Selective and sensitive electrochemical measurement of nitric oxide in aqueous solution: discussion and new results

Lantoine, Frédérique; Trevin, Stéphane; Bedioui, Féthi; Devynck, Jacques

doi:10.1016/0022-0728(95)04042-m

Cited by 152 publications

(116 citation statements)

References 22 publications

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“…Nitric oxide measurement NO standards were prepared by serial dilutions of saturated NO solutions, as previously described (Lantoine et al, 1995b). Saturated NO solutions were prepared under oxygen-free conditions by successive bubbling with argon for 20 min, then with pure NO gas (Aldrich) for 20 min.…”

Section: Platelet Preparationmentioning

confidence: 99%

Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets

Sang

Lantoine

Devynck

1996

British J Pharmacology

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Nitric oxide (NO) donors inhibit platelet function and Ca2+ mobilization evoked by different agonists. This led us to investigate the direct effects of authentic NO on basal cytosolic Ca2+ concentration ([Ca2+]i) and on Ca2+ mobilization induced by thrombin or by two inhibitors of intracellular Ca2+‐ATPases, thapsigargin and 2,5‐di‐(t‐butyl)‐l,4‐benzohydroquinone (t‐BuBHQ). Cytosolic Ca2+ concentration was evaluated with Fura‐2, in the absence of Ca2+ influx. Addition of 5 μm NO increased by 48% the basal cytosolic [Ca2+] of resting human platelets whereas a lower concentration (0.1 μm) did not induce significant modifications. This NO‐induced Ca2+ increase was inversely correlated with the basal level of cytosolic [Ca2+]. NO pretreatment for 30 to 120 s decreased by 42 to 57% the transient [Ca2+]i peak evoked by 0.10 u ml−1 thrombin and strongly attenuated the initial rate of [Ca2+]i rise induced by 1 μm thapsigargin or by 20 μm t‐BuBHQ. The two components of the thapsigargin response, the Ca2+ release due to inhibition of Ca2+ pumps and the thromboxane A2‐dependent self‐amplification mechanism, were inhibited by NO. The observation that a subsequent stimulation was still capable of eliciting Ca2+ release suggests the presence of NO‐insensitive Ca2+ stores. These findings indicate that nitric oxide can modulate basal cytosolic [Ca2+] in unstimulated human platelets and decrease the Ca2+ mobilization from NO‐sensitive internal stores evoked by stimulation of receptors or by Ca2+‐ATPase inhibitors. This underlines the important role of nitric oxide in the modulation of platelet Ca2+ handling.

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Section: Platelet Preparationmentioning

confidence: 99%

Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets

Sang

Lantoine

Devynck

1996

British J Pharmacology

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“…The first is based on the acidic Griess reagent assay [46][47][48][49] and the second on the conversion of oxyhaemoglobin to methaemoglobin by NO [50][51][52]. However, both methods have inherent difficulties.…”

Section: Analysis Of No Stock Solutionmentioning

confidence: 99%

“…Cserey and Gratzl [45] have reported a stock NO concentration of 2.4±0.3 mmol L À1 at room temperature which was generated by bubbling buffer with chemically generated NO obtained by reacting KNO 2 with excess ascorbic acid at a pH lower than 3. Lantoine et al [48] have previously worked with NO stock solutions with a concentration of 1.4 mmol L À1 at room temperature. Their stock NO solution was produced by bubbling PBS with pure NO gas (Aldrich Chemical Co., St Louis, MO).…”

Section: Electrochemical Sensor Studiesmentioning

confidence: 99%

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV?visible spectroscopy for determining stock concentrations

et al. 2005

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The increasing scientific interest in nitric oxide (NO) necessitates the development of novel and simple methods of synthesising NO on a laboratory scale. In this study we have refined and developed a method of NO synthesis, using the neutral Griess reagent, which is inexpensive, simple to perform, and provides a reliable method of generating NO gas for in-vivo sensor calibration. The concentration of the generated NO stock solution was determined using UV-visible spectroscopy to be 0.28±0.01 mmol L À1 . The level of NO 2 À contaminant, also determined using spectroscopy, was found to be 0.67±0.21 mmol L À1 . However, this is not sufficient to cause any considerable increase in oxidation current when the NO stock solution is used for electrochemical sensor calibration over physiologically relevant concentrations; the NO sensitivity of bare Pt-disk electrodes operating at +900 mV (vs. SCE) was 1.08 nA lmol À1 L, while that for NO 2 À was 5.9·10 À3 nA lmol À1 L. The stability of the NO stock solution was also monitored for up to 2 h after synthesis and 30 min was found to be the time limit within which calibrations should be performed.

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“…Then the solution was bubbled with pure NO gas for 30 minutes. The concentration of a saturated NO solution is 1.4 mM [21][22]. Standards were freshly made for each experiment and kept in a glass flask with a rubber septum.…”

Section: Chemicalsmentioning

confidence: 99%

“…Dilutions of the saturated solution were made using deoxygenated HPLC grade water. [21] Standard stock SNAP solution [19,27]: 500 ml of HPLC grade water was adjusted to pH 9.0; before dissolving 7 mg of EDTA; deoxygenated with pure nitrogen for 20 min. 11.0 mg SNAP was finally added to the mixture and the resultant solution refrigerated in the dark (light-sensitive, slowly decomposes at room temperature).…”

Section: Chemicalsmentioning

confidence: 99%

The Electrochemical Detremination of Nitric Oxide in Seawater Media with Microelectrodes

Zhengbin

Xing

Jiang

et al. 2003

Sensors

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Abstract:The electrochemical determination of nitric oxide in seawater with microelectrodes is reported in this paper. Two electrodes, ISO-NOPMC microsensor and modified platinum microelectrode, are evaluated for their performance in seawater. The peak current is found to be linear with the NO concentration in the range 1×10 -6 -1.2×10-5 mol/l for both microelectrodes. The detection limit of ISO-NOPMC microsensor is 1.4×10 -8 mol/l. Two methods for calibration of NO sensor, saturated NO solutions and decomposition of S-nitroso-acetyl-DL-penicillamine (SNAP), are discussed and compared. In addition, we examined the performance degradation of these microelectrodes in seawater due to complexation with seawater components.

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Selective and sensitive electrochemical measurement of nitric oxide in aqueous solution: discussion and new results

Cited by 152 publications

References 22 publications

Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets

Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV?visible spectroscopy for determining stock concentrations

The Electrochemical Detremination of Nitric Oxide in Seawater Media with Microelectrodes

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Selective and sensitive electrochemical measurement of nitric oxide in aqueous solution: discussion and new results

Cited by 152 publications

References 22 publications

Influence of authentic nitric oxide on basal cytosolic [Ca2+] and Ca2+ release from internal stores in human platelets

Influence of authentic nitric oxide on basal cytosolic [Ca2+] and Ca2+ release from internal stores in human platelets

Calibration of NO sensors for in-vivo voltammetry: laboratory synthesis of NO and the use of UV?visible spectroscopy for determining stock concentrations

The Electrochemical Detremination of Nitric Oxide in Seawater Media with Microelectrodes

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Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets

Influence of authentic nitric oxide on basal cytosolic [Ca²⁺] and Ca²⁺ release from internal stores in human platelets