Selective and single step adsorption of α-lactalbumin from whole cow's milk on hydroxyapatite microbeads

Çetinkaya, Sevil; Akkaya, Birnur

doi:10.1016/j.msec.2016.06.040

Cited by 6 publications

(2 citation statements)

References 42 publications

(39 reference statements)

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“…The selectivity is consecquence of needle-shaped HA morphology, with large surface area, in comparison to irregularly shaped HA. Cetınkaya et al [118] modified HA microbeads by embedding in ethylene glycol dimethacrylate (EGDMA) in the presence of pore makerwere, and used them to isolate ALA from cow's milk by a single step. ALA separation was performed without fat and casein removal steps.…”

Section: Hydroxyapatite (Ha)mentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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Background: The world production of whey was estimated to be more than 200 million tons per year. Although whey is an important source of proteins with high nutritional value and biotechnological importance, it is still considered as a by-product of the dairy industry with low economic value due to low industrial exploitation. There are several challenges in the separation of whey proteins: low concentration, the complexity of the material and similar properties (pI, molecular mass) of some proteins. Methods: A narrative review of all the relevant papers on the present methodologies based on ion-exchange and adsorption principles for isolation of whey proteins, known to the authors, was conducted. Results: Traditional ion-exchange techniques are widely used for the separation and purification of the bovine whey proteins. These methodologies, based on the anion or cation chromatographic procedures, as well as combination of aforementioned techniques are still preferential methods for the isolation of the whey proteins on the laboratory scale. However, more recent research on ion exchange membranes for this purpose has been introduced, with promising potential to be applied on the pilot industrial scale. Newly developed methodologies based either on the ion-exchange separation (for example: simulated moving bed chromatography, expanded bed adsorption, magnetic ion exchangers, etc.) or adsorption (for example: adsorption on hydroxyapatite or activated carbon, or molecular imprinting) are promising approaches for scaling up of the whey proteins’ purification processes. Conclusion: Many procedures based on ion exchange are successfully implemented for separation and purification of whey proteins, providing protein preparations of moderate-to-high yield and satisfactory purity. However, the authors anticipate further development of adsorption-based methodologies for separation of whey proteins by targeting the differences in proteins’ structures rather than targeting the differences in molecular masses and pI. The complex composite multilayered matrices, including also inorganic components, are promising materials for simultaneous exploiting of the differences in the masses, pI and structures of whey proteins for the separation.

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Section: Hydroxyapatite (Ha)mentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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“…However, in general, commercial WPI or WPC was used to optimise the methods. New methods were developed based on successive precipitations and separations by microfiltration and/or ultrafiltration (Alomirah & Alli, 2004;Arunkumar & Etzel, 2014;Toro-Sierra et al, 2013), selective enzymatic hydrolysis (Konrad & Kleinschmidt, 2008;Lisak, Toro-Sierra, Kulozik, Bozanic & Cheison, 2013), selective adsorption on hydroxyapatite microbeads (Cetinkaya & Akkaya, 2016), processes applying supercritical carbon dioxide Yver, Bonnaillie, Yee, Mcaloon & Tomasula, 2012), or methods based on two-phase systems (Sivakumar & Iyyaswami, 2015). These methods involve multiple steps to achieve high purity fractions (Table 1), which could hinder their implementation.…”

Section: Introductionmentioning

confidence: 99%

One-step chromatographic method to purify α-lactalbumin from whey for nanotube synthesis purposes

Fuciños

Estévez

et al. 2019

Food Chemistry

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A one-step anion-exchange chromatography method (NaCl gradient elution on a DEAE Sepharose™ Fast Flow gel column) was developed to purify α-lactalbumin (α-LA) from whey protein isolate. α-LA nearly 100% pure (based on the total protein content) was obtained with a yield of about 39%. Besides pure α-LA, which was the main objective of this work, highly pure β-lactoglobulin was also obtained with a yield of about 59%. The high purity of the obtained α-LA samples allowed its use to synthesise protein nanotubes with excellent gelation properties for their use as food thickeners and bioactive carriers. The samples' purity degree obtained (based on the total protein content) was critical in the formation of proper nanotubes instead of random aggregates, which produced opaque and weak gels, less useful for food applications.

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A new effective nano-adsorbent and antibacterial material of hydroxyapatite

et al. 2018

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Selective and single step adsorption of α-lactalbumin from whole cow's milk on hydroxyapatite microbeads

Cited by 6 publications

References 42 publications

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

One-step chromatographic method to purify α-lactalbumin from whey for nanotube synthesis purposes

A new effective nano-adsorbent and antibacterial material of hydroxyapatite

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