Selective and sensitive fluorometric determinations of cobalt(II) and hydrogen peroxide with fluorescein-hydrazide

Mori, Itsuo; Takasaki, K.; Fujita, Yoshikazu; Matsuo, Takako

doi:10.1016/s0039-9140(98)00118-0

Cited by 66 publications

(23 citation statements)

References 12 publications

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“…Therefore, rapid, accurate and reliable detection of H 2 O 2 is of great significance to not only scientific research but also practical applications. Compared with many traditional detection approaches [5][6][7][8], the newly emerging electrochemical methods have attracted much attention because of its high sensitivity, fast detection, low cost, and ease of operation [9]. So far, electrochemical sensors based on enzymes have been fully developed due to their high sensitivity and extremely good selectivity.…”

Section: Introductionmentioning

confidence: 99%

Non-enzymatic amperometric detection of hydrogen peroxide using grass-like copper oxide nanostructures calcined in nitrogen atmosphere

Gao¹,

Gong²,

Mellott³

et al. 2015

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

Non-enzymatic amperometric detection of hydrogen peroxide using grass-like copper oxide nanostructures calcined in nitrogen atmosphere

Gao¹,

Gong²,

Mellott³

et al. 2015

Electrochimica Acta

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“…Hence, we developed a handy and highly sensitivity method for the determination of ROS, including H 2 O 2 . We have already developed methods for the fluorometric determination of cobalt(II) and H 2 O 2 with fluorescein hydrazide (FH), 21) however, the method adopted for the determination of H 2 O 2 exhibited remarkably poor reproducibility. In this study, method to obtain a more reproducible and sensitive spectrofluorimetric determination of H 2 O 2 and other ROS were examined.…”

mentioning

confidence: 99%

Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

et al. 2008

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977Reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ), superoxide (O 2 Ϫ ), hydroxyl radical ( · OH), nitric oxide (NO), peroxynitrite (ONOO Ϫ ), and peroxide (R-OOH) affect the living body and cause various types of diseases such as cancer, 1) cardiovascular disorders, 2) and neurodegenerative diseases. 3,4) On the other hand, it is accepted that some ROS control disinfection in cells and work as a biofactor for signal transmission of insulin. 5) In addition, H 2 O 2 is a major by-product of ROS in living organisms and a common marker for oxidative stress in the field of clinical inspection.6-8) The development of methods for the determination of these substances is significant in the fields of clinical and biological studies. Several methods for the determination of the spectrophotometry, 9-11) fluorophotometry, [12][13][14][15][16][17][18] and chemiluminescence 19,20) of H 2 O 2 and other ROS have already been reported. These methods focus on the determination of individual ROS. However, it appears that most of ROS have only minute life times under biological environments. Therefore, we considered that it is more practicable to measure the total rather than individual ROS for a clinical purpose. Hence, we developed a handy and highly sensitivity method for the determination of ROS, including H 2 O 2 . We have already developed methods for the fluorometric determination of cobalt(II) and H 2 O 2 with fluorescein hydrazide (FH), 21) however, the method adopted for the determination of H 2 O 2 exhibited remarkably poor reproducibility. In this study, method to obtain a more reproducible and sensitive spectrofluorimetric determination of H 2 O 2 and other ROS were examined. Furthermore, the applicability of the method for measuring H 2 O 2 in urine samples was examined. Moreover, because an excellent crystal of FH was obtained, structural analysis by X-ray diffractometry was performed. As a result, this FH crystal was confirmed to have a five-membered spirolactam structure. The relationship between fluorescence and the chemical structure was studied; the fluorescein, which was the strong fluorescent substance that caused the spirolactam formation by acting as the hydrazine conductor, became a colorless, non-fluorescent substance. Experimental Synthesis of Fluorescein Hydrazide (FH)A modification of the procedure of Baeyer and Akita. 22,23) for the synthesis of fluoescein and FH was employed. Resorcinol 2 mol and phthalic anhydride 1 mol were combined with methanesulfonic acid. The mixture was heated and dissolved in 5% sodium hydroxide solution. The solution was poured into 30% acetic acid; the resulting fluorescein, a yellow precipitate, was collected with a yield of 92.5%. Subsequently, fluorescein was added to a solution of excess 98% anhydrous hydrazine and heated at 80°C in a warm water bath for 3 h. Upon cooling to room temperature and with the addition of strong hydrochloric acid into the solution, the light yellow substance was deposited. Then, the substance obtained was dissolved in ethan...

