where scanning electron microscopy and confocal laser scanning microscopy showed reduced extracellular matrix and bacteria. LF-1 downregulates S. mutans virulence-associated gene expression. LF-1 inhibited the growth and cariogenic virulence factors of S. mutans in vitro with a reduction in key enzymatic activity and downregulation of virulence-associated gene expression. LF-1 has promising application prospects in the fight against S. mutans and dental caries.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Novel Lactotransferrin-Derived Antimicrobial Peptide LF-1 Inhibits the Cariogenic Virulence Factors of Streptococcus mutans

et al. 2023

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“…21 Tonguc et al 22 suggested that there is a significant antibacterial effect of different saliva-substitutes-containing-lysozyme (LYZ) or LF on Streptococcus mutans (S. mutans) in comparison with human saliva. Feng et al 23 Selective antibacterial activity of a novel lactotransferrin-derived antimicrobial peptide LF-1 against S. mutans and concluded that LF-1 has selective affinity for and antibacterial activity against S. mutans with strong membrane disrupting ability, highlighting the potential of LF-1 as a crucial antibacterial agent in caries prevention. Pinheiro et al 24 concluded that the combination of hydroxyapatite with lysozyme, LF, and lactoperoxidase might be an alternative for S. mutans control in dentinal caries.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Levels of Salivary Lysozyme before and after Removable Prosthodontic Treatment among Partially Edentulous Patients: An Observational Study

Dhawan¹,

Kaur²,

Dogra³

et al. 2023

International Journal of Prosthodontics and Restorative Dentistry

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Purpose: Salivary biomarkers can present variation in the onset, progression, or even regression of a particular disorder. One such biomarker present in the oral cavity is salivary lysozyme which plays a significant role in the defense mechanism of our body. The purpose of this study was to determine the changes in the levels of salivary lysozyme before and after removable prosthetic treatment in partially edentulous patients. Materials and methods: A total of 40 subjects were enrolled for the study who had reported to the Department of Prosthodontics for a removable partial denture (RPD) of their partial edentulism. A simplified oral hygiene index (OHI-S) was recorded for each subject. The saliva samples were collected at different time intervals, that is, baseline before the removable prosthodontic treatment and 3 months after treatment. This was followed by a comparative analysis of changes in the levels of salivary lysozyme using enzyme-linked immunosorbent assay (ELISA). Categorical variables (like gender) were summarized as frequencies, while continuous variables were summarized as mean and standard deviation. Parametric tests of significance (paired t-test) were used for inferential statistics. Continuous data were tested by means of the Shapiro-Wilk test. Results:The mean value for the OHI-S before removable prosthodontic treatment was 2.41; however, after insertion of the RPD, the value was 3.04 suggesting a significant increase of 0.6225 (p < 0.001). The levels of salivary lysozyme increased essentially in patients after wearing an RPD for 3 months. The initial values were 11.27 ± 0.37, which elevated to 13.10 ± 0.39 in a span of 3 months. Conclusion:The inference of this study advocated that the salivary lysozyme levels may be of significance for the determination of susceptibility to the disease, its progression, or regression pre-and posttreatment following initial colonization of bacteria after the RPD insertion.

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“…Reduced biofilm growth resulted from an inhibitory effect on EPS synthesis and/or excretion [77]. S. mutans growth was also inhibited by the strong membrane disrupting activity of the lactotransferrin-derived AMP LF1 [78]. Boswell et al reported on the effect of lysozyme, lactoferrin, and LL-37 on S. pneumoniae serotype 23F, which is associated with respiratory infections.…”

Section: Enterococcus Faecalismentioning

confidence: 99%

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Fontanot,

Ellinger,

Unger

et al. 2024

Antibiotics

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Microbial biofilm formation creates a persistent and resistant environment in which microorganisms can survive, contributing to antibiotic resistance and chronic inflammatory diseases. Increasingly, biofilms are caused by multi-drug resistant microorganisms, which, coupled with a diminishing supply of effective antibiotics, is driving the search for new antibiotic therapies. In this respect, antimicrobial peptides (AMPs) are short, hydrophobic, and amphipathic peptides that show activity against multidrug-resistant bacteria and biofilm formation. They also possess broad-spectrum activity and diverse mechanisms of action. In this comprehensive review, 150 publications (from January 2020 to September 2023) were collected and categorized using the search terms ‘polypeptide antibiotic agent’, ‘antimicrobial peptide’, and ‘biofilm’. During this period, a wide range of natural and synthetic AMPs were studied, of which LL-37, polymyxin B, GH12, and Nisin were the most frequently cited. Furthermore, although many microbes were studied, Staphylococcus aureus and Pseudomonas aeruginosa were the most popular. Publications also considered AMP combinations and the potential role of AMP delivery systems in increasing the efficacy of AMPs, including nanoparticle delivery. Relatively few publications focused on AMP resistance. This comprehensive review informs and guides researchers about the latest developments in AMP research, presenting promising evidence of the role of AMPs as effective antimicrobial agents.

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Selective antibacterial activity of a novel lactotransferrin-derived antimicrobial peptide LF-1 against Streptococcus mutans

Cited by 9 publications

References 42 publications

Novel Lactotransferrin-Derived Antimicrobial Peptide LF-1 Inhibits the Cariogenic Virulence Factors of Streptococcus mutans

Novel Lactotransferrin-Derived Antimicrobial Peptide LF-1 Inhibits the Cariogenic Virulence Factors of Streptococcus mutans

Comparative Analysis of Levels of Salivary Lysozyme before and after Removable Prosthodontic Treatment among Partially Edentulous Patients: An Observational Study

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

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