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“…1,2 Many analytical methods have been reported for such measurements, including electroanalytical techniques, [3][4][5] spectrophotometry, 6,7 chemiluminescence, 8,9 and fluorometry. [10][11][12] Among these methods, most are based on the enzyme (peroxidase and catalase) catalytic reaction. Enzymatic determinations of H2O2 have drawn increasing interest due to their unusual sensitivity, selectivity and simplicity.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Determination of Hydrogen Peroxide Using Hemoglobin as a Mimetic Enzyme of Peroxidase

Zhang

2001

ANAL. SCI.

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The determination of hydrogen peroxide is of great importance, ascribable to both the fact that it is a product of reactions catalyzed by a large number of oxidase enzymes, and that it is essential in food, pharmaceutical, environmental, and clinical analyses. 1,2 Many analytical methods have been reported for such measurements, including electroanalytical techniques, 3-5 spectrophotometry, 6,7 chemiluminescence, 8,9 and fluorometry.10-12 Among these methods, most are based on the enzyme (peroxidase and catalase) catalytic reaction. Enzymatic determinations of H2O2 have drawn increasing interest due to their unusual sensitivity, selectivity and simplicity. Horse radish peroxidase (HRP), in which the heme iron acts as the activity center, is the most commonly used enzyme in H2O2 detection. However, the instability and high cost of the natural enzyme has stimulated people to search for alternatives. So far, peroxidase mimics that had been applied to the determination of H2O2 have been based on two kinds of substances: one is small bio-molecules from bovine blood, such as hemin and hematin, 10,13,14 and the other is the synthesized metal complexes, such as metal-porphyrin complexes, [15][16][17] metalphthalocyanines. 18,19 However, simple, natural or synthetic metal-complexes do not show satisfactory activity and selectivity because they lack the spatial structure of the natural enzyme, which is essential for the special inclusion behavior between the enzyme and the substrate.Hemoglobin (Hb), a necessary vehicle for oxygen carriage, has the natural quaternary structure. It contains four subunits of polypeptide, and each polypeptide chain contains a heme group that serves as the active center. Thus Hb can be a mimetic enzyme of HRP, 7,20 and the catalytic activity of Hb is much higher than hemin, β-CD-hemin and some metalloporphyrins because Hb has the natural quaternary structure. 7,20 Furthermore, Hb is very stable, and it is worth to mentioning that the price of Hb is 500-times less than that of HRP. There is little doubt that Hb can be effectively substituted for HRP for H2O2 determination. However, to the best of our knowledge, the application of Hb as mimetic peroxidase to a fluorescence analysis of H2O2 has not yet been reported. In this paper, a novel fluorometry for hydrogen peroxide determination is presented, in which hemoglobin (Hb) was used as mimetic enzyme of peroxidase for fluorogenic reaction between an inexpensive substrate thiamine and H2O2. Under the catalysis of Hb, thiamin was oxidized by H2O2 to a fluorescent product, thiochrome, which exhibited strong fluorescence at 440 nm with an excitation wavelength of 370 nm. The obtained result demonstrates that Hb is a promising peroxidase mimic. The applicability of the catalytic reaction was adopted to the determination of H2O2 in rainwater. Satisfactory results were obtained. Experimental ReagentsHb (bovine) solutions were prepared by dissolving a certain amount of Hb (Sino-American Biotechnology Co., China) in water and stored in refrigerator (4...

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Selective and sensitive fluorometric determinations of cobalt(II) and hydrogen peroxide with fluorescein-hydrazide

Cited by 66 publications

References 12 publications

Non-enzymatic amperometric detection of hydrogen peroxide using grass-like copper oxide nanostructures calcined in nitrogen atmosphere

Non-enzymatic amperometric detection of hydrogen peroxide using grass-like copper oxide nanostructures calcined in nitrogen atmosphere

Fluorophotometric Determination of Hydrogen Peroxide and Other Reactive Oxygen Species with Fluorescein Hydrazide (FH) and Its Crystal Structure

Fluorescence Determination of Hydrogen Peroxide Using Hemoglobin as a Mimetic Enzyme of Peroxidase

